Restriction map for
pF1KE2346
(Linear) (Six-base) MAPSORT of: KIEE2346.seq Check: 3517 from: 1 to: 8412
KIEE2346.orf
With 182 enzymes: SgfI *
November 15, 2011 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 625 8412
Size: 625 7787
AatII G_ACGT'C
Cuts at: 0 8120 8412
Size: 8120 292
AccI GT'mk_AC
Cuts at: 0 2783 5405 5523 8288 8412
Size: 2783 2622 118 2765 124
Fragments arranged by size:
2783 2765 2622 124 118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1307 1721 1726 2351 2414 3590 3887
Size: 1085 222 414 5 625 63 1176 297
Cuts at: 3887 4219 4401 5123 8027 8187 8412
Size: 332 182 722 2904 160 225
Fragments arranged by size:
2904 1176 1085 722 625 414 332 297
225 222 182 160 63 5
AfeI AGC'GCT
Cuts at: 0 1468 7167 8412
Size: 1468 5699 1245
Fragments arranged by size:
5699 1468 1245
AflIII A'CryG_T
Cuts at: 0 3201 6736 7515 8412
Size: 3201 3535 779 897
Fragments arranged by size:
3535 3201 897 779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 8293 8412
Size: 8293 119
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6810 6842 8412
Size: 6810 32 1570
Fragments arranged by size:
6810 1570 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1330 3460 3690 4741 6994 7075 7378 8412
Size: 1330 2130 230 1051 2253 81 303 1034
Fragments arranged by size:
2253 2130 1330 1051 1034 303 230 81
ApaLI G'TGCA_C
Cuts at: 0 751 8412
Size: 751 7661
ApoI r'AATT_y
Cuts at: 0 390 2818 2862 3677 4021 4364 4689 5020
Size: 390 2428 44 815 344 343 325 331
Cuts at: 5020 5786 5991 6303 6310 7557 7584 7603 7688
Size: 766 205 312 7 1247 27 19 85
Cuts at: 7688 8070 8412
Size: 382 342
Fragments arranged by size:
2428 1247 815 766 390 382 344 343
342 331 325 312 205 85 44 27
19 7
AseI AT'TA_AT
Cuts at: 0 1615 8412
Size: 1615 6797
AvaI C'yCGr_G
Cuts at: 0 920 8412
Size: 920 7492
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6327 6360 8412
Size: 6327 33 2052
Fragments arranged by size:
6327 2052 33
BanI G'GyrC_C
Cuts at: 0 6343 8277 8412
Size: 6343 1934 135
Fragments arranged by size:
6343 1934 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 1091 2529 6791 7553 8412
Size: 1091 1438 4262 762 859
Fragments arranged by size:
4262 1438 1091 859 762
BbvCI CC'TCA_GC
Cuts at: 0 5528 8412
Size: 5528 2884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5791 5825 8412
Size: 5791 34 2587
Fragments arranged by size:
5791 2587 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 547 5568 8412
Size: 547 5021 2844
Fragments arranged by size:
5021 2844 547
BclI T'GATC_A
Cuts at: 0 7014 8412
Size: 7014 1398
BfrBI ATG'CAT
Cuts at: 0 1417 5151 7350 8412
Size: 1417 3734 2199 1062
Fragments arranged by size:
3734 2199 1417 1062
BglI GCCn_nnn'nGGC
Cuts at: 0 298 4731 8264 8412
Size: 298 4433 3533 148
Fragments arranged by size:
4433 3533 298 148
BglII A'GATC_T
Cuts at: 0 817 2710 2979 6213 8412
Size: 817 1893 269 3234 2199
Fragments arranged by size:
3234 2199 1893 817 269
BlpI GC'TnA_GC
Cuts at: 0 2996 8252 8412
Size: 2996 5256 160
Fragments arranged by size:
5256 2996 160
Bme1580I G_kGCm'C
Cuts at: 0 755 6982 8412
Size: 755 6227 1430
Fragments arranged by size:
6227 1430 755
BmgBI CAC'GTC
Cuts at: 0 2446 7272 8412
Size: 2446 4826 1140
Fragments arranged by size:
4826 2446 1140
BmrI ACTGGGnnnn_n'
Cuts at: 0 511 5028 5405 6873 8412
Size: 511 4517 377 1468 1539
Fragments arranged by size:
4517 1539 1468 511 377
BmtI G_CTAG'C
Cuts at: 0 7925 8412
Size: 7925 487
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1488 4545 5901 8412
Size: 1488 3057 1356 2511
Fragments arranged by size:
3057 2511 1488 1356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 938 6694 8412
Size: 438 500 5756 1718
Fragments arranged by size:
5756 1718 500 438
Bpu10I CC'TnA_GC
Cuts at: 0 1817 5528 8412
Size: 1817 3711 2884
Fragments arranged by size:
3711 2884 1817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 855 2613 4311 6880 8412
Size: 834 21 1758 1698 2569 1532
Fragments arranged by size:
2569 1758 1698 1532 834 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 1927 2403 3884 8412
Size: 1927 476 1481 4528
Fragments arranged by size:
4528 1927 1481 476
BsaAI yAC'GTr
Cuts at: 0 6739 8412
Size: 6739 1673
BsaBI GATnn'nnATC
Cuts at: 0 1634 2159 6467 8412
Size: 1634 525 4308 1945
Fragments arranged by size:
4308 1945 1634 525
BsaHI Gr'CG_yC
Cuts at: 0 8117 8412
Size: 8117 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3408 3438 4609 4639 5266 5296 5736 5766
Size: 3408 30 1171 30 627 30 440 30
Cuts at: 5766 6269 6299 7171 7201 8221 8251 8412
Size: 503 30 872 30 1020 30 161
Fragments arranged by size:
3408 1171 1020 872 627 503 440 161
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3470 3473 4100 4416 6663 7979 8045 8048
Size: 3470 3 627 316 2247 1316 66 3
Cuts at: 8048 8412
Size: 364
Fragments arranged by size:
3470 2247 1316 627 364 316 66 3
3
BseYI C'CCAG_C
Cuts at: 0 1540 3487 6050 8412
Size: 1540 1947 2563 2362
Fragments arranged by size:
2563 2362 1947 1540
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 4835 8203 8412
Size: 1201 3634 3368 209
Fragments arranged by size:
3634 3368 1201 209
BsiHKAI G_wGCw'C
Cuts at: 0 755 1999 5942 6447 8412
Size: 755 1244 3943 505 1965
Fragments arranged by size:
3943 1965 1244 755 505
BsmI GAATG_Cn'
Cuts at: 0 801 1279 1984 8412
Size: 801 478 705 6428
Fragments arranged by size:
6428 801 705 478
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1573 8412
Size: 1573 6839
Bsp1286I G_dGCh'C
Cuts at: 0 755 1999 5942 6447 6982 8412
Size: 755 1244 3943 505 535 1430
Fragments arranged by size:
3943 1430 1244 755 535 505
BspHI T'CATG_A
Cuts at: 0 3402 8412
Size: 3402 5010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 625 2834 7002 8412
Size: 105 520 2209 4168 1410
Fragments arranged by size:
4168 2209 1410 520 105
BsrDI GCAATG_nn'
Cuts at: 0 1034 1155 2907 3755 4960 5078 5780 6077
Size: 1034 121 1752 848 1205 118 702 297
Cuts at: 6077 6141 6911 8412
Size: 64 770 1501
Fragments arranged by size:
1752 1501 1205 1034 848 770 702 297
121 118 64
BsrFI r'CCGG_y
Cuts at: 0 458 2126 8412
Size: 458 1668 6286
Fragments arranged by size:
6286 1668 458
BsrGI T'GTAC_A
Cuts at: 0 348 3980 8412
Size: 348 3632 4432
Fragments arranged by size:
4432 3632 348
BssSI C'ACGA_G
Cuts at: 0 1088 2678 8412
Size: 1088 1590 5734
Fragments arranged by size:
5734 1590 1088
BstBI TT'CG_AA
Cuts at: 0 2520 5364 8412
Size: 2520 2844 3048
Fragments arranged by size:
3048 2844 2520
BstYI r'GATC_y
Cuts at: 0 817 2539 2710 2979 4074 4894 5327 6213
Size: 817 1722 171 269 1095 820 433 886
Cuts at: 6213 6424 7128 8412
Size: 211 704 1284
Fragments arranged by size:
1722 1284 1095 886 820 817 704 433
269 211 171
Bsu36I CC'TnA_GG
Cuts at: 0 3683 6813 8412
Size: 3683 3130 1599
Fragments arranged by size:
3683 3130 1599
BtgI C'CryG_G
Cuts at: 0 7197 8412
Size: 7197 1215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 8115 8412
Size: 8115 297
BtsI GCAGTG_nn'
Cuts at: 0 258 3732 4313 7076 7954 8202 8412
Size: 258 3474 581 2763 878 248 210
Fragments arranged by size:
3474 2763 878 581 258 248 210
DraI TTT'AAA
Cuts at: 0 2730 4913 5792 6309 6483 6686 8412
Size: 2730 2183 879 517 174 203 1726
Fragments arranged by size:
2730 2183 1726 879 517 203 174
DraIII CAC_nnn'GTG
Cuts at: 0 750 3196 8412
Size: 750 2446 5216
Fragments arranged by size:
5216 2446 750
DrdI GACnn_nn'nnGTC
Cuts at: 0 277 2672 8412
Size: 277 2395 5740
Fragments arranged by size:
5740 2395 277
EaeI y'GGCC_r
Cuts at: 0 3393 4732 5139 8265 8412
Size: 3393 1339 407 3126 147
Fragments arranged by size:
3393 3126 1339 407 147
EarI CTCTTCn'nnn_
Cuts at: 0 86 137 1641 3347 3591 5690 5870 7325
Size: 86 51 1504 1706 244 2099 180 1455
Cuts at: 7325 7817 8412
Size: 492 595
Fragments arranged by size:
2099 1706 1504 1455 595 492 244 180
86 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 938 1085 1307 1721 1726 2351 2414
Size: 438 500 147 222 414 5 625 63
Cuts at: 2414 3590 3887 4219 4401 5123 6694 8027 8187
Size: 1176 297 332 182 722 1571 1333 160
Cuts at: 8187 8412
Size: 225
Fragments arranged by size:
1571 1333 1176 722 625 500 438 414
332 297 225 222 182 160 147 63
5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 208 3496 4082 5286 8412
Size: 208 3288 586 1204 3126
Fragments arranged by size:
3288 3126 1204 586 208
EcoO109I rG'GnC_Cy
Cuts at: 0 1387 6378 8412
Size: 1387 4991 2034
Fragments arranged by size:
4991 2034 1387
EcoRI G'AATT_C
Cuts at: 0 390 5991 8412
Size: 390 5601 2421
Fragments arranged by size:
5601 2421 390
EcoRV GAT'ATC
Cuts at: 0 939 1376 8412
Size: 939 437 7036
Fragments arranged by size:
7036 939 437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 262 896 2660 4723 4981 6206 8412
Size: 262 634 1764 2063 258 1225 2206
Fragments arranged by size:
2206 2063 1764 1225 634 262 258
HaeII r_GCGC'y
Cuts at: 0 1470 7169 8259 8412
Size: 1470 5699 1090 153
Fragments arranged by size:
5699 1470 1090 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 354 386 1432 1464 1669 1701
Size: 36 32 286 32 1046 32 205 32
Cuts at: 1701 1885 1917 2344 2376 2407 2439 3052 3084
Size: 184 32 427 32 31 32 613 32
Cuts at: 3084 4879 4911 5080 5112 6038 6070 6162 6194
Size: 1795 32 169 32 926 32 92 32
Cuts at: 6194 6655 6687 6796 6828 6912 6944 7144 7176
Size: 461 32 109 32 84 32 200 32
Cuts at: 7176 7426 7458 8412
Size: 250 32 954
Fragments arranged by size:
1795 1046 954 926 613 461 427 286
250 205 200 184 169 109 92 84
36 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 31
HincII GTy'rAC
Cuts at: 0 4799 5524 6367 6501 8115 8412
Size: 4799 725 843 134 1614 297
Fragments arranged by size:
4799 1614 843 725 297 134
HindIII A'AGCT_T
Cuts at: 0 3293 3338 3766 3832 5906 8412
Size: 3293 45 428 66 2074 2506
Fragments arranged by size:
3293 2506 2074 428 66 45
HpaI GTT'AAC
Cuts at: 0 6501 8412
Size: 6501 1911
MfeI C'AATT_G
Cuts at: 0 5354 8412
Size: 5354 3058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1737 4968 5773 6420 8412
Size: 1737 3231 805 647 1992
Fragments arranged by size:
3231 1992 1737 805 647
MscI TGG'CCA
Cuts at: 0 3395 4734 5141 8412
Size: 3395 1339 407 3271
Fragments arranged by size:
3395 3271 1339 407
MslI CAynn'nnrTG
Cuts at: 0 2282 2612 3401 5659 6182 6551 7060 8412
Size: 2282 330 789 2258 523 369 509 1352
Fragments arranged by size:
2282 2258 1352 789 523 509 369 330
MspA1I CmG'CkG
Cuts at: 0 75 3922 4738 8412
Size: 75 3847 816 3674
Fragments arranged by size:
3847 3674 816 75
NaeI GCC'GGC
Cuts at: 0 2128 8412
Size: 2128 6284
NdeI CA'TA_TG
Cuts at: 0 3549 7061 8412
Size: 3549 3512 1351
Fragments arranged by size:
3549 3512 1351
NgoMIV G'CCGG_C
Cuts at: 0 2126 8412
Size: 2126 6286
NheI G'CTAG_C
Cuts at: 0 7921 8412
Size: 7921 491
NsiI A_TGCA'T
Cuts at: 0 1419 5153 7352 8412
Size: 1419 3734 2199 1060
Fragments arranged by size:
3734 2199 1419 1060
NspI r_CATG'y
Cuts at: 0 336 561 3969 5819 6070 7121 8412
Size: 336 225 3408 1850 251 1051 1291
Fragments arranged by size:
3408 1850 1291 1051 336 251 225
PasI CC'CwG_GG
Cuts at: 0 7620 8412
Size: 7620 792
PflMI CCAn_nnn'nTGG
Cuts at: 0 2647 6057 8412
Size: 2647 3410 2355
Fragments arranged by size:
3410 2647 2355
PfoI T'CCnGG_A
Cuts at: 0 8239 8412
Size: 8239 173
PpuMI rG'GwC_Cy
Cuts at: 0 1387 6378 8412
Size: 1387 4991 2034
Fragments arranged by size:
4991 2034 1387
PshAI GACnn'nnGTC
Cuts at: 0 5476 8412
Size: 5476 2936
PsiI TTA'TAA
Cuts at: 0 4160 7345 8412
Size: 4160 3185 1067
Fragments arranged by size:
4160 3185 1067
PstI C_TGCA'G
Cuts at: 0 552 4320 7397 8412
Size: 552 3768 3077 1015
Fragments arranged by size:
3768 3077 1015 552
PvuII CAG'CTG
Cuts at: 0 75 4738 8412
Size: 75 4663 3674
Fragments arranged by size:
4663 3674 75
SalI G'TCGA_C
Cuts at: 0 5522 8412
Size: 5522 2890
SapI GCTCTTCn'nnn_
Cuts at: 0 3347 7325 8412
Size: 3347 3978 1087
Fragments arranged by size:
3978 3347 1087
SbfI CC_TGCA'GG
Cuts at: 0 552 8412
Size: 552 7860
ScaI AGT'ACT
Cuts at: 0 6518 7699 8412
Size: 6518 1181 713
Fragments arranged by size:
6518 1181 713
SexAI A'CCwGG_T
Cuts at: 0 209 7094 8412
Size: 209 6885 1318
Fragments arranged by size:
6885 1318 209
SfcI C'TryA_G
Cuts at: 0 548 1550 2315 3002 4288 4316 4898 5402
Size: 548 1002 765 687 1286 28 582 504
Cuts at: 5402 6248 7393 8229 8412
Size: 846 1145 836 183
Fragments arranged by size:
1286 1145 1002 846 836 765 687 582
548 504 183 28
SmlI C'TyrA_G
Cuts at: 0 813 870 920 2628 4326 6859 8412
Size: 813 57 50 1708 1698 2533 1553
Fragments arranged by size:
2533 1708 1698 1553 813 57 50
SpeI A'CTAG_T
Cuts at: 0 2740 5004 5473 8412
Size: 2740 2264 469 2939
Fragments arranged by size:
2939 2740 2264 469
SphI G_CATG'C
Cuts at: 0 336 5819 8412
Size: 336 5483 2593
Fragments arranged by size:
5483 2593 336
SspI AAT'ATT
Cuts at: 0 3624 3816 5570 6654 8412
Size: 3624 192 1754 1084 1758
Fragments arranged by size:
3624 1758 1754 1084 192
StuI AGG'CCT
Cuts at: 0 2097 2196 7497 8412
Size: 2097 99 5301 915
Fragments arranged by size:
5301 2097 915 99
StyI C'CwwG_G
Cuts at: 0 246 1055 4836 8281 8393 8412
Size: 246 809 3781 3445 112 19
Fragments arranged by size:
3781 3445 809 246 112 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1224 6790 6892 8412
Size: 1224 5566 102 1520
Fragments arranged by size:
5566 1520 1224 102
TatI w'GTAC_w
Cuts at: 0 348 3980 4013 5911 6516 6561 7697 8150
Size: 348 3632 33 1898 605 45 1136 453
Cuts at: 8150 8412
Size: 262
Fragments arranged by size:
3632 1898 1136 605 453 348 262 45
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 576 608 869 901 3408 3440
Size: 179 32 365 32 261 32 2507 32
Cuts at: 3440 7171 7203 7769 7801 8412
Size: 3731 32 566 32 611
Fragments arranged by size:
3731 2507 611 566 365 261 179 32
32 32 32 32 32
XbaI T'CTAG_A
Cuts at: 0 7854 8412
Size: 7854 558
XhoI C'TCGA_G
Cuts at: 0 920 8412
Size: 920 7492
XmnI GAAnn'nnTTC
Cuts at: 0 3417 6191 8412
Size: 3417 2774 2221
Fragments arranged by size:
3417 2774 2221
ZraI GAC'GTC
Cuts at: 0 8118 8412
Size: 8118 294
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AlfI AloI
AlwNI ApaLI ApoI AseI AvaI BaeI BanI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
NheI NsiI NspI PasI PflMI PfoI PpuMI PshAI
PsiI PstI PvuII SalI SapI SbfI ScaI SexAI
SfcI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI XbaI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AleI ApaI AscI
AsiSI AvrII BamHI BanII BbeI BsaWI BsiEI BsiWI
BspEI BsrBI BssHII BstAPI BstEII BstXI BstZ17I ClaI
CspCI EagI EciI EcoICRI FseI FspI FspAI KasI
KpnI MluI NarI NcoI NotI NruI PacI PciI
PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SanDI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI SwaI Tth111I XcmI XmaI