Restriction map for
pF1KE2426
(Linear) (Six-base) MAPSORT of: KIEE2426.seq Check: 9663 from: 1 to: 1851
KIEE2426.orf
With 182 enzymes: SgfI *
November 16, 2012 10:46 ..
AccI GT'mk_AC
Cuts at: 0 581 1629 1851
Size: 581 1048 222
Fragments arranged by size:
1048 581 222
Acc65I G'GTAC_C
Cuts at: 0 1812 1851
Size: 1812 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1838 1851
Size: 844 994 13
Fragments arranged by size:
994 844 13
AflII C'TTAA_G
Cuts at: 0 1614 1851
Size: 1614 237
AflIII A'CryG_T
Cuts at: 0 1771 1851
Size: 1771 80
AhdI GACnn_n'nnGTC
Cuts at: 0 1554 1851
Size: 1554 297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 57 756 1851
Size: 57 699 1095
Fragments arranged by size:
1095 699 57
AlwNI CAG_nnn'CTG
Cuts at: 0 232 601 1851
Size: 232 369 1250
Fragments arranged by size:
1250 369 232
ApaI G_GGCC'C
Cuts at: 0 1667 1851
Size: 1667 184
ApaLI G'TGCA_C
Cuts at: 0 674 1851
Size: 674 1177
ApoI r'AATT_y
Cuts at: 0 443 464 1220 1699 1851
Size: 443 21 756 479 152
Fragments arranged by size:
756 479 443 152 21
AvaI C'yCGr_G
Cuts at: 0 149 1666 1851
Size: 149 1517 185
Fragments arranged by size:
1517 185 149
BanI G'GyrC_C
Cuts at: 0 6 1812 1851
Size: 6 1806 39
Fragments arranged by size:
1806 39 6
BanII G_rGCy'C
Cuts at: 0 242 333 408 1095 1667 1851
Size: 242 91 75 687 572 184
Fragments arranged by size:
687 572 242 184 91 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 437 1851
Size: 92 345 1414
Fragments arranged by size:
1414 345 92
BbvCI CC'TCA_GC
Cuts at: 0 602 1851
Size: 602 1249
BciVI GTATCCnnnnn_n'
Cuts at: 0 653 1529 1851
Size: 653 876 322
Fragments arranged by size:
876 653 322
Bme1580I G_kGCm'C
Cuts at: 0 678 1667 1851
Size: 678 989 184
Fragments arranged by size:
989 678 184
BmrI ACTGGGnnnn_n'
Cuts at: 0 1393 1815 1851
Size: 1393 422 36
Fragments arranged by size:
1393 422 36
BmtI G_CTAG'C
Cuts at: 0 16 1851
Size: 16 1835
Bpu10I CC'TnA_GC
Cuts at: 0 602 1851
Size: 602 1249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1471 1851
Size: 1471 380
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1841 1851
Size: 1841 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1851
Size: 245 1606
BsiHKAI G_wGCw'C
Cuts at: 0 333 678 1095 1851
Size: 333 345 417 756
Fragments arranged by size:
756 417 345 333
BsmI GAATG_Cn'
Cuts at: 0 1043 1851
Size: 1043 808
Bsp1286I G_dGCh'C
Cuts at: 0 242 333 408 678 1095 1667 1851
Size: 242 91 75 270 417 572 184
Fragments arranged by size:
572 417 270 242 184 91 75
BspHI T'CATG_A
Cuts at: 0 615 1584 1851
Size: 615 969 267
Fragments arranged by size:
969 615 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1842 1851
Size: 1842 9
BsrDI GCAATG_nn'
Cuts at: 0 1214 1851
Size: 1214 637
BsrFI r'CCGG_y
Cuts at: 0 1599 1851
Size: 1599 252
BsrGI T'GTAC_A
Cuts at: 0 1758 1851
Size: 1758 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 232 1851
Size: 232 1619
BstYI r'GATC_y
Cuts at: 0 916 1785 1851
Size: 916 869 66
Fragments arranged by size:
916 869 66
BstZ17I GTA'TAC
Cuts at: 0 582 1630 1851
Size: 582 1048 221
Fragments arranged by size:
1048 582 221
BtgI C'CryG_G
Cuts at: 0 744 1374 1447 1851
Size: 744 630 73 404
Fragments arranged by size:
744 630 404 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1086 1121 1851
Size: 1086 35 730
Fragments arranged by size:
1086 730 35
DraI TTT'AAA
Cuts at: 0 372 1851
Size: 372 1479
EaeI y'GGCC_r
Cuts at: 0 1048 1851
Size: 1048 803
EarI CTCTTCn'nnn_
Cuts at: 0 385 1851
Size: 385 1466
EcoICRI GAG'CTC
Cuts at: 0 331 1093 1851
Size: 331 762 758
Fragments arranged by size:
762 758 331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1838 1851
Size: 844 994 13
Fragments arranged by size:
994 844 13
EcoO109I rG'GnC_Cy
Cuts at: 0 229 606 1831 1851
Size: 229 377 1225 20
Fragments arranged by size:
1225 377 229 20
EcoRI G'AATT_C
Cuts at: 0 464 1851
Size: 464 1387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 116 1851
Size: 116 1735
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 315 347 858 890 908 940 1540 1572
Size: 315 32 511 32 18 32 600 32
Cuts at: 1572 1731 1763 1851
Size: 159 32 88
Fragments arranged by size:
600 511 315 159 88 32 32 32
32 32 18
KpnI G_GTAC'C
Cuts at: 0 1816 1851
Size: 1816 35
MslI CAynn'nnrTG
Cuts at: 0 39 295 620 908 935 1207 1329 1589
Size: 39 256 325 288 27 272 122 260
Cuts at: 1589 1851
Size: 262
Fragments arranged by size:
325 288 272 262 260 256 122 39
27
MspA1I CmG'CkG
Cuts at: 0 250 598 1851
Size: 250 348 1253
Fragments arranged by size:
1253 348 250
NaeI GCC'GGC
Cuts at: 0 1601 1851
Size: 1601 250
NcoI C'CATG_G
Cuts at: 0 744 1851
Size: 744 1107
NgoMIV G'CCGG_C
Cuts at: 0 1599 1851
Size: 1599 252
NheI G'CTAG_C
Cuts at: 0 12 1851
Size: 12 1839
NspI r_CATG'y
Cuts at: 0 715 1775 1851
Size: 715 1060 76
Fragments arranged by size:
1060 715 76
PciI A'CATG_T
Cuts at: 0 1771 1851
Size: 1771 80
PpuMI rG'GwC_Cy
Cuts at: 0 606 1831 1851
Size: 606 1225 20
Fragments arranged by size:
1225 606 20
PspOMI G'GGCC_C
Cuts at: 0 1663 1851
Size: 1663 188
PstI C_TGCA'G
Cuts at: 0 237 249 1689 1851
Size: 237 12 1440 162
Fragments arranged by size:
1440 237 162 12
PvuII CAG'CTG
Cuts at: 0 250 598 1851
Size: 250 348 1253
Fragments arranged by size:
1253 348 250
SacI G_AGCT'C
Cuts at: 0 333 1095 1851
Size: 333 762 756
Fragments arranged by size:
762 756 333
SbfI CC_TGCA'GG
Cuts at: 0 237 1689 1851
Size: 237 1452 162
Fragments arranged by size:
1452 237 162
ScaI AGT'ACT
Cuts at: 0 1382 1851
Size: 1382 469
SfcI C'TryA_G
Cuts at: 0 233 245 1685 1851
Size: 233 12 1440 166
Fragments arranged by size:
1440 233 166 12
SmaI CCC'GGG
Cuts at: 0 151 1851
Size: 151 1700
SmlI C'TyrA_G
Cuts at: 0 1486 1614 1851
Size: 1486 128 237
Fragments arranged by size:
1486 237 128
SpeI A'CTAG_T
Cuts at: 0 1255 1851
Size: 1255 596
SspI AAT'ATT
Cuts at: 0 1800 1851
Size: 1800 51
StyI C'CwwG_G
Cuts at: 0 567 744 893 1055 1496 1851
Size: 567 177 149 162 441 355
Fragments arranged by size:
567 441 355 177 162 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1375 1797 1851
Size: 1375 422 54
Fragments arranged by size:
1375 422 54
TatI w'GTAC_w
Cuts at: 0 1380 1758 1851
Size: 1380 378 93
Fragments arranged by size:
1380 378 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 521 553 1851
Size: 521 32 1298
Fragments arranged by size:
1298 521 32
XbaI T'CTAG_A
Cuts at: 0 978 1851
Size: 978 873
XmaI C'CCGG_G
Cuts at: 0 149 1851
Size: 149 1702
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI
BciVI Bme1580I BmrI BmtI Bpu10I BpuEI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BstAPI BstYI BstZ17I BtgI CspCI DraI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I KpnI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PciI PpuMI
PspOMI PstI PvuII SacI SbfI ScaI SfcI SmaI
SmlI SpeI SspI StyI TaqII TatI TstI XbaI
XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AleI AloI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI
BfrBI BglI BglII BlpI BmgBI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI
BsmBI BspEI BsrBI BssHII BssSI BstBI BstEII BstXI
Bsu36I BtgZI BtsI ClaI DraIII DrdI EagI EciI
EcoNI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MmeI MscI NarI NdeI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SexAI SfiI SfoI SgfI
SgrAI SnaBI SphI SrfI StuI SwaI Tth111I XcmI
XhoI XmnI ZraI