(Linear) (Six-base) MAPSORT of: KIEE2370.seq  Check: 4429  from: 1  to: 519



KIEE2370.orf

 With 182 enzymes: SgfI * 

                        August 18, 2011 15:44  ..

ApaI G_GGCC'C

Cuts at:      0      47     519 
   Size:         47     472
 
BanII G_rGCy'C

Cuts at:      0      47     519 
   Size:         47     472
 
Bme1580I G_kGCm'C

Cuts at:      0      47     519 
   Size:         47     472
 
BmgBI CAC'GTC

Cuts at:      0       6     519 
   Size:          6     513
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     492     519 
   Size:        492      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     505     519 
   Size:        505      14
 
BseYI C'CCAG_C

Cuts at:      0      55     519 
   Size:         55     464
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     519 
   Size:        286     233
 
BsiEI CG_ry'CG

Cuts at:      0     201     519 
   Size:        201     318
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     519 
   Size:         47     472
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     113     519 
   Size:        113     406
 
BsrFI r'CCGG_y

Cuts at:      0      59     519 
   Size:         59     460
 
BstYI r'GATC_y

Cuts at:      0     284     434     519 
   Size:        284     150      85

  Fragments arranged by size: 

                 284     150      85
 
BtgI C'CryG_G

Cuts at:      0     288     318     519 
   Size:        288      30     201

  Fragments arranged by size: 

                 288     201      30
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     211     519 
   Size:        211     308
 
EarI CTCTTCn'nnn_

Cuts at:      0     160     519 
   Size:        160     359
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43      44      87     249     519 
   Size:         43       1      43     162     270

  Fragments arranged by size: 

                 270     162      43      43       1
 
HaeII r_GCGC'y

Cuts at:      0     309     519 
   Size:        309     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     178     210     519 
   Size:        178      32     309

  Fragments arranged by size: 

                 309     178      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     389     519 
   Size:        221     168     130

  Fragments arranged by size: 

                 221     168     130
 
MslI CAynn'nnrTG

Cuts at:      0     478     519 
   Size:        478      41
 
NaeI GCC'GGC

Cuts at:      0      61     519 
   Size:         61     458
 
NgoMIV G'CCGG_C

Cuts at:      0      59     519 
   Size:         59     460
 
NspI r_CATG'y

Cuts at:      0     414     519 
   Size:        414     105
 
PasI CC'CwG_GG

Cuts at:      0      39     519 
   Size:         39     480
 
PpuMI rG'GwC_Cy

Cuts at:      0     249     519 
   Size:        249     270
 
PspOMI G'GGCC_C

Cuts at:      0      43     519 
   Size:         43     476
 
SphI G_CATG'C

Cuts at:      0     414     519 
   Size:        414     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     472     519 
   Size:        472      47
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     492     519 
   Size:        492      27
 
XmnI GAAnn'nnTTC

Cuts at:      0     235     519 
   Size:        235     284
 

 Enzymes that do cut:

     ApaI    BanII Bme1580I    BmgBI    BsaXI    BseRI    BseYI     BsgI
    BsiEI Bsp1286I    BspMI    BsrFI    BstYI     BtgI     DrdI     EarI
 EcoO109I    HaeII    Hin4I     MmeI     MslI     NaeI   NgoMIV     NspI
     PasI    PpuMI   PspOMI     SphI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI