(Linear) (Six-base) MAPSORT of: KIEE2434.seq  Check: 1526  from: 1  to: 873



KIEE2434.orf

 With 182 enzymes: SgfI * 

                        December 4, 2012 11:59  ..

AccI GT'mk_AC

Cuts at:      0     174     873 
   Size:        174     699
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     873 
   Size:        413     460
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     686     873 
   Size:        686     187
 
ApoI r'AATT_y

Cuts at:      0     256     362     452     513     873 
   Size:        256     106      90      61     360

  Fragments arranged by size: 

                 360     256     106      90      61
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      15     873 
   Size:         15     858
 
BbvCI CC'TCA_GC

Cuts at:      0     352     873 
   Size:        352     521
 
BclI T'GATC_A

Cuts at:      0      32     873 
   Size:         32     841
 
BfrBI ATG'CAT

Cuts at:      0     813     873 
   Size:        813      60
 
BlpI GC'TnA_GC

Cuts at:      0     832     873 
   Size:        832      41
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     400     873 
   Size:        400     473
 
Bpu10I CC'TnA_GC

Cuts at:      0     352     873 
   Size:        352     521
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     873 
   Size:         67     806
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     324     873 
   Size:        324     549
 
BsaBI GATnn'nnATC

Cuts at:      0       7     145     873 
   Size:          7     138     728

  Fragments arranged by size: 

                 728     138       7
 
BseYI C'CCAG_C

Cuts at:      0      93     873 
   Size:         93     780
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     873 
   Size:         88     785
 
BsmI GAATG_Cn'

Cuts at:      0     813     873 
   Size:        813      60
 
BspHI T'CATG_A

Cuts at:      0      29     873 
   Size:         29     844
 
Bsu36I CC'TnA_GG

Cuts at:      0     676     873 
   Size:        676     197
 
BtgI C'CryG_G

Cuts at:      0     467     873 
   Size:        467     406
 
BtsI GCAGTG_nn'

Cuts at:      0      95     639     668     873 
   Size:         95     544      29     205

  Fragments arranged by size: 

                 544     205      95      29
 
EarI CTCTTCn'nnn_

Cuts at:      0     327     873 
   Size:        327     546
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     873 
   Size:        413     460
 
EcoO109I rG'GnC_Cy

Cuts at:      0     680     873 
   Size:        680     193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     609     641     873 
   Size:        609      32     232

  Fragments arranged by size: 

                 609     232      32
 
HindIII A'AGCT_T

Cuts at:      0     785     873 
   Size:        785      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     873 
   Size:         36     837
 
MslI CAynn'nnrTG

Cuts at:      0      40     808     873 
   Size:         40     768      65

  Fragments arranged by size: 

                 768      65      40
 
NcoI C'CATG_G

Cuts at:      0     467     873 
   Size:        467     406
 
NsiI A_TGCA'T

Cuts at:      0     815     873 
   Size:        815      58
 
PpuMI rG'GwC_Cy

Cuts at:      0     680     873 
   Size:        680     193
 
SfcI C'TryA_G

Cuts at:      0     175     873 
   Size:        175     698
 
SmlI C'TyrA_G

Cuts at:      0      46     873 
   Size:         46     827
 
StyI C'CwwG_G

Cuts at:      0     467     873 
   Size:        467     406
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     346     873 
   Size:        346     527
 
TatI w'GTAC_w

Cuts at:      0     228     873 
   Size:        228     645
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     594     873 
   Size:        594     279
 
XmnI GAAnn'nnTTC

Cuts at:      0     515     873 
   Size:        515     358
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApoI     BbsI    BbvCI     BclI    BfrBI
     BlpI     BmrI   Bpu10I    BpuEI     BsaI    BsaBI    BseYI     BsgI
     BsmI    BspHI   Bsu36I     BtgI     BtsI     EarI  Eco57MI EcoO109I
    Hin4I  HindIII     MmeI     MslI     NcoI     NsiI    PpuMI     SfcI
     SmlI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BglI    BglII Bme1580I    BmgBI     BmtI     BplI
     BpmI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI