Restriction map for
pF1KE2455
(Linear) (Six-base) MAPSORT of: KIEE2455.seq Check: 5771 from: 1 to: 1437
KIEE2455.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 312 1437
Size: 312 1125
Acc65I G'GTAC_C
Cuts at: 0 266 1203 1437
Size: 266 937 234
Fragments arranged by size:
937 266 234
AleI CACnn'nnGTG
Cuts at: 0 1270 1437
Size: 1270 167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 1437
Size: 166 1271
ApaI G_GGCC'C
Cuts at: 0 46 1127 1437
Size: 46 1081 310
Fragments arranged by size:
1081 310 46
ApoI r'AATT_y
Cuts at: 0 481 1437
Size: 481 956
AvaI C'yCGr_G
Cuts at: 0 45 375 782 871 1437
Size: 45 330 407 89 566
Fragments arranged by size:
566 407 330 89 45
BanI G'GyrC_C
Cuts at: 0 266 295 1203 1437
Size: 266 29 908 234
Fragments arranged by size:
908 266 234 29
BanII G_rGCy'C
Cuts at: 0 46 1127 1437
Size: 46 1081 310
Fragments arranged by size:
1081 310 46
BbvCI CC'TCA_GC
Cuts at: 0 588 1437
Size: 588 849
BglI GCCn_nnn'nGGC
Cuts at: 0 763 1437
Size: 763 674
Bme1580I G_kGCm'C
Cuts at: 0 26 46 1127 1437
Size: 26 20 1081 310
Fragments arranged by size:
1081 310 26 20
BmgBI CAC'GTC
Cuts at: 0 339 1437
Size: 339 1098
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 297 603 1437
Size: 297 306 834
Fragments arranged by size:
834 306 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 721 1437
Size: 712 9 716
Fragments arranged by size:
716 712 9
Bpu10I CC'TnA_GC
Cuts at: 0 574 588 1437
Size: 574 14 849
Fragments arranged by size:
849 574 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1437
Size: 397 1040
BsaI GGTCTCn'nnnn_
Cuts at: 0 919 1437
Size: 919 518
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1219 1249 1437
Size: 1219 30 188
Fragments arranged by size:
1219 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 517 1437
Size: 514 3 920
Fragments arranged by size:
920 514 3
BseYI C'CCAG_C
Cuts at: 0 389 832 970 1069 1437
Size: 389 443 138 99 368
Fragments arranged by size:
443 389 368 138 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1437
Size: 282 1155
BsiEI CG_ry'CG
Cuts at: 0 40 1437
Size: 40 1397
BsmBI CGTCTCn'nnnn_
Cuts at: 0 252 1437
Size: 252 1185
Bsp1286I G_dGCh'C
Cuts at: 0 26 46 1127 1437
Size: 26 20 1081 310
Fragments arranged by size:
1081 310 26 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 312 1437
Size: 254 58 1125
Fragments arranged by size:
1125 254 58
BsrDI GCAATG_nn'
Cuts at: 0 1161 1437
Size: 1161 276
BsrFI r'CCGG_y
Cuts at: 0 13 1437
Size: 13 1424
BsrGI T'GTAC_A
Cuts at: 0 1014 1437
Size: 1014 423
BstXI CCAn_nnnn'nTGG
Cuts at: 0 277 1437
Size: 277 1160
BstYI r'GATC_y
Cuts at: 0 254 966 1437
Size: 254 712 471
Fragments arranged by size:
712 471 254
Bsu36I CC'TnA_GG
Cuts at: 0 1169 1437
Size: 1169 268
BtgI C'CryG_G
Cuts at: 0 192 237 1194 1268 1437
Size: 192 45 957 74 169
Fragments arranged by size:
957 192 169 74 45
BtsI GCAGTG_nn'
Cuts at: 0 887 1437
Size: 887 550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1179 1214 1437
Size: 1179 35 223
Fragments arranged by size:
1179 223 35
EaeI y'GGCC_r
Cuts at: 0 11 37 99 1277 1437
Size: 11 26 62 1178 160
Fragments arranged by size:
1178 160 62 26 11
EagI C'GGCC_G
Cuts at: 0 37 1437
Size: 37 1400
EarI CTCTTCn'nnn_
Cuts at: 0 491 1303 1437
Size: 491 812 134
Fragments arranged by size:
812 491 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 1437
Size: 154 1283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 721 1437
Size: 712 9 716
Fragments arranged by size:
716 712 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 1388 1437
Size: 100 1288 49
Fragments arranged by size:
1288 100 49
EcoO109I rG'GnC_Cy
Cuts at: 0 1123 1124 1437
Size: 1123 1 313
Fragments arranged by size:
1123 313 1
HaeII r_GCGC'y
Cuts at: 0 20 1437
Size: 20 1417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 607 627 639 776 808 1036 1068
Size: 595 12 20 12 137 32 228 32
Cuts at: 1068 1437
Size: 369
Fragments arranged by size:
595 369 228 137 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 114 1437
Size: 114 1323
KpnI G_GTAC'C
Cuts at: 0 270 1207 1437
Size: 270 937 230
Fragments arranged by size:
937 270 230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1061 1154 1437
Size: 902 159 93 283
Fragments arranged by size:
902 283 159 93
MslI CAynn'nnrTG
Cuts at: 0 892 1270 1437
Size: 892 378 167
Fragments arranged by size:
892 378 167
MspA1I CmG'CkG
Cuts at: 0 78 150 225 389 690 918 1283 1437
Size: 78 72 75 164 301 228 365 154
Fragments arranged by size:
365 301 228 164 154 78 75 72
NaeI GCC'GGC
Cuts at: 0 15 1437
Size: 15 1422
NdeI CA'TA_TG
Cuts at: 0 1304 1437
Size: 1304 133
NgoMIV G'CCGG_C
Cuts at: 0 13 1437
Size: 13 1424
NspI r_CATG'y
Cuts at: 0 384 1437
Size: 384 1053
PspOMI G'GGCC_C
Cuts at: 0 42 1123 1437
Size: 42 1081 314
Fragments arranged by size:
1081 314 42
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 620 652 1437
Size: 620 32 785
Fragments arranged by size:
785 620 32
PstI C_TGCA'G
Cuts at: 0 1288 1437
Size: 1288 149
PvuII CAG'CTG
Cuts at: 0 225 389 690 918 1283 1437
Size: 225 164 301 228 365 154
Fragments arranged by size:
365 301 228 225 164 154
SapI GCTCTTCn'nnn_
Cuts at: 0 1303 1437
Size: 1303 134
SfcI C'TryA_G
Cuts at: 0 1284 1356 1371 1437
Size: 1284 72 15 66
Fragments arranged by size:
1284 72 66 15
SmaI CCC'GGG
Cuts at: 0 47 377 873 1437
Size: 47 330 496 564
Fragments arranged by size:
564 496 330 47
SmlI C'TyrA_G
Cuts at: 0 412 1437
Size: 412 1025
StyI C'CwwG_G
Cuts at: 0 1426 1437
Size: 1426 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1365 1437
Size: 1365 72
TatI w'GTAC_w
Cuts at: 0 786 1014 1437
Size: 786 228 423
Fragments arranged by size:
786 423 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 933 965 1437
Size: 933 32 472
Fragments arranged by size:
933 472 32
Tth111I GACn'n_nGTC
Cuts at: 0 421 1384 1437
Size: 421 963 53
Fragments arranged by size:
963 421 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 1437
Size: 846 591
XmaI C'CCGG_G
Cuts at: 0 45 375 871 1437
Size: 45 330 496 566
Fragments arranged by size:
566 496 330 45
Enzymes that do cut:
AarI Acc65I AleI AlfI ApaI ApoI AvaI BanI
BanII BbvCI BglI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I
BtgI BtsI CspCI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI MslI
MspA1I NaeI NdeI NgoMIV NspI PspOMI PsrI PstI
PvuII SapI SfcI SmaI SmlI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BsaAI BsaBI BsaHI BsaWI
BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII
DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MscI NarI NcoI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI