Restriction map for
pF1KE2345
(Linear) (Six-base) MAPSORT of: KIEE2345.seq Check: 2633 from: 1 to: 1077
KIEE2345.orf
With 182 enzymes: SgfI *
May 20, 2011 15:42 ..
Acc65I G'GTAC_C
Cuts at: 0 908 1043 1077
Size: 908 135 34
Fragments arranged by size:
908 135 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1077
Size: 972 105
AflIII A'CryG_T
Cuts at: 0 656 1077
Size: 656 421
AlwNI CAG_nnn'CTG
Cuts at: 0 72 633 921 1077
Size: 72 561 288 156
Fragments arranged by size:
561 288 156 72
ApaI G_GGCC'C
Cuts at: 0 16 823 1077
Size: 16 807 254
Fragments arranged by size:
807 254 16
ApaLI G'TGCA_C
Cuts at: 0 319 1077
Size: 319 758
AvaI C'yCGr_G
Cuts at: 0 369 1077
Size: 369 708
BamHI G'GATC_C
Cuts at: 0 3 1077
Size: 3 1074
BanI G'GyrC_C
Cuts at: 0 249 908 1043 1077
Size: 249 659 135 34
Fragments arranged by size:
659 249 135 34
BanII G_rGCy'C
Cuts at: 0 16 823 892 1077
Size: 16 807 69 185
Fragments arranged by size:
807 185 69 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 305 442 1077
Size: 305 137 635
Fragments arranged by size:
635 305 137
BglI GCCn_nnn'nGGC
Cuts at: 0 793 1077
Size: 793 284
Bme1580I G_kGCm'C
Cuts at: 0 16 323 354 809 823 999 1077
Size: 16 307 31 455 14 176 78
Fragments arranged by size:
455 307 176 78 31 16 14
BmgBI CAC'GTC
Cuts at: 0 203 657 1077
Size: 203 454 420
Fragments arranged by size:
454 420 203
BmrI ACTGGGnnnn_n'
Cuts at: 0 341 603 1077
Size: 341 262 474
Fragments arranged by size:
474 341 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 811 1077
Size: 532 279 266
Fragments arranged by size:
532 279 266
Bpu10I CC'TnA_GC
Cuts at: 0 946 1077
Size: 946 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 631 1077
Size: 95 536 446
Fragments arranged by size:
536 446 95
BsaAI yAC'GTr
Cuts at: 0 873 1077
Size: 873 204
BsaHI Gr'CG_yC
Cuts at: 0 860 1077
Size: 860 217
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 652 682 694 724 1077
Size: 505 30 117 30 12 30 353
Fragments arranged by size:
505 353 117 30 30 30 12
BseYI C'CCAG_C
Cuts at: 0 353 816 823 992 1077
Size: 353 463 7 169 85
Fragments arranged by size:
463 353 169 85 7
BsiEI CG_ry'CG
Cuts at: 0 285 1077
Size: 285 792
BsiHKAI G_wGCw'C
Cuts at: 0 323 1077
Size: 323 754
BsmBI CGTCTCn'nnnn_
Cuts at: 0 697 961 1077
Size: 697 264 116
Fragments arranged by size:
697 264 116
Bsp1286I G_dGCh'C
Cuts at: 0 16 323 354 809 823 892 999 1077
Size: 16 307 31 455 14 69 107 78
Fragments arranged by size:
455 307 107 78 69 31 16 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 920 1077
Size: 920 157
BsrBI CCG'CTC
Cuts at: 0 546 1077
Size: 546 531
BsrGI T'GTAC_A
Cuts at: 0 338 1077
Size: 338 739
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1016 1077
Size: 1016 61
BstXI CCAn_nnnn'nTGG
Cuts at: 0 900 1077
Size: 900 177
BstYI r'GATC_y
Cuts at: 0 3 392 1077
Size: 3 389 685
Fragments arranged by size:
685 389 3
BtgI C'CryG_G
Cuts at: 0 440 517 809 1077
Size: 440 77 292 268
Fragments arranged by size:
440 292 268 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 402 1077
Size: 402 675
BtsI GCAGTG_nn'
Cuts at: 0 275 420 590 1077
Size: 275 145 170 487
Fragments arranged by size:
487 275 170 145
EaeI y'GGCC_r
Cuts at: 0 17 525 557 1077
Size: 17 508 32 520
Fragments arranged by size:
520 508 32 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 597 1077
Size: 597 480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 811 972 1077
Size: 532 279 161 105
Fragments arranged by size:
532 279 161 105
EcoNI CCTnn'n_nnAGG
Cuts at: 0 685 1077
Size: 685 392
EcoO109I rG'GnC_Cy
Cuts at: 0 140 164 820 897 1077
Size: 140 24 656 77 180
Fragments arranged by size:
656 180 140 77 24
HaeII r_GCGC'y
Cuts at: 0 42 1077
Size: 42 1035
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 694 726 1077
Size: 505 32 157 32 351
Fragments arranged by size:
505 351 157 32 32
KpnI G_GTAC'C
Cuts at: 0 912 1047 1077
Size: 912 135 30
Fragments arranged by size:
912 135 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 614 1077
Size: 509 105 463
Fragments arranged by size:
509 463 105
MslI CAynn'nnrTG
Cuts at: 0 871 1077
Size: 871 206
MspA1I CmG'CkG
Cuts at: 0 69 236 432 992 1077
Size: 69 167 196 560 85
Fragments arranged by size:
560 196 167 85 69
NcoI C'CATG_G
Cuts at: 0 440 1077
Size: 440 637
PasI CC'CwG_GG
Cuts at: 0 9 687 1077
Size: 9 678 390
Fragments arranged by size:
678 390 9
PmlI CAC'GTG
Cuts at: 0 873 1077
Size: 873 204
PpuMI rG'GwC_Cy
Cuts at: 0 140 164 897 1077
Size: 140 24 733 180
Fragments arranged by size:
733 180 140 24
PspOMI G'GGCC_C
Cuts at: 0 12 819 1077
Size: 12 807 258
Fragments arranged by size:
807 258 12
PstI C_TGCA'G
Cuts at: 0 638 917 1031 1077
Size: 638 279 114 46
Fragments arranged by size:
638 279 114 46
PvuII CAG'CTG
Cuts at: 0 69 432 992 1077
Size: 69 363 560 85
Fragments arranged by size:
560 363 85 69
SanDI GG'GwC_CC
Cuts at: 0 140 164 1077
Size: 140 24 913
Fragments arranged by size:
913 140 24
SbfI CC_TGCA'GG
Cuts at: 0 638 917 1077
Size: 638 279 160
Fragments arranged by size:
638 279 160
SfcI C'TryA_G
Cuts at: 0 634 913 1027 1077
Size: 634 279 114 50
Fragments arranged by size:
634 279 114 50
SmlI C'TyrA_G
Cuts at: 0 110 646 1077
Size: 110 536 431
Fragments arranged by size:
536 431 110
StuI AGG'CCT
Cuts at: 0 796 1015 1077
Size: 796 219 62
Fragments arranged by size:
796 219 62
StyI C'CwwG_G
Cuts at: 0 440 893 1077
Size: 440 453 184
Fragments arranged by size:
453 440 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 554 580 1077
Size: 554 26 497
Fragments arranged by size:
554 497 26
TatI w'GTAC_w
Cuts at: 0 338 1077
Size: 338 739
Tth111I GACn'n_nGTC
Cuts at: 0 310 708 1077
Size: 310 398 369
Fragments arranged by size:
398 369 310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 32 422 567 1077
Size: 32 390 145 510
Fragments arranged by size:
510 390 145 32
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BciVI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrGI BstAPI BstXI BstYI
BtgI BtgZI BtsI EaeI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I KpnI MmeI MslI MspA1I NcoI PasI
PmlI PpuMI PspOMI PstI PvuII SanDI SbfI SfcI
SmlI StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BcgI BclI BfrBI BglII
BlpI BmtI BplI BsaI BsaBI BsaWI BseRI BsgI
BsiWI BsmI BspEI BspHI BsrDI BsrFI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XhoI XmaI XmnI ZraI