Restriction map for
pF1KE2444
(Linear) (Six-base) MAPSORT of: KIEE2444.seq Check: 6110 from: 1 to: 1323
KIEE2444.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 491 1323
Size: 491 832
AflIII A'CryG_T
Cuts at: 0 356 1323
Size: 356 967
AlwNI CAG_nnn'CTG
Cuts at: 0 1164 1257 1323
Size: 1164 93 66
Fragments arranged by size:
1164 93 66
ApoI r'AATT_y
Cuts at: 0 124 241 368 438 633 1217 1323
Size: 124 117 127 70 195 584 106
Fragments arranged by size:
584 195 127 124 117 106 70
BanII G_rGCy'C
Cuts at: 0 1271 1323
Size: 1271 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 650 1323
Size: 515 135 673
Fragments arranged by size:
673 515 135
BglII A'GATC_T
Cuts at: 0 80 1323
Size: 80 1243
BlpI GC'TnA_GC
Cuts at: 0 697 1323
Size: 697 626
BmgBI CAC'GTC
Cuts at: 0 36 1323
Size: 36 1287
BmrI ACTGGGnnnn_n'
Cuts at: 0 963 1323
Size: 963 360
BsaBI GATnn'nnATC
Cuts at: 0 521 1323
Size: 521 802
BsaHI Gr'CG_yC
Cuts at: 0 16 1323
Size: 16 1307
BseYI C'CCAG_C
Cuts at: 0 1270 1323
Size: 1270 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1323
Size: 1166 157
BsmBI CGTCTCn'nnnn_
Cuts at: 0 23 1323
Size: 23 1300
Bsp1286I G_dGCh'C
Cuts at: 0 1271 1323
Size: 1271 52
BspHI T'CATG_A
Cuts at: 0 372 1017 1323
Size: 372 645 306
Fragments arranged by size:
645 372 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 491 1323
Size: 491 832
BsrDI GCAATG_nn'
Cuts at: 0 103 464 691 1037 1323
Size: 103 361 227 346 286
Fragments arranged by size:
361 346 286 227 103
BstYI r'GATC_y
Cuts at: 0 80 1323
Size: 80 1243
Bsu36I CC'TnA_GG
Cuts at: 0 1037 1323
Size: 1037 286
BtgI C'CryG_G
Cuts at: 0 334 856 1323
Size: 334 522 467
Fragments arranged by size:
522 467 334
ClaI AT'CG_AT
Cuts at: 0 401 1323
Size: 401 922
DraI TTT'AAA
Cuts at: 0 296 420 546 1323
Size: 296 124 126 777
Fragments arranged by size:
777 296 126 124
EarI CTCTTCn'nnn_
Cuts at: 0 953 1323
Size: 953 370
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1241 1323
Size: 1241 82
EcoRI G'AATT_C
Cuts at: 0 368 1323
Size: 368 955
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 298 330 999 1031 1323
Size: 25 32 241 32 669 32 292
Fragments arranged by size:
669 292 241 32 32 32 25
HincII GTy'rAC
Cuts at: 0 1118 1323
Size: 1118 205
HindIII A'AGCT_T
Cuts at: 0 725 1323
Size: 725 598
MfeI C'AATT_G
Cuts at: 0 490 702 1323
Size: 490 212 621
Fragments arranged by size:
621 490 212
MslI CAynn'nnrTG
Cuts at: 0 119 839 1323
Size: 119 720 484
Fragments arranged by size:
720 484 119
MspA1I CmG'CkG
Cuts at: 0 528 1274 1323
Size: 528 746 49
Fragments arranged by size:
746 528 49
NcoI C'CATG_G
Cuts at: 0 856 1323
Size: 856 467
NspI r_CATG'y
Cuts at: 0 360 1323
Size: 360 963
PciI A'CATG_T
Cuts at: 0 356 1323
Size: 356 967
PflMI CCAn_nnn'nTGG
Cuts at: 0 1277 1323
Size: 1277 46
PstI C_TGCA'G
Cuts at: 0 557 1235 1323
Size: 557 678 88
Fragments arranged by size:
678 557 88
PvuII CAG'CTG
Cuts at: 0 528 1274 1323
Size: 528 746 49
Fragments arranged by size:
746 528 49
ScaI AGT'ACT
Cuts at: 0 1024 1323
Size: 1024 299
SfcI C'TryA_G
Cuts at: 0 553 1066 1231 1323
Size: 553 513 165 92
Fragments arranged by size:
553 513 165 92
SspI AAT'ATT
Cuts at: 0 791 1301 1323
Size: 791 510 22
Fragments arranged by size:
791 510 22
StyI C'CwwG_G
Cuts at: 0 230 856 1323
Size: 230 626 467
Fragments arranged by size:
626 467 230
TatI w'GTAC_w
Cuts at: 0 475 627 1022 1323
Size: 475 152 395 301
Fragments arranged by size:
475 395 301 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 20 52 1323
Size: 20 32 1271
Fragments arranged by size:
1271 32 20
XmnI GAAnn'nnTTC
Cuts at: 0 1171 1323
Size: 1171 152
Enzymes that do cut:
AarI AflIII AlwNI ApoI BanII BbsI BglII BlpI
BmgBI BmrI BsaBI BsaHI BseYI BsgI BsmBI Bsp1286I
BspHI BspMI BsrDI BstYI Bsu36I BtgI ClaI DraI
EarI EcoNI EcoRI Hin4I HincII HindIII MfeI MslI
MspA1I NcoI NspI PciI PflMI PstI PvuII ScaI
SfcI SspI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI Bme1580I BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BsiEI BsiHKAI BsiWI BsmI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI