Restriction map for
pF1KE2387
(Linear) (Six-base) MAPSORT of: KIEE2387.seq Check: 2679 from: 1 to: 5268
KIEE2387.orf
With 182 enzymes: SgfI *
December 8, 2011 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1149 2139 4967 5268
Size: 1149 990 2828 301
Fragments arranged by size:
2828 1149 990 301
AatII G_ACGT'C
Cuts at: 0 4604 5268
Size: 4604 664
Acc65I G'GTAC_C
Cuts at: 0 1061 5268
Size: 1061 4207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1492 2320 2561 3146 3727 5268
Size: 349 1143 828 241 585 581 1541
Fragments arranged by size:
1541 1143 828 585 581 349 241
AfeI AGC'GCT
Cuts at: 0 661 1314 4286 5268
Size: 661 653 2972 982
Fragments arranged by size:
2972 982 661 653
AflIII A'CryG_T
Cuts at: 0 5048 5268
Size: 5048 220
AgeI A'CCGG_T
Cuts at: 0 1058 5268
Size: 1058 4210
AhdI GACnn_n'nnGTC
Cuts at: 0 5139 5268
Size: 5139 129
AleI CACnn'nnGTG
Cuts at: 0 2500 5268
Size: 2500 2768
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 125 1640 2951 5268
Size: 125 1515 1311 2317
Fragments arranged by size:
2317 1515 1311 125
AlwNI CAG_nnn'CTG
Cuts at: 0 600 633 840 1002 1140 1245 1369 1521
Size: 600 33 207 162 138 105 124 152
Cuts at: 1521 1809 1924 1962 2568 2577 2856 2898 3216
Size: 288 115 38 606 9 279 42 318
Cuts at: 3216 3834 4278 4425 5241 5268
Size: 618 444 147 816 27
Fragments arranged by size:
816 618 606 600 444 318 288 279
207 162 152 147 138 124 115 105
42 38 33 27 9
ApaI G_GGCC'C
Cuts at: 0 539 3015 5268
Size: 539 2476 2253
Fragments arranged by size:
2476 2253 539
ApaLI G'TGCA_C
Cuts at: 0 892 2110 5268
Size: 892 1218 3158
Fragments arranged by size:
3158 1218 892
ApoI r'AATT_y
Cuts at: 0 768 4642 5268
Size: 768 3874 626
Fragments arranged by size:
3874 768 626
AvaI C'yCGr_G
Cuts at: 0 856 2046 3077 4438 4891 5268
Size: 856 1190 1031 1361 453 377
Fragments arranged by size:
1361 1190 1031 856 453 377
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4249 4282 5268
Size: 4249 33 986
Fragments arranged by size:
4249 986 33
BamHI G'GATC_C
Cuts at: 0 396 1580 2826 5268
Size: 396 1184 1246 2442
Fragments arranged by size:
2442 1246 1184 396
BanI G'GyrC_C
Cuts at: 0 755 1061 1164 1741 2820 3073 3610 5268
Size: 755 306 103 577 1079 253 537 1658
Fragments arranged by size:
1658 1079 755 577 537 306 253 103
BanII G_rGCy'C
Cuts at: 0 80 539 1304 2261 3015 4028 4439 4924
Size: 80 459 765 957 754 1013 411 485
Cuts at: 4924 5036 5268
Size: 112 232
Fragments arranged by size:
1013 957 765 754 485 459 411 232
112 80
BbeI G_GCGC'C
Cuts at: 0 759 3077 5268
Size: 759 2318 2191
Fragments arranged by size:
2318 2191 759
BbsI GAAGACnn'nnnn_
Cuts at: 0 986 1480 1693 3457 3905 5268
Size: 986 494 213 1764 448 1363
Fragments arranged by size:
1764 1363 986 494 448 213
BbvCI CC'TCA_GC
Cuts at: 0 1703 1756 2278 4304 5268
Size: 1703 53 522 2026 964
Fragments arranged by size:
2026 1703 964 522 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 200 4087 4121 5268
Size: 166 34 3887 34 1147
Fragments arranged by size:
3887 1147 166 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1633 3082 3121 5268
Size: 1633 1449 39 2147
Fragments arranged by size:
2147 1633 1449 39
BglII A'GATC_T
Cuts at: 0 368 2357 3803 5090 5268
Size: 368 1989 1446 1287 178
Fragments arranged by size:
1989 1446 1287 368 178
BlpI GC'TnA_GC
Cuts at: 0 3271 5268
Size: 3271 1997
Bme1580I G_kGCm'C
Cuts at: 0 539 896 1169 2114 2940 3015 5268
Size: 539 357 273 945 826 75 2253
Fragments arranged by size:
2253 945 826 539 357 273 75
BmgBI CAC'GTC
Cuts at: 0 207 984 1128 5268
Size: 207 777 144 4140
Fragments arranged by size:
4140 777 207 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 420 2243 5091 5268
Size: 420 1823 2848 177
Fragments arranged by size:
2848 1823 420 177
BmtI G_CTAG'C
Cuts at: 0 3440 5268
Size: 3440 1828
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2497 4044 5109 5268
Size: 2497 1547 1065 159
Fragments arranged by size:
2497 1547 1065 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 808 1618 1870 2110 2599 2681 2746
Size: 741 67 810 252 240 489 82 65
Cuts at: 2746 2857 2932 2962 3070 3217 3265 3598 3667
Size: 111 75 30 108 147 48 333 69
Cuts at: 3667 3994 4228 4309 4375 4447 4489 4588 4729
Size: 327 234 81 66 72 42 99 141
Cuts at: 4729 4750 4812 4864 5268
Size: 21 62 52 404
Fragments arranged by size:
810 741 489 404 333 327 252 240
234 147 141 111 108 99 82 81
75 72 69 67 66 65 62 52
48 42 30 21
Bpu10I CC'TnA_GC
Cuts at: 0 194 995 1703 1756 2278 3247 3751 4304
Size: 194 801 708 53 522 969 504 553
Cuts at: 4304 5268
Size: 964
Fragments arranged by size:
969 964 801 708 553 522 504 194
53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1156 1180 1630 2787 2797 3277 3337
Size: 931 225 24 450 1157 10 480 60
Cuts at: 3337 4216 4540 4849 5268
Size: 879 324 309 419
Fragments arranged by size:
1157 931 879 480 450 419 324 309
225 60 24 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 2284 2432 2626 2944 4435 4726 4775 5268
Size: 2284 148 194 318 1491 291 49 493
Fragments arranged by size:
2284 1491 493 318 291 194 148 49
BsaAI yAC'GTr
Cuts at: 0 2223 2463 2976 5268
Size: 2223 240 513 2292
Fragments arranged by size:
2292 2223 513 240
BsaHI Gr'CG_yC
Cuts at: 0 756 3074 3925 4601 5268
Size: 756 2318 851 676 667
Fragments arranged by size:
2318 851 756 676 667
BsaWI w'CCGG_w
Cuts at: 0 779 1058 1409 1781 3626 4448 5268
Size: 779 279 351 372 1845 822 820
Fragments arranged by size:
1845 822 820 779 372 351 279
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2659 2689 3190 3220 3586 3616 3910 3940
Size: 2659 30 501 30 366 30 294 30
Cuts at: 3940 4810 4840 5268
Size: 870 30 428
Fragments arranged by size:
2659 870 501 428 366 294 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 511 814 949 2587 2683 2716 2854
Size: 442 69 303 135 1638 96 33 138
Cuts at: 2854 2890 2920 3058 3085 3277 3280 3499 3502
Size: 36 30 138 27 192 3 219 3
Cuts at: 3502 3631 3772 3931 3964 4033 4036 4090 4093
Size: 129 141 159 33 69 3 54 3
Cuts at: 4093 4225 4585 4588 5101 5268
Size: 132 360 3 513 167
Fragments arranged by size:
1638 513 442 360 303 219 192 167
159 141 138 138 135 132 129 96
69 69 54 36 33 33 30 27
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 745 1295 1315 1432 1871 2118 2372 3191
Size: 745 550 20 117 439 247 254 819
Cuts at: 3191 5268
Size: 2077
Fragments arranged by size:
2077 819 745 550 439 254 247 117
20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 648 802 1153 1636 3529 4072 4448
Size: 70 578 154 351 483 1893 543 376
Cuts at: 4448 5268
Size: 820
Fragments arranged by size:
1893 820 578 543 483 376 351 154
70
BsiEI CG_ry'CG
Cuts at: 0 848 1340 5268
Size: 848 492 3928
Fragments arranged by size:
3928 848 492
BsiHKAI G_wGCw'C
Cuts at: 0 80 200 854 896 1304 1699 1796 2047
Size: 80 120 654 42 408 395 97 251
Cuts at: 2047 2053 2078 2114 2201 2746 2813 3095 3353
Size: 6 25 36 87 545 67 282 258
Cuts at: 3353 4028 4439 4502 5268
Size: 675 411 63 766
Fragments arranged by size:
766 675 654 545 411 408 395 282
258 251 120 97 87 80 67 63
42 36 25 6
BsmI GAATG_Cn'
Cuts at: 0 114 5268
Size: 114 5154
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2951 3932 5268
Size: 2951 981 1336
Fragments arranged by size:
2951 1336 981
Bsp1286I G_dGCh'C
Cuts at: 0 80 200 539 854 896 1169 1304 1699
Size: 80 120 339 315 42 273 135 395
Cuts at: 1699 1796 2047 2053 2078 2114 2201 2261 2746
Size: 97 251 6 25 36 87 60 485
Cuts at: 2746 2813 2940 3015 3095 3353 4028 4439 4502
Size: 67 127 75 80 258 675 411 63
Cuts at: 4502 4924 5036 5268
Size: 422 112 232
Fragments arranged by size:
675 485 422 411 395 339 315 273
258 251 232 135 127 120 112 97
87 80 80 75 67 63 60 42
36 25 6
BspEI T'CCGG_A
Cuts at: 0 3626 4448 5268
Size: 3626 822 820
Fragments arranged by size:
3626 822 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 479 929 1149 2139 2147 2324 4730
Size: 356 123 450 220 990 8 177 2406
Cuts at: 4730 4793 4967 5268
Size: 63 174 301
Fragments arranged by size:
2406 990 450 356 301 220 177 174
123 63 8
BsrBI CCG'CTC
Cuts at: 0 1194 1632 2712 3312 4200 4227 5268
Size: 1194 438 1080 600 888 27 1041
Fragments arranged by size:
1194 1080 1041 888 600 438 27
BsrDI GCAATG_nn'
Cuts at: 0 269 5268
Size: 269 4999
BsrFI r'CCGG_y
Cuts at: 0 752 1058 1556 4181 4412 4613 5268
Size: 752 306 498 2625 231 201 655
Fragments arranged by size:
2625 752 655 498 306 231 201
BssSI C'ACGA_G
Cuts at: 0 1891 4778 5268
Size: 1891 2887 490
Fragments arranged by size:
2887 1891 490
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1140 1164 1800 2025 2524 4380 5268
Size: 1140 24 636 225 499 1856 888
Fragments arranged by size:
1856 1140 888 636 499 225 24
BstEII G'GTnAC_C
Cuts at: 0 4320 5268
Size: 4320 948
BstXI CCAn_nnnn'nTGG
Cuts at: 0 460 1039 1209 1551 1848 3504 3870 5268
Size: 460 579 170 342 297 1656 366 1398
Fragments arranged by size:
1656 1398 579 460 366 342 297 170
BstYI r'GATC_y
Cuts at: 0 368 396 1580 2357 2826 3803 3881 3969
Size: 368 28 1184 777 469 977 78 88
Cuts at: 3969 4544 5090 5268
Size: 575 546 178
Fragments arranged by size:
1184 977 777 575 546 469 368 178
88 78 28
Bsu36I CC'TnA_GG
Cuts at: 0 1370 2783 3655 5268
Size: 1370 1413 872 1613
Fragments arranged by size:
1613 1413 1370 872
BtgI C'CryG_G
Cuts at: 0 1208 1970 2208 3317 4799 4925 5268
Size: 1208 762 238 1109 1482 126 343
Fragments arranged by size:
1482 1208 1109 762 343 238 126
BtsI GCAGTG_nn'
Cuts at: 0 1743 2047 2076 2917 5268
Size: 1743 304 29 841 2351
Fragments arranged by size:
2351 1743 841 304 29
ClaI AT'CG_AT
Cuts at: 0 4124 5268
Size: 4124 1144
DraIII CAC_nnn'GTG
Cuts at: 0 492 1222 5268
Size: 492 730 4046
Fragments arranged by size:
4046 730 492
DrdI GACnn_nn'nnGTC
Cuts at: 0 5212 5268
Size: 5212 56
EaeI y'GGCC_r
Cuts at: 0 266 545 845 1337 1541 1588 1838 1883
Size: 266 279 300 492 204 47 250 45
Cuts at: 1883 1967 2033 2057 2211 3113 3320 3371 3535
Size: 84 66 24 154 902 207 51 164
Cuts at: 3535 4928 5268
Size: 1393 340
Fragments arranged by size:
1393 902 492 340 300 279 266 250
207 204 164 154 84 66 51 47
45 24
EagI C'GGCC_G
Cuts at: 0 845 1337 5268
Size: 845 492 3931
Fragments arranged by size:
3931 845 492
EarI CTCTTCn'nnn_
Cuts at: 0 13 16 1849 2435 2504 3122 3127 3992
Size: 13 3 1833 586 69 618 5 865
Cuts at: 3992 4397 4682 5159 5268
Size: 405 285 477 109
Fragments arranged by size:
1833 865 618 586 477 405 285 109
69 13 5 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 274 310 3034 3562 3565 4492 4819 5268
Size: 274 36 2724 528 3 927 327 449
Fragments arranged by size:
2724 927 528 449 327 274 36 3
EcoICRI GAG'CTC
Cuts at: 0 78 1302 4026 4437 5268
Size: 78 1224 2724 411 831
Fragments arranged by size:
2724 1224 831 411 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 741 808 1492 1618 1870 2110 2320
Size: 349 392 67 684 126 252 240 210
Cuts at: 2320 2561 2599 2681 2746 2857 2932 2962 3070
Size: 241 38 82 65 111 75 30 108
Cuts at: 3070 3146 3217 3265 3598 3667 3727 3994 4228
Size: 76 71 48 333 69 60 267 234
Cuts at: 4228 4309 4375 4447 4489 4588 4729 4750 4812
Size: 81 66 72 42 99 141 21 62
Cuts at: 4812 4864 5268
Size: 52 404
Fragments arranged by size:
684 404 392 349 333 267 252 241
240 234 210 141 126 111 108 99
82 81 76 75 72 71 69 67
66 65 62 60 52 48 42 38
30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 217 547 2008 2944 4570 4991 5268
Size: 217 330 1461 936 1626 421 277
Fragments arranged by size:
1626 1461 936 421 330 277 217
EcoO109I rG'GnC_Cy
Cuts at: 0 536 1242 1374 1641 1725 1760 2779 2988
Size: 536 706 132 267 84 35 1019 209
Cuts at: 2988 3011 3651 3667 3831 4353 5104 5169 5268
Size: 23 640 16 164 522 751 65 99
Fragments arranged by size:
1019 751 706 640 536 522 267 209
164 132 99 84 65 35 23 16
EcoRI G'AATT_C
Cuts at: 0 4642 5268
Size: 4642 626
EcoRV GAT'ATC
Cuts at: 0 318 5268
Size: 318 4950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2539 4645 5268
Size: 2539 2106 623
Fragments arranged by size:
2539 2106 623
FspI TGC'GCA
Cuts at: 0 1009 2995 5268
Size: 1009 1986 2273
Fragments arranged by size:
2273 1986 1009
HaeII r_GCGC'y
Cuts at: 0 663 759 1316 2097 2183 3077 3237 4182
Size: 663 96 557 781 86 894 160 945
Cuts at: 4182 4288 4917 4968 5268
Size: 106 629 51 300
Fragments arranged by size:
945 894 781 663 629 557 300 160
106 96 86 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1467 1499 3910 3942 5125 5157 5181 5213
Size: 1467 32 2411 32 1183 32 24 32
Cuts at: 5213 5268
Size: 55
Fragments arranged by size:
2411 1467 1183 55 32 32 32 32
24
HincII GTy'rAC
Cuts at: 0 408 5217 5268
Size: 408 4809 51
Fragments arranged by size:
4809 408 51
KasI G'GCGC_C
Cuts at: 0 755 3073 5268
Size: 755 2318 2195
Fragments arranged by size:
2318 2195 755
KpnI G_GTAC'C
Cuts at: 0 1065 5268
Size: 1065 4203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 4410 5268
Size: 206 4204 858
Fragments arranged by size:
4204 858 206
MscI TGG'CCA
Cuts at: 0 547 1543 1840 1885 2059 5268
Size: 547 996 297 45 174 3209
Fragments arranged by size:
3209 996 547 297 174 45
MslI CAynn'nnrTG
Cuts at: 0 1207 2500 4126 4798 5053 5268
Size: 1207 1293 1626 672 255 215
Fragments arranged by size:
1626 1293 1207 672 255 215
MspA1I CmG'CkG
Cuts at: 0 99 327 477 837 1432 1458 1518 1806
Size: 99 228 150 360 595 26 60 288
Cuts at: 1806 2210 2724 2856 2862 2895 2925 2931 3036
Size: 404 514 132 6 33 30 6 105
Cuts at: 3036 3195 3216 3747 3813 4206 4263 4275 4395
Size: 159 21 531 66 393 57 12 120
Cuts at: 4395 4707 4728 4947 5268
Size: 312 21 219 321
Fragments arranged by size:
595 531 514 404 393 360 321 312
288 228 219 159 150 132 120 105
99 66 60 57 33 30 26 21
21 12 6 6
NaeI GCC'GGC
Cuts at: 0 754 4183 4615 5268
Size: 754 3429 432 653
Fragments arranged by size:
3429 754 653 432
NarI GG'CG_CC
Cuts at: 0 756 3074 5268
Size: 756 2318 2194
Fragments arranged by size:
2318 2194 756
NcoI C'CATG_G
Cuts at: 0 1970 3317 5268
Size: 1970 1347 1951
Fragments arranged by size:
1970 1951 1347
NgoMIV G'CCGG_C
Cuts at: 0 752 4181 4613 5268
Size: 752 3429 432 655
Fragments arranged by size:
3429 752 655 432
NheI G'CTAG_C
Cuts at: 0 3436 5268
Size: 3436 1832
NspI r_CATG'y
Cuts at: 0 657 711 4378 5052 5268
Size: 657 54 3667 674 216
Fragments arranged by size:
3667 674 657 216 54
PciI A'CATG_T
Cuts at: 0 5048 5268
Size: 5048 220
PflMI CCAn_nnn'nTGG
Cuts at: 0 492 840 1161 1738 2151 4835 5241 5268
Size: 492 348 321 577 413 2684 406 27
Fragments arranged by size:
2684 577 492 413 406 348 321 27
PfoI T'CCnGG_A
Cuts at: 0 1485 1940 2069 3281 3875 4424 5234 5268
Size: 1485 455 129 1212 594 549 810 34
Fragments arranged by size:
1485 1212 810 594 549 455 129 34
PmlI CAC'GTG
Cuts at: 0 2223 2463 5268
Size: 2223 240 2805
Fragments arranged by size:
2805 2223 240
PpuMI rG'GwC_Cy
Cuts at: 0 1641 1760 2988 4353 5104 5268
Size: 1641 119 1228 1365 751 164
Fragments arranged by size:
1641 1365 1228 751 164 119
PshAI GACnn'nnGTC
Cuts at: 0 4836 5268
Size: 4836 432
PspOMI G'GGCC_C
Cuts at: 0 535 3011 5268
Size: 535 2476 2257
Fragments arranged by size:
2476 2257 535
PspXI vC'TCGA_Gb
Cuts at: 0 2046 5268
Size: 2046 3222
PstI C_TGCA'G
Cuts at: 0 104 179 218 647 1145 1277 1454 1805
Size: 104 75 39 429 498 132 177 351
Cuts at: 1805 1865 2231 2573 2861 2903 2924 2930 3104
Size: 60 366 342 288 42 21 6 174
Cuts at: 3104 3182 3221 4178 4349 4727 4910 5177 5268
Size: 78 39 957 171 378 183 267 91
Fragments arranged by size:
957 498 429 378 366 351 342 288
267 183 177 174 171 132 104 91
78 75 60 42 39 39 21 6
PvuII CAG'CTG
Cuts at: 0 99 327 837 1432 1518 1806 2724 2856
Size: 99 228 510 595 86 288 918 132
Cuts at: 2856 2895 2925 2931 3036 3195 3216 3813 4206
Size: 39 30 6 105 159 21 597 393
Cuts at: 4206 4395 4707 4728 5268
Size: 189 312 21 540
Fragments arranged by size:
918 597 595 540 510 393 312 288
228 189 159 132 105 99 86 39
30 21 21 6
SacI G_AGCT'C
Cuts at: 0 80 1304 4028 4439 5268
Size: 80 1224 2724 411 829
Fragments arranged by size:
2724 1224 829 411 80
SacII CC_GC'GG
Cuts at: 0 2211 5268
Size: 2211 3057
SanDI GG'GwC_CC
Cuts at: 0 5104 5268
Size: 5104 164
SapI GCTCTTCn'nnn_
Cuts at: 0 16 1849 3127 3992 4397 5268
Size: 16 1833 1278 865 405 871
Fragments arranged by size:
1833 1278 871 865 405 16
SexAI A'CCwGG_T
Cuts at: 0 116 5268
Size: 116 5152
SfcI C'TryA_G
Cuts at: 0 100 175 214 372 643 1141 1260 1273
Size: 100 75 39 158 271 498 119 13
Cuts at: 1273 1450 1504 1801 1861 2173 2227 2569 2857
Size: 177 54 297 60 312 54 342 288
Cuts at: 2857 2899 2920 2926 3100 3178 3217 4174 4345
Size: 42 21 6 174 78 39 957 171
Cuts at: 4345 4723 4906 5173 5268
Size: 378 183 267 95
Fragments arranged by size:
957 498 378 342 312 297 288 271
267 183 177 174 171 158 119 100
95 78 75 60 54 54 42 39
39 21 13 6
SfoI GGC'GCC
Cuts at: 0 757 3075 5268
Size: 757 2318 2193
Fragments arranged by size:
2318 2193 757
SgrAI Cr'CCGG_yG
Cuts at: 0 752 4181 4412 5268
Size: 752 3429 231 856
Fragments arranged by size:
3429 856 752 231
SmaI CCC'GGG
Cuts at: 0 858 3079 5268
Size: 858 2221 2189
Fragments arranged by size:
2221 2189 858
SmlI C'TyrA_G
Cuts at: 0 946 1171 1195 1645 2046 2766 2812 3292
Size: 946 225 24 450 401 720 46 480
Cuts at: 3292 3352 4195 4438 4555 4864 4891 5268
Size: 60 843 243 117 309 27 377
Fragments arranged by size:
946 843 720 480 450 401 377 309
243 225 117 60 46 27 24
SphI G_CATG'C
Cuts at: 0 711 4378 5268
Size: 711 3667 890
Fragments arranged by size:
3667 890 711
StuI AGG'CCT
Cuts at: 0 4088 5268
Size: 4088 1180
StyI C'CwwG_G
Cuts at: 0 548 698 1637 1970 2183 3317 3425 3479
Size: 548 150 939 333 213 1134 108 54
Cuts at: 3479 3545 3911 4664 4877 5268
Size: 66 366 753 213 391
Fragments arranged by size:
1134 939 753 548 391 366 333 213
213 150 108 66 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 305 1492 1996 2761 5268
Size: 305 1187 504 765 2507
Fragments arranged by size:
2507 1187 765 504 305
TatI w'GTAC_w
Cuts at: 0 677 950 2349 3710 5268
Size: 677 273 1399 1361 1558
Fragments arranged by size:
1558 1399 1361 677 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2659 2691 5268
Size: 2659 32 2577
Fragments arranged by size:
2659 2577 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 407 3918 5238 5268
Size: 407 3511 1320 30
Fragments arranged by size:
3511 1320 407 30
XhoI C'TCGA_G
Cuts at: 0 2046 4438 4891 5268
Size: 2046 2392 453 377
Fragments arranged by size:
2392 2046 453 377
XmaI C'CCGG_G
Cuts at: 0 856 3077 5268
Size: 856 2221 2191
Fragments arranged by size:
2221 2191 856
XmnI GAAnn'nnTTC
Cuts at: 0 5050 5268
Size: 5050 218
ZraI GAC'GTC
Cuts at: 0 4602 5268
Size: 4602 666
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
SexAI SfcI SfoI SgrAI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI AclI AflII AloI AscI AseI AsiSI AvrII
BciVI BclI BfrBI BsaBI BsiWI BspHI BsrGI BssHII
BstBI BstZ17I BtgZI CspCI DraI FseI FspAI HindIII
HpaI MfeI MluI NdeI NotI NruI NsiI PacI
PasI PmeI PpiI PsiI PsrI PvuI RsrII SalI
SbfI ScaI SfiI SgfI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI