Restriction map for
pF1KE2357
(Linear) (Six-base) MAPSORT of: KIEE2357.seq Check: 8392 from: 1 to: 6945
KIEE2357.orf
With 182 enzymes: SgfI *
August 9, 2016 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4741 6945
Size: 4741 2204
AccI GT'mk_AC
Cuts at: 0 1217 2231 2255 6945
Size: 1217 1014 24 4690
Fragments arranged by size:
4690 1217 1014 24
Acc65I G'GTAC_C
Cuts at: 0 3846 4660 6945
Size: 3846 814 2285
Fragments arranged by size:
3846 2285 814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1292 1586 1742 1769 2463 2522 2642
Size: 1141 151 294 156 27 694 59 120
Cuts at: 2642 2747 3554 4463 5174 5444 6479 6945
Size: 105 807 909 711 270 1035 466
Fragments arranged by size:
1141 1035 909 807 711 694 466 294
270 156 151 120 105 59 27
AflII C'TTAA_G
Cuts at: 0 229 6945
Size: 229 6716
AflIII A'CryG_T
Cuts at: 0 163 2043 3218 5664 6945
Size: 163 1880 1175 2446 1281
Fragments arranged by size:
2446 1880 1281 1175 163
AgeI A'CCGG_T
Cuts at: 0 2250 6945
Size: 2250 4695
AhdI GACnn_n'nnGTC
Cuts at: 0 1006 5668 6945
Size: 1006 4662 1277
Fragments arranged by size:
4662 1277 1006
AleI CACnn'nnGTG
Cuts at: 0 5798 6945
Size: 5798 1147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3226 3950 5600 6945
Size: 3226 724 1650 1345
Fragments arranged by size:
3226 1650 1345 724
AlwNI CAG_nnn'CTG
Cuts at: 0 1876 2587 2770 6087 6945
Size: 1876 711 183 3317 858
Fragments arranged by size:
3317 1876 858 711 183
ApaLI G'TGCA_C
Cuts at: 0 2447 6945
Size: 2447 4498
ApoI r'AATT_y
Cuts at: 0 241 442 567 1084 1462 1500 2671 3010
Size: 241 201 125 517 378 38 1171 339
Cuts at: 3010 3076 3164 3681 4004 4437 5158 5604 6271
Size: 66 88 517 323 433 721 446 667
Cuts at: 6271 6391 6738 6945
Size: 120 347 207
Fragments arranged by size:
1171 721 667 517 517 446 433 378
347 339 323 241 207 201 125 120
88 66 38
AseI AT'TA_AT
Cuts at: 0 311 1261 6945
Size: 311 950 5684
Fragments arranged by size:
5684 950 311
AvaI C'yCGr_G
Cuts at: 0 1004 6945
Size: 1004 5941
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3704 3737 4064 4097 4451 4484 5951 5984
Size: 3704 33 327 33 354 33 1467 33
Cuts at: 5984 6945
Size: 961
Fragments arranged by size:
3704 1467 961 354 327 33 33 33
33
BamHI G'GATC_C
Cuts at: 0 1932 4739 6945
Size: 1932 2807 2206
Fragments arranged by size:
2807 2206 1932
BanI G'GyrC_C
Cuts at: 0 2559 3846 4660 6945
Size: 2559 1287 814 2285
Fragments arranged by size:
2559 2285 1287 814
BanII G_rGCy'C
Cuts at: 0 2612 2806 5641 6945
Size: 2612 194 2835 1304
Fragments arranged by size:
2835 2612 1304 194
BbsI GAAGACnn'nnnn_
Cuts at: 0 2068 2426 2912 5658 5940 6945
Size: 2068 358 486 2746 282 1005
Fragments arranged by size:
2746 2068 1005 486 358 282
BbvCI CC'TCA_GC
Cuts at: 0 3516 4883 6838 6945
Size: 3516 1367 1955 107
Fragments arranged by size:
3516 1955 1367 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5242 5276 6945
Size: 5242 34 1669
Fragments arranged by size:
5242 1669 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 64 4479 6945
Size: 64 4415 2466
Fragments arranged by size:
4415 2466 64
BclI T'GATC_A
Cuts at: 0 3333 4089 5286 5511 6770 6945
Size: 3333 756 1197 225 1259 175
Fragments arranged by size:
3333 1259 1197 756 225 175
BfrBI ATG'CAT
Cuts at: 0 2751 6945
Size: 2751 4194
BglII A'GATC_T
Cuts at: 0 204 1639 2118 6945
Size: 204 1435 479 4827
Fragments arranged by size:
4827 1435 479 204
Bme1580I G_kGCm'C
Cuts at: 0 2451 2564 3608 6945
Size: 2451 113 1044 3337
Fragments arranged by size:
3337 2451 1044 113
BmgBI CAC'GTC
Cuts at: 0 5665 6945
Size: 5665 1280
BmrI ACTGGGnnnn_n'
Cuts at: 0 302 396 2083 2396 3602 5651 6945
Size: 302 94 1687 313 1206 2049 1294
Fragments arranged by size:
2049 1687 1294 1206 313 302 94
BmtI G_CTAG'C
Cuts at: 0 5308 6945
Size: 5308 1637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1749 1763 2005 2178 3011 5004 5454 5825
Size: 1749 14 242 173 833 1993 450 371
Cuts at: 5825 6339 6945
Size: 514 606
Fragments arranged by size:
1993 1749 833 606 514 450 371 242
173 14
Bpu10I CC'TnA_GC
Cuts at: 0 3516 4883 6088 6838 6945
Size: 3516 1367 1205 750 107
Fragments arranged by size:
3516 1367 1205 750 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 2702 3380 6945
Size: 472 2230 678 3565
Fragments arranged by size:
3565 2230 678 472
BsaI GGTCTCn'nnnn_
Cuts at: 0 2217 3505 6945
Size: 2217 1288 3440
Fragments arranged by size:
3440 2217 1288
BsaAI yAC'GTr
Cuts at: 0 2044 6945
Size: 2044 4901
BsaBI GATnn'nnATC
Cuts at: 0 203 460 1100 6945
Size: 203 257 640 5845
Fragments arranged by size:
5845 640 257 203
BsaWI w'CCGG_w
Cuts at: 0 2250 6945
Size: 2250 4695
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 696 726 5493 5523 6945
Size: 696 30 4767 30 1422
Fragments arranged by size:
4767 1422 696 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 356 701 1303 1720 1723 5554 5950
Size: 28 328 345 602 417 3 3831 396
Cuts at: 5950 6025 6692 6945
Size: 75 667 253
Fragments arranged by size:
3831 667 602 417 396 345 328 253
75 28 3
BseYI C'CCAG_C
Cuts at: 0 2587 2611 2731 6284 6945
Size: 2587 24 120 3553 661
Fragments arranged by size:
3553 2587 661 120 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 3691 4988 5212 6917 6945
Size: 702 2989 1297 224 1705 28
Fragments arranged by size:
2989 1705 1297 702 224 28
BsiHKAI G_wGCw'C
Cuts at: 0 129 2451 4587 6705 6853 6945
Size: 129 2322 2136 2118 148 92
Fragments arranged by size:
2322 2136 2118 148 129 92
BsmI GAATG_Cn'
Cuts at: 0 29 3249 6945
Size: 29 3220 3696
Fragments arranged by size:
3696 3220 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2656 6945
Size: 2656 4289
Bsp1286I G_dGCh'C
Cuts at: 0 129 2451 2564 2612 2806 3608 4587 5641
Size: 129 2322 113 48 194 802 979 1054
Cuts at: 5641 6705 6853 6945
Size: 1064 148 92
Fragments arranged by size:
2322 1064 1054 979 802 194 148 129
113 92 48
BspHI T'CATG_A
Cuts at: 0 918 3336 5471 5955 6663 6945
Size: 918 2418 2135 484 708 282
Fragments arranged by size:
2418 2135 918 708 484 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4741 6945
Size: 4741 2204
BsrDI GCAATG_nn'
Cuts at: 0 6142 6273 6901 6945
Size: 6142 131 628 44
Fragments arranged by size:
6142 628 131 44
BsrFI r'CCGG_y
Cuts at: 0 2250 4228 6945
Size: 2250 1978 2717
Fragments arranged by size:
2717 2250 1978
BsrGI T'GTAC_A
Cuts at: 0 3857 6945
Size: 3857 3088
BssSI C'ACGA_G
Cuts at: 0 5485 6945
Size: 5485 1460
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1780 5314 5758 6945
Size: 1780 3534 444 1187
Fragments arranged by size:
3534 1780 1187 444
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4150 6882 6945
Size: 4150 2732 63
Fragments arranged by size:
4150 2732 63
BstYI r'GATC_y
Cuts at: 0 204 636 1639 1932 2118 2336 4739 6945
Size: 204 432 1003 293 186 218 2403 2206
Fragments arranged by size:
2403 2206 1003 432 293 218 204 186
BstZ17I GTA'TAC
Cuts at: 0 2232 2256 6945
Size: 2232 24 4689
Fragments arranged by size:
4689 2232 24
BtgI C'CryG_G
Cuts at: 0 2216 6743 6945
Size: 2216 4527 202
Fragments arranged by size:
4527 2216 202
BtsI GCAGTG_nn'
Cuts at: 0 2742 5698 5780 5793 5805 6945
Size: 2742 2956 82 13 12 1140
Fragments arranged by size:
2956 2742 1140 82 13 12
ClaI AT'CG_AT
Cuts at: 0 5262 6945
Size: 5262 1683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5468 5503 6945
Size: 5468 35 1442
Fragments arranged by size:
5468 1442 35
DraI TTT'AAA
Cuts at: 0 363 747 1499 1613 6945
Size: 363 384 752 114 5332
Fragments arranged by size:
5332 752 384 363 114
DraIII CAC_nnn'GTG
Cuts at: 0 1590 3843 5693 6945
Size: 1590 2253 1850 1252
Fragments arranged by size:
2253 1850 1590 1252
DrdI GACnn_nn'nnGTC
Cuts at: 0 331 2070 6945
Size: 331 1739 4875
Fragments arranged by size:
4875 1739 331
EaeI y'GGCC_r
Cuts at: 0 3884 6369 6403 6571 6945
Size: 3884 2485 34 168 374
Fragments arranged by size:
3884 2485 374 168 34
EarI CTCTTCn'nnn_
Cuts at: 0 95 905 1289 2279 3003 5563 6857 6945
Size: 95 810 384 990 724 2560 1294 88
Fragments arranged by size:
2560 1294 990 810 724 384 95 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1292 1586 1742 1749 1763 1769 2005
Size: 1141 151 294 156 7 14 6 236
Cuts at: 2005 2178 2463 2522 2642 2747 3011 3554 4463
Size: 173 285 59 120 105 264 543 909
Cuts at: 4463 5004 5174 5444 5454 5825 6339 6479 6945
Size: 541 170 270 10 371 514 140 466
Fragments arranged by size:
1141 909 543 541 514 466 371 294
285 270 264 236 173 170 156 151
140 120 105 59 14 10 7 6
EcoO109I rG'GnC_Cy
Cuts at: 0 2104 2767 3302 5779 6945
Size: 2104 663 535 2477 1166
Fragments arranged by size:
2477 2104 1166 663 535
EcoRI G'AATT_C
Cuts at: 0 1462 4437 6271 6945
Size: 1462 2975 1834 674
Fragments arranged by size:
2975 1834 1462 674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2580 6945
Size: 2580 4365
HaeII r_GCGC'y
Cuts at: 0 1322 3121 5769 6945
Size: 1322 1799 2648 1176
Fragments arranged by size:
2648 1799 1322 1176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 696 728 992 1024 1792 1824
Size: 247 32 417 32 264 32 768 32
Cuts at: 1824 1984 2016 2197 2229 2623 2655 3436 3468
Size: 160 32 181 32 394 32 781 32
Cuts at: 3468 4294 4326 5014 5046 5654 5686 6759 6791
Size: 826 32 688 32 608 32 1073 32
Cuts at: 6791 6856 6888 6945
Size: 65 32 57
Fragments arranged by size:
1073 826 781 768 688 608 417 394
264 247 181 160 65 57 32 32
32 32 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 732 1218 2223 3573 5208 5877 6945
Size: 732 486 1005 1350 1635 669 1068
Fragments arranged by size:
1635 1350 1068 1005 732 669 486
HindIII A'AGCT_T
Cuts at: 0 1732 3985 5384 6945
Size: 1732 2253 1399 1561
Fragments arranged by size:
2253 1732 1561 1399
KpnI G_GTAC'C
Cuts at: 0 3850 4664 6945
Size: 3850 814 2281
Fragments arranged by size:
3850 2281 814
MfeI C'AATT_G
Cuts at: 0 3953 6945
Size: 3953 2992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1037 1977 4081 4871 5067 5080 5258
Size: 184 853 940 2104 790 196 13 178
Cuts at: 5258 5790 6945
Size: 532 1155
Fragments arranged by size:
2104 1155 940 853 790 532 196 184
178 13
MscI TGG'CCA
Cuts at: 0 6371 6405 6573 6945
Size: 6371 34 168 372
Fragments arranged by size:
6371 372 168 34
MslI CAynn'nnrTG
Cuts at: 0 829 3208 3998 4148 4547 5798 6014 6020
Size: 829 2379 790 150 399 1251 216 6
Cuts at: 6020 6175 6451 6945
Size: 155 276 494
Fragments arranged by size:
2379 1251 829 790 494 399 276 216
155 150 6
MspA1I CmG'CkG
Cuts at: 0 338 3601 4132 5422 6945
Size: 338 3263 531 1290 1523
Fragments arranged by size:
3263 1523 1290 531 338
NdeI CA'TA_TG
Cuts at: 0 1171 1649 1854 3752 6945
Size: 1171 478 205 1898 3193
Fragments arranged by size:
3193 1898 1171 478 205
NheI G'CTAG_C
Cuts at: 0 5304 6945
Size: 5304 1641
NsiI A_TGCA'T
Cuts at: 0 2753 6945
Size: 2753 4192
NspI r_CATG'y
Cuts at: 0 167 1224 2386 2572 2755 3222 3294 3705
Size: 167 1057 1162 186 183 467 72 411
Cuts at: 3705 3931 4120 5122 5137 6945
Size: 226 189 1002 15 1808
Fragments arranged by size:
1808 1162 1057 1002 467 411 226 189
186 183 167 72 15
PciI A'CATG_T
Cuts at: 0 163 3218 6945
Size: 163 3055 3727
Fragments arranged by size:
3727 3055 163
PflMI CCAn_nnn'nTGG
Cuts at: 0 1056 2587 3843 5775 6351 6378 6945
Size: 1056 1531 1256 1932 576 27 567
Fragments arranged by size:
1932 1531 1256 1056 576 567 27
PfoI T'CCnGG_A
Cuts at: 0 4770 6042 6945
Size: 4770 1272 903
Fragments arranged by size:
4770 1272 903
PpuMI rG'GwC_Cy
Cuts at: 0 2104 3302 6945
Size: 2104 1198 3643
Fragments arranged by size:
3643 2104 1198
PshAI GACnn'nnGTC
Cuts at: 0 4499 6945
Size: 4499 2446
PsiI TTA'TAA
Cuts at: 0 2699 6496 6613 6622 6945
Size: 2699 3797 117 9 323
Fragments arranged by size:
3797 2699 323 117 9
PspXI vC'TCGA_Gb
Cuts at: 0 1004 6945
Size: 1004 5941
PstI C_TGCA'G
Cuts at: 0 1671 2085 2954 5800 6126 6945
Size: 1671 414 869 2846 326 819
Fragments arranged by size:
2846 1671 869 819 414 326
PvuII CAG'CTG
Cuts at: 0 3601 4132 5422 6945
Size: 3601 531 1290 1523
Fragments arranged by size:
3601 1523 1290 531
SalI G'TCGA_C
Cuts at: 0 1216 6945
Size: 1216 5729
SanDI GG'GwC_CC
Cuts at: 0 2104 3302 6945
Size: 2104 1198 3643
Fragments arranged by size:
3643 2104 1198
ScaI AGT'ACT
Cuts at: 0 3531 5518 5719 6945
Size: 3531 1987 201 1226
Fragments arranged by size:
3531 1987 1226 201
SexAI A'CCwGG_T
Cuts at: 0 6755 6945
Size: 6755 190
SfcI C'TryA_G
Cuts at: 0 75 1667 1970 2081 2286 2950 3068 4103
Size: 75 1592 303 111 205 664 118 1035
Cuts at: 4103 4341 5796 6122 6945
Size: 238 1455 326 823
Fragments arranged by size:
1592 1455 1035 823 664 326 303 238
205 118 111 75
SmlI C'TyrA_G
Cuts at: 0 229 451 1004 2717 3395 6945
Size: 229 222 553 1713 678 3550
Fragments arranged by size:
3550 1713 678 553 229 222
SphI G_CATG'C
Cuts at: 0 2386 2572 2755 6945
Size: 2386 186 183 4190
Fragments arranged by size:
4190 2386 186 183
SspI AAT'ATT
Cuts at: 0 195 3620 6945
Size: 195 3425 3325
Fragments arranged by size:
3425 3325 195
StyI C'CwwG_G
Cuts at: 0 1812 3284 5400 6945
Size: 1812 1472 2116 1545
Fragments arranged by size:
2116 1812 1545 1472
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 1102 2745 2851 4228 4396 5255 6945
Size: 497 605 1643 106 1377 168 859 1690
Fragments arranged by size:
1690 1643 1377 859 605 497 168 106
TatI w'GTAC_w
Cuts at: 0 866 3529 3857 5026 5200 5516 5717 6537
Size: 866 2663 328 1169 174 316 201 820
Cuts at: 6537 6945
Size: 408
Fragments arranged by size:
2663 1169 866 820 408 328 316 201
174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5493 5525 6945
Size: 5493 32 1420
Fragments arranged by size:
5493 1420 32
XbaI T'CTAG_A
Cuts at: 0 877 6945
Size: 877 6068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1057 2223 5664 5800 6945
Size: 1057 1166 3441 136 1145
Fragments arranged by size:
3441 1166 1145 1057 136
XhoI C'TCGA_G
Cuts at: 0 1004 6945
Size: 1004 5941
XmnI GAAnn'nnTTC
Cuts at: 0 914 950 1887 2300 2492 4993 6076 6162
Size: 914 36 937 413 192 2501 1083 86
Cuts at: 6162 6945
Size: 783
Fragments arranged by size:
2501 1083 937 914 783 413 192 86
36
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglII Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI BstZ17I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
KpnI MfeI MmeI MscI MslI MspA1I NdeI NheI
NsiI NspI PciI PflMI PfoI PpuMI PshAI PsiI
PspXI PstI PvuII SalI SanDI ScaI SexAI SfcI
SmlI SphI SspI StyI TaqII TatI TstI XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AloI ApaI AscI AsiSI AvrII
BbeI BglI BlpI BplI BsaHI BsiEI BsiWI BspEI
BsrBI BssHII BstBI BstEII Bsu36I BtgZI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HpaI KasI
MluI NaeI NarI NcoI NgoMIV NotI NruI PacI
PasI PmeI PmlI PpiI PspOMI PsrI PvuI RsrII
SacI SacII SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XmaI
ZraI