Restriction map for
pF1KE2390
(Linear) (Six-base) MAPSORT of: KIEE2390.seq Check: 1898 from: 1 to: 1503
KIEE2390.orf
With 182 enzymes: SgfI *
January 17, 2012 13:59 ..
AccI GT'mk_AC
Cuts at: 0 84 1503
Size: 84 1419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 1503
Size: 1387 116
AleI CACnn'nnGTG
Cuts at: 0 226 499 1503
Size: 226 273 1004
Fragments arranged by size:
1004 273 226
AlwNI CAG_nnn'CTG
Cuts at: 0 449 1503
Size: 449 1054
ApoI r'AATT_y
Cuts at: 0 363 1503
Size: 363 1140
AvaI C'yCGr_G
Cuts at: 0 429 1093 1503
Size: 429 664 410
Fragments arranged by size:
664 429 410
AvrII C'CTAG_G
Cuts at: 0 670 1503
Size: 670 833
BanI G'GyrC_C
Cuts at: 0 42 1503
Size: 42 1461
BanII G_rGCy'C
Cuts at: 0 15 836 1118 1503
Size: 15 821 282 385
Fragments arranged by size:
821 385 282 15
BbeI G_GCGC'C
Cuts at: 0 46 1503
Size: 46 1457
BbsI GAAGACnn'nnnn_
Cuts at: 0 789 1503
Size: 789 714
BfrBI ATG'CAT
Cuts at: 0 767 1503
Size: 767 736
BglI GCCn_nnn'nGGC
Cuts at: 0 645 1503
Size: 645 858
BglII A'GATC_T
Cuts at: 0 1043 1503
Size: 1043 460
BlpI GC'TnA_GC
Cuts at: 0 262 1503
Size: 262 1241
BmgBI CAC'GTC
Cuts at: 0 336 372 1503
Size: 336 36 1131
Fragments arranged by size:
1131 336 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1326 1503
Size: 1326 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 644 1118 1472 1503
Size: 262 382 474 354 31
Fragments arranged by size:
474 382 354 262 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 140 175 892 1094 1503
Size: 140 35 717 202 409
Fragments arranged by size:
717 409 202 140 35
BsaAI yAC'GTr
Cuts at: 0 1472 1503
Size: 1472 31
BsaHI Gr'CG_yC
Cuts at: 0 43 59 1466 1503
Size: 43 16 1407 37
Fragments arranged by size:
1407 43 37 16
BsaWI w'CCGG_w
Cuts at: 0 1175 1290 1503
Size: 1175 115 213
Fragments arranged by size:
1175 213 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 137 167 1503
Size: 137 30 1336
Fragments arranged by size:
1336 137 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1302 1503
Size: 1302 201
BseYI C'CCAG_C
Cuts at: 0 27 36 266 1145 1503
Size: 27 9 230 879 358
Fragments arranged by size:
879 358 230 27 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1503
Size: 979 524
BsiHKAI G_wGCw'C
Cuts at: 0 223 284 1503
Size: 223 61 1219
Fragments arranged by size:
1219 223 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1314 1503
Size: 1314 189
Bsp1286I G_dGCh'C
Cuts at: 0 15 223 284 836 1118 1503
Size: 15 208 61 552 282 385
Fragments arranged by size:
552 385 282 208 61 15
BspEI T'CCGG_A
Cuts at: 0 1175 1290 1503
Size: 1175 115 213
Fragments arranged by size:
1175 213 115
BspHI T'CATG_A
Cuts at: 0 591 1503
Size: 591 912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 812 1001 1079 1503
Size: 812 189 78 424
Fragments arranged by size:
812 424 189 78
BsrBI CCG'CTC
Cuts at: 0 48 1004 1503
Size: 48 956 499
Fragments arranged by size:
956 499 48
BsrDI GCAATG_nn'
Cuts at: 0 755 1503
Size: 755 748
BstAPI GCAn_nnn'nTGC
Cuts at: 0 449 1503
Size: 449 1054
BstEII G'GTnAC_C
Cuts at: 0 462 1503
Size: 462 1041
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1398 1503
Size: 1398 105
BstYI r'GATC_y
Cuts at: 0 1043 1503
Size: 1043 460
BtgI C'CryG_G
Cuts at: 0 63 944 1391 1503
Size: 63 881 447 112
Fragments arranged by size:
881 447 112 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1311 1503
Size: 1311 192
ClaI AT'CG_AT
Cuts at: 0 293 1259 1503
Size: 293 966 244
Fragments arranged by size:
966 293 244
DrdI GACnn_nn'nnGTC
Cuts at: 0 388 1503
Size: 388 1115
EaeI y'GGCC_r
Cuts at: 0 127 615 785 1388 1503
Size: 127 488 170 603 115
Fragments arranged by size:
603 488 170 127 115
EarI CTCTTCn'nnn_
Cuts at: 0 206 742 785 1204 1414 1503
Size: 206 536 43 419 210 89
Fragments arranged by size:
536 419 210 206 89 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 328 712 1503
Size: 91 237 384 791
Fragments arranged by size:
791 384 237 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 644 1118 1387 1472 1503
Size: 262 382 474 269 85 31
Fragments arranged by size:
474 382 269 262 85 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 674 1503
Size: 674 829
EcoO109I rG'GnC_Cy
Cuts at: 0 270 556 1445 1503
Size: 270 286 889 58
Fragments arranged by size:
889 286 270 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 249 753 1503
Size: 249 504 750
Fragments arranged by size:
750 504 249
HaeII r_GCGC'y
Cuts at: 0 37 46 696 1503
Size: 37 9 650 807
Fragments arranged by size:
807 650 37 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 1503
Size: 137 32 1334
Fragments arranged by size:
1334 137 32
HincII GTy'rAC
Cuts at: 0 85 750 1503
Size: 85 665 753
Fragments arranged by size:
753 665 85
KasI G'GCGC_C
Cuts at: 0 42 1503
Size: 42 1461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1503
Size: 428 1075
MscI TGG'CCA
Cuts at: 0 617 787 1390 1503
Size: 617 170 603 113
Fragments arranged by size:
617 603 170 113
MslI CAynn'nnrTG
Cuts at: 0 226 499 590 1396 1503
Size: 226 273 91 806 107
Fragments arranged by size:
806 273 226 107 91
MspA1I CmG'CkG
Cuts at: 0 65 449 1065 1338 1503
Size: 65 384 616 273 165
Fragments arranged by size:
616 384 273 165 65
NarI GG'CG_CC
Cuts at: 0 43 1503
Size: 43 1460
NcoI C'CATG_G
Cuts at: 0 944 1391 1503
Size: 944 447 112
Fragments arranged by size:
944 447 112
NsiI A_TGCA'T
Cuts at: 0 769 1503
Size: 769 734
PasI CC'CwG_GG
Cuts at: 0 7 1503
Size: 7 1496
PflMI CCAn_nnn'nTGG
Cuts at: 0 1141 1503
Size: 1141 362
PfoI T'CCnGG_A
Cuts at: 0 89 1134 1460 1503
Size: 89 1045 326 43
Fragments arranged by size:
1045 326 89 43
PpuMI rG'GwC_Cy
Cuts at: 0 270 556 1445 1503
Size: 270 286 889 58
Fragments arranged by size:
889 286 270 58
PshAI GACnn'nnGTC
Cuts at: 0 1450 1503
Size: 1450 53
PstI C_TGCA'G
Cuts at: 0 454 545 1343 1503
Size: 454 91 798 160
Fragments arranged by size:
798 454 160 91
PvuII CAG'CTG
Cuts at: 0 449 1338 1503
Size: 449 889 165
Fragments arranged by size:
889 449 165
SacII CC_GC'GG
Cuts at: 0 66 1503
Size: 66 1437
SalI G'TCGA_C
Cuts at: 0 83 1503
Size: 83 1420
SanDI GG'GwC_CC
Cuts at: 0 270 1445 1503
Size: 270 1175 58
Fragments arranged by size:
1175 270 58
SexAI A'CCwGG_T
Cuts at: 0 743 1503
Size: 743 760
SfcI C'TryA_G
Cuts at: 0 255 450 513 541 1339 1503
Size: 255 195 63 28 798 164
Fragments arranged by size:
798 255 195 164 63 28
SfoI GGC'GCC
Cuts at: 0 44 1503
Size: 44 1459
SmaI CCC'GGG
Cuts at: 0 431 1503
Size: 431 1072
SspI AAT'ATT
Cuts at: 0 1168 1503
Size: 1168 335
StuI AGG'CCT
Cuts at: 0 551 1503
Size: 551 952
StyI C'CwwG_G
Cuts at: 0 670 944 1391 1503
Size: 670 274 447 112
Fragments arranged by size:
670 447 274 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1030 1503
Size: 1030 473
TatI w'GTAC_w
Cuts at: 0 230 1503
Size: 230 1273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 1503
Size: 307 32 1164
Fragments arranged by size:
1164 307 32
Tth111I GACn'n_nGTC
Cuts at: 0 151 1503
Size: 151 1352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1142 1503
Size: 1142 361
XmaI C'CCGG_G
Cuts at: 0 429 1503
Size: 429 1074
XmnI GAAnn'nnTTC
Cuts at: 0 100 1503
Size: 100 1403
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI AvaI AvrII BanI
BanII BbeI BbsI BfrBI BglI BglII BlpI BmgBI
BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI
ClaI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NarI NcoI NsiI PasI PflMI PfoI PpuMI
PshAI PstI PvuII SacII SalI SanDI SexAI SfcI
SfoI SmaI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbvCI BcgI BciVI BclI Bme1580I BmrI
BmtI Bpu10I BpuEI BsaBI BsiEI BsiWI BsmI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI CspCI
DraI DraIII EagI EcoICRI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NspI PacI PciI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SapI SbfI ScaI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SwaI XbaI XhoI ZraI