Restriction map for
pF1KE2379
(Linear) (Six-base) MAPSORT of: KIEE2379.seq Check: 9369 from: 1 to: 3477
KIEE2379.orf
With 182 enzymes: SgfI *
October 18, 2011 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 202 3477
Size: 202 3275
AatII G_ACGT'C
Cuts at: 0 2381 3477
Size: 2381 1096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1318 1591 1839 2170 2625 3477
Size: 514 804 273 248 331 455 852
Fragments arranged by size:
852 804 514 455 331 273 248
AflIII A'CryG_T
Cuts at: 0 1973 2528 3477
Size: 1973 555 949
Fragments arranged by size:
1973 949 555
AleI CACnn'nnGTG
Cuts at: 0 1870 2305 3477
Size: 1870 435 1172
Fragments arranged by size:
1870 1172 435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 958 3477
Size: 958 2519
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 3477
Size: 1369 32 2076
Fragments arranged by size:
2076 1369 32
AlwNI CAG_nnn'CTG
Cuts at: 0 255 1131 1353 1995 2031 2999 3447 3477
Size: 255 876 222 642 36 968 448 30
Fragments arranged by size:
968 876 642 448 255 222 36 30
ApaI G_GGCC'C
Cuts at: 0 448 680 2259 2472 2836 3372 3432 3477
Size: 448 232 1579 213 364 536 60 45
Fragments arranged by size:
1579 536 448 364 232 213 60 45
ApaLI G'TGCA_C
Cuts at: 0 1662 3477
Size: 1662 1815
AvaI C'yCGr_G
Cuts at: 0 101 524 570 673 752 780 815 2001
Size: 101 423 46 103 79 28 35 1186
Cuts at: 2001 2089 2348 2604 3087 3101 3249 3363 3468
Size: 88 259 256 483 14 148 114 105
Cuts at: 3468 3477
Size: 9
Fragments arranged by size:
1186 483 423 259 256 148 114 105
103 101 88 79 46 35 28 14
9
BanI G'GyrC_C
Cuts at: 0 262 336 565 609 1141 1173 2865 2965
Size: 262 74 229 44 532 32 1692 100
Cuts at: 2965 3477
Size: 512
Fragments arranged by size:
1692 532 512 262 229 100 74 44
32
BanII G_rGCy'C
Cuts at: 0 448 674 680 787 2259 2356 2472 2611
Size: 448 226 6 107 1472 97 116 139
Cuts at: 2611 2791 2836 2915 3035 3372 3432 3477
Size: 180 45 79 120 337 60 45
Fragments arranged by size:
1472 448 337 226 180 139 120 116
107 97 79 60 45 45 6
BbeI G_GCGC'C
Cuts at: 0 266 569 1145 2969 3477
Size: 266 303 576 1824 508
Fragments arranged by size:
1824 576 508 303 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 1260 1579 3477
Size: 1260 319 1898
Fragments arranged by size:
1898 1260 319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 806 840 3477
Size: 806 34 2637
Fragments arranged by size:
2637 806 34
BglI GCCn_nnn'nGGC
Cuts at: 0 574 850 1457 1808 2772 3477
Size: 574 276 607 351 964 705
Fragments arranged by size:
964 705 607 574 351 276
BglII A'GATC_T
Cuts at: 0 1913 3477
Size: 1913 1564
Bme1580I G_kGCm'C
Cuts at: 0 341 448 680 1666 2008 2259 2472 2836
Size: 341 107 232 986 342 251 213 364
Cuts at: 2836 2870 3372 3432 3477
Size: 34 502 60 45
Fragments arranged by size:
986 502 364 342 341 251 232 213
107 60 45 34
BmgBI CAC'GTC
Cuts at: 0 21 591 1149 3111 3477
Size: 21 570 558 1962 366
Fragments arranged by size:
1962 570 558 366 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 3330 3477
Size: 3330 147
BmtI G_CTAG'C
Cuts at: 0 1693 1961 3477
Size: 1693 268 1516
Fragments arranged by size:
1693 1516 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 2536 2587 2790 2832 2859 3066 3118
Size: 771 1765 51 203 42 27 207 52
Cuts at: 3118 3175 3477
Size: 57 302
Fragments arranged by size:
1765 771 302 207 203 57 52 51
42 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 3477
Size: 1012 2465
BsaI GGTCTCn'nnnn_
Cuts at: 0 3151 3477
Size: 3151 326
BsaAI yAC'GTr
Cuts at: 0 663 3477
Size: 663 2814
BsaHI Gr'CG_yC
Cuts at: 0 263 566 601 776 1142 2378 2966 3402
Size: 263 303 35 175 366 1236 588 436
Cuts at: 3402 3477
Size: 75
Fragments arranged by size:
1236 588 436 366 303 263 175 75
35
BsaWI w'CCGG_w
Cuts at: 0 296 3477
Size: 296 3181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2329 2359 2776 2806 3477
Size: 2329 30 417 30 671
Fragments arranged by size:
2329 671 417 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 700 1453 1567 2107 3055 3367 3477
Size: 283 417 753 114 540 948 312 110
Fragments arranged by size:
948 753 540 417 312 283 114 110
BseYI C'CCAG_C
Cuts at: 0 258 613 2314 3058 3423 3440 3477
Size: 258 355 1701 744 365 17 37
Fragments arranged by size:
1701 744 365 355 258 37 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 190 1741 2173 3191 3433 3477
Size: 174 16 1551 432 1018 242 44
Fragments arranged by size:
1551 1018 432 242 174 44 16
BsiEI CG_ry'CG
Cuts at: 0 544 854 1461 1643 2767 3477
Size: 544 310 607 182 1124 710
Fragments arranged by size:
1124 710 607 544 310 182
BsiHKAI G_wGCw'C
Cuts at: 0 1666 3035 3477
Size: 1666 1369 442
Fragments arranged by size:
1666 1369 442
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1297 1893 3477
Size: 1297 596 1584
Fragments arranged by size:
1584 1297 596
Bsp1286I G_dGCh'C
Cuts at: 0 341 448 674 680 787 1666 2008 2259
Size: 341 107 226 6 107 879 342 251
Cuts at: 2259 2356 2472 2611 2791 2836 2870 2915 3035
Size: 97 116 139 180 45 34 45 120
Cuts at: 3035 3372 3432 3477
Size: 337 60 45
Fragments arranged by size:
879 342 341 337 251 226 180 139
120 116 107 107 97 60 45 45
45 34 6
BspHI T'CATG_A
Cuts at: 0 426 3477
Size: 426 3051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 1535 2330 2519 2591 3477
Size: 202 1333 795 189 72 886
Fragments arranged by size:
1333 886 795 202 189 72
BsrBI CCG'CTC
Cuts at: 0 690 2201 3477
Size: 690 1511 1276
Fragments arranged by size:
1511 1276 690
BsrFI r'CCGG_y
Cuts at: 0 3 2762 3477
Size: 3 2759 715
Fragments arranged by size:
2759 715 3
BssHII G'CGCG_C
Cuts at: 0 728 873 3477
Size: 728 145 2604
Fragments arranged by size:
2604 728 145
BssSI C'ACGA_G
Cuts at: 0 970 3477
Size: 970 2507
BstAPI GCAn_nnn'nTGC
Cuts at: 0 255 930 3477
Size: 255 675 2547
Fragments arranged by size:
2547 675 255
BstEII G'GTnAC_C
Cuts at: 0 1119 3477
Size: 1119 2358
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 3477
Size: 462 3015
BstYI r'GATC_y
Cuts at: 0 1913 2063 2363 2495 3477
Size: 1913 150 300 132 982
Fragments arranged by size:
1913 982 300 150 132
BtgI C'CryG_G
Cuts at: 0 578 605 650 680 2776 3477
Size: 578 27 45 30 2096 701
Fragments arranged by size:
2096 701 578 45 30 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 75 731 1449 1662 3477
Size: 75 656 718 213 1815
Fragments arranged by size:
1815 718 656 213 75
BtsI GCAGTG_nn'
Cuts at: 0 253 928 2203 2214 2826 3477
Size: 253 675 1275 11 612 651
Fragments arranged by size:
1275 675 651 612 253 11
EaeI y'GGCC_r
Cuts at: 0 735 851 1458 1508 1640 1979 2261 2387
Size: 735 116 607 50 132 339 282 126
Cuts at: 2387 2764 3179 3477
Size: 377 415 298
Fragments arranged by size:
735 607 415 377 339 298 282 132
126 116 50
EagI C'GGCC_G
Cuts at: 0 851 1458 1640 2764 3477
Size: 851 607 182 1124 713
Fragments arranged by size:
1124 851 713 607 182
EarI CTCTTCn'nnn_
Cuts at: 0 2929 3477
Size: 2929 548
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 478 707 2848 3477
Size: 478 229 2141 629
Fragments arranged by size:
2141 629 478 229
EcoICRI GAG'CTC
Cuts at: 0 3033 3477
Size: 3033 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 771 1318 1591 1839 2170 2536 2587
Size: 514 257 547 273 248 331 366 51
Cuts at: 2587 2625 2790 2832 2859 3066 3118 3175 3477
Size: 38 165 42 27 207 52 57 302
Fragments arranged by size:
547 514 366 331 302 273 257 248
207 165 57 52 51 42 38 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1297 2515 2673 2920 3477
Size: 1297 1218 158 247 557
Fragments arranged by size:
1297 1218 557 247 158
EcoO109I rG'GnC_Cy
Cuts at: 0 416 444 445 706 1128 2256 2319 2469
Size: 416 28 1 261 422 1128 63 150
Cuts at: 2469 2832 2885 3253 3368 3369 3391 3428 3429
Size: 363 53 368 115 1 22 37 1
Cuts at: 3429 3477
Size: 48
Fragments arranged by size:
1128 422 416 368 363 261 150 115
63 53 48 37 28 22 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2685 3477
Size: 2685 792
FseI GG_CCGG'CC
Cuts at: 0 2766 3477
Size: 2766 711
FspI TGC'GCA
Cuts at: 0 934 3477
Size: 934 2543
HaeII r_GCGC'y
Cuts at: 0 228 266 517 569 1145 1843 2088 2969
Size: 228 38 251 52 576 698 245 881
Cuts at: 2969 3141 3477
Size: 172 336
Fragments arranged by size:
881 698 576 336 251 245 228 172
52 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 2055 2087 2776 2808 3477
Size: 1609 32 414 32 689 32 669
Fragments arranged by size:
1609 689 669 414 32 32 32
KasI G'GCGC_C
Cuts at: 0 262 565 1141 2965 3477
Size: 262 303 576 1824 512
Fragments arranged by size:
1824 576 512 303 262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 2570 3008 3477
Size: 989 1581 438 469
Fragments arranged by size:
1581 989 469 438
MscI TGG'CCA
Cuts at: 0 2263 2389 3181 3477
Size: 2263 126 792 296
Fragments arranged by size:
2263 792 296 126
MslI CAynn'nnrTG
Cuts at: 0 1501 1870 2026 2134 2305 3477
Size: 1501 369 156 108 171 1172
Fragments arranged by size:
1501 1172 369 171 156 108
MspA1I CmG'CkG
Cuts at: 0 458 607 682 791 938 1190 1353 1799
Size: 458 149 75 109 147 252 163 446
Cuts at: 1799 1992 3195 3279 3423 3477
Size: 193 1203 84 144 54
Fragments arranged by size:
1203 458 446 252 193 163 149 147
144 109 84 75 54
NaeI GCC'GGC
Cuts at: 0 2764 3477
Size: 2764 713
NarI GG'CG_CC
Cuts at: 0 263 566 1142 2966 3477
Size: 263 303 576 1824 511
Fragments arranged by size:
1824 576 511 303 263
NcoI C'CATG_G
Cuts at: 0 650 3477
Size: 650 2827
NgoMIV G'CCGG_C
Cuts at: 0 2762 3477
Size: 2762 715
NheI G'CTAG_C
Cuts at: 0 1689 1957 3477
Size: 1689 268 1520
Fragments arranged by size:
1689 1520 268
NspI r_CATG'y
Cuts at: 0 2287 2314 2532 3477
Size: 2287 27 218 945
Fragments arranged by size:
2287 945 218 27
PasI CC'CwG_GG
Cuts at: 0 1137 3477
Size: 1137 2340
PciI A'CATG_T
Cuts at: 0 2528 3477
Size: 2528 949
PflMI CCAn_nnn'nTGG
Cuts at: 0 458 1353 3342 3477
Size: 458 895 1989 135
Fragments arranged by size:
1989 895 458 135
PfoI T'CCnGG_A
Cuts at: 0 41 3477
Size: 41 3436
PmlI CAC'GTG
Cuts at: 0 663 3477
Size: 663 2814
PpuMI rG'GwC_Cy
Cuts at: 0 416 706 1128 2319 3477
Size: 416 290 422 1191 1158
Fragments arranged by size:
1191 1158 422 416 290
PshAI GACnn'nnGTC
Cuts at: 0 3217 3477
Size: 3217 260
PspOMI G'GGCC_C
Cuts at: 0 444 676 2255 2468 2832 3368 3428 3477
Size: 444 232 1579 213 364 536 60 49
Fragments arranged by size:
1579 536 444 364 232 213 60 49
PspXI vC'TCGA_Gb
Cuts at: 0 2089 3477
Size: 2089 1388
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2524 2556 3477
Size: 2524 32 921
Fragments arranged by size:
2524 921 32
PstI C_TGCA'G
Cuts at: 0 796 1172 2174 2213 3194 3477
Size: 796 376 1002 39 981 283
Fragments arranged by size:
1002 981 796 376 283 39
PvuII CAG'CTG
Cuts at: 0 458 791 3195 3423 3477
Size: 458 333 2404 228 54
Fragments arranged by size:
2404 458 333 228 54
SacI G_AGCT'C
Cuts at: 0 3035 3477
Size: 3035 442
SacII CC_GC'GG
Cuts at: 0 608 683 3477
Size: 608 75 2794
Fragments arranged by size:
2794 608 75
SanDI GG'GwC_CC
Cuts at: 0 416 706 3477
Size: 416 290 2771
Fragments arranged by size:
2771 416 290
SapI GCTCTTCn'nnn_
Cuts at: 0 2929 3477
Size: 2929 548
SbfI CC_TGCA'GG
Cuts at: 0 2174 3477
Size: 2174 1303
ScaI AGT'ACT
Cuts at: 0 2098 3477
Size: 2098 1379
SfcI C'TryA_G
Cuts at: 0 792 1168 2170 2209 3190 3199 3231 3477
Size: 792 376 1002 39 981 9 32 246
Fragments arranged by size:
1002 981 792 376 246 39 32 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1808 2772 3477
Size: 1808 964 705
Fragments arranged by size:
1808 964 705
SfoI GGC'GCC
Cuts at: 0 264 567 1143 2967 3477
Size: 264 303 576 1824 510
Fragments arranged by size:
1824 576 510 303 264
SmaI CCC'GGG
Cuts at: 0 526 572 754 2350 2606 3089 3103 3251
Size: 526 46 182 1596 256 483 14 148
Cuts at: 3251 3365 3470 3477
Size: 114 105 7
Fragments arranged by size:
1596 526 483 256 182 148 114 105
46 14 7
SmlI C'TyrA_G
Cuts at: 0 1027 2089 3477
Size: 1027 1062 1388
Fragments arranged by size:
1388 1062 1027
SphI G_CATG'C
Cuts at: 0 2314 3477
Size: 2314 1163
StuI AGG'CCT
Cuts at: 0 2442 3477
Size: 2442 1035
StyI C'CwwG_G
Cuts at: 0 650 2240 2720 3311 3477
Size: 650 1590 480 591 166
Fragments arranged by size:
1590 650 591 480 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 627 941 1138 3477
Size: 627 314 197 2339
Fragments arranged by size:
2339 627 314 197
TatI w'GTAC_w
Cuts at: 0 1160 1997 2096 2336 3477
Size: 1160 837 99 240 1141
Fragments arranged by size:
1160 1141 837 240 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1166 1198 2098 2130 2542 2574 3477
Size: 1166 32 900 32 412 32 903
Fragments arranged by size:
1166 903 900 412 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2380 3477
Size: 2380 1097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 459 795 1440 1716 3309 3477
Size: 102 357 336 645 276 1593 168
Fragments arranged by size:
1593 645 357 336 276 168 102
XhoI C'TCGA_G
Cuts at: 0 2089 3477
Size: 2089 1388
XmaI C'CCGG_G
Cuts at: 0 524 570 752 2348 2604 3087 3101 3249
Size: 524 46 182 1596 256 483 14 148
Cuts at: 3249 3363 3468 3477
Size: 114 105 9
Fragments arranged by size:
1596 524 483 256 182 148 114 105
46 14 9
XmnI GAAnn'nnTTC
Cuts at: 0 37 1915 2155 3477
Size: 37 1878 240 1322
Fragments arranged by size:
1878 1322 240 37
ZraI GAC'GTC
Cuts at: 0 2379 3477
Size: 2379 1098
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI
BglI BglII Bme1580I BmgBI BmrI BmtI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI
SapI SbfI ScaI SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BlpI BplI Bpu10I BsaBI BsiWI BsmI
BspEI BsrDI BsrGI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FspAI HincII HindIII
HpaI KpnI MfeI MluI NdeI NotI NruI NsiI
PacI PmeI PpiI PsiI PvuI RsrII SalI SexAI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI