Restriction map for
pF1KE2445
(Linear) (Six-base) MAPSORT of: KIEE2445.seq Check: 9558 from: 1 to: 1347
KIEE2445.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 1201 1347
Size: 669 532 146
Fragments arranged by size:
669 532 146
AflII C'TTAA_G
Cuts at: 0 97 1347
Size: 97 1250
AflIII A'CryG_T
Cuts at: 0 1165 1347
Size: 1165 182
AhdI GACnn_n'nnGTC
Cuts at: 0 137 1347
Size: 137 1210
AleI CACnn'nnGTG
Cuts at: 0 60 1347
Size: 60 1287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 78 1347
Size: 78 1269
AlwNI CAG_nnn'CTG
Cuts at: 0 201 1347
Size: 201 1146
ApoI r'AATT_y
Cuts at: 0 103 645 764 867 970 1347
Size: 103 542 119 103 103 377
Fragments arranged by size:
542 377 119 103 103 103
BanI G'GyrC_C
Cuts at: 0 1079 1347
Size: 1079 268
BglII A'GATC_T
Cuts at: 0 31 567 1239 1347
Size: 31 536 672 108
Fragments arranged by size:
672 536 108 31
Bme1580I G_kGCm'C
Cuts at: 0 1275 1347
Size: 1275 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 804 1137 1347
Size: 804 333 210
Fragments arranged by size:
804 333 210
Bpu10I CC'TnA_GC
Cuts at: 0 1177 1347
Size: 1177 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1113 1347
Size: 548 565 234
Fragments arranged by size:
565 548 234
BsaI GGTCTCn'nnnn_
Cuts at: 0 429 1347
Size: 429 918
BsaBI GATnn'nnATC
Cuts at: 0 542 1297 1347
Size: 542 755 50
Fragments arranged by size:
755 542 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 686 716 1347
Size: 686 30 631
Fragments arranged by size:
686 631 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4 1347
Size: 4 1343
BseYI C'CCAG_C
Cuts at: 0 1012 1329 1347
Size: 1012 317 18
Fragments arranged by size:
1012 317 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 1347
Size: 501 846
Bsp1286I G_dGCh'C
Cuts at: 0 1275 1347
Size: 1275 72
BspHI T'CATG_A
Cuts at: 0 1035 1202 1347
Size: 1035 167 145
Fragments arranged by size:
1035 167 145
BstYI r'GATC_y
Cuts at: 0 31 197 567 1239 1347
Size: 31 166 370 672 108
Fragments arranged by size:
672 370 166 108 31
Bsu36I CC'TnA_GG
Cuts at: 0 998 1347
Size: 998 349
BtgI C'CryG_G
Cuts at: 0 23 579 1347
Size: 23 556 768
Fragments arranged by size:
768 556 23
BtsI GCAGTG_nn'
Cuts at: 0 513 1221 1347
Size: 513 708 126
Fragments arranged by size:
708 513 126
DraI TTT'AAA
Cuts at: 0 1007 1347
Size: 1007 340
EaeI y'GGCC_r
Cuts at: 0 26 1185 1347
Size: 26 1159 162
Fragments arranged by size:
1159 162 26
EarI CTCTTCn'nnn_
Cuts at: 0 427 1185 1261 1347
Size: 427 758 76 86
Fragments arranged by size:
758 427 86 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 1201 1347
Size: 669 532 146
Fragments arranged by size:
669 532 146
EcoO109I rG'GnC_Cy
Cuts at: 0 504 1347
Size: 504 843
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 559 591 820 852 1184 1216
Size: 123 32 404 32 229 32 332 32
Cuts at: 1216 1347
Size: 131
Fragments arranged by size:
404 332 229 131 123 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 144 600 1347
Size: 144 456 747
Fragments arranged by size:
747 456 144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1347
Size: 310 1037
MscI TGG'CCA
Cuts at: 0 28 1347
Size: 28 1319
MslI CAynn'nnrTG
Cuts at: 0 60 584 1259 1347
Size: 60 524 675 88
Fragments arranged by size:
675 524 88 60
NspI r_CATG'y
Cuts at: 0 122 237 739 1347
Size: 122 115 502 608
Fragments arranged by size:
608 502 122 115
PfoI T'CCnGG_A
Cuts at: 0 200 451 469 666 1347
Size: 200 251 18 197 681
Fragments arranged by size:
681 251 200 197 18
PpuMI rG'GwC_Cy
Cuts at: 0 504 1347
Size: 504 843
PsiI TTA'TAA
Cuts at: 0 358 1347
Size: 358 989
SmlI C'TyrA_G
Cuts at: 0 97 563 1092 1347
Size: 97 466 529 255
Fragments arranged by size:
529 466 255 97
SphI G_CATG'C
Cuts at: 0 237 1347
Size: 237 1110
SspI AAT'ATT
Cuts at: 0 365 624 677 1347
Size: 365 259 53 670
Fragments arranged by size:
670 365 259 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 625 1347
Size: 64 561 722
Fragments arranged by size:
722 561 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 686 718 1347
Size: 686 32 629
Fragments arranged by size:
686 629 32
XbaI T'CTAG_A
Cuts at: 0 750 897 1347
Size: 750 147 450
Fragments arranged by size:
750 450 147
XmnI GAAnn'nnTTC
Cuts at: 0 983 1214 1347
Size: 983 231 133
Fragments arranged by size:
983 231 133
Enzymes that do cut:
AcuI AflII AflIII AhdI AleI AlfI AlwNI ApoI
BanI BglII Bme1580I BmrI Bpu10I BpuEI BsaI BsaBI
BsaXI BseRI BseYI BsgI Bsp1286I BspHI BstYI Bsu36I
BtgI BtsI DraI EaeI EarI Eco57MI EcoO109I Hin4I
HindIII MmeI MscI MslI NspI PfoI PpuMI PsiI
SmlI SphI SspI TaqII TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BplI BpmI BsaAI
BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI StyI SwaI TatI
Tth111I XcmI XhoI XmaI ZraI