Restriction map for
pF1KE2334
(Linear) (Six-base) MAPSORT of: KIEE2334.seq Check: 5601 from: 1 to: 4665
KIEE2334.orf
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AccI GT'mk_AC
Cuts at: 0 1352 4665
Size: 1352 3313
Acc65I G'GTAC_C
Cuts at: 0 277 4665
Size: 277 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1634 2671 2677 3128 3392 3536 3856 4285
Size: 1634 1037 6 451 264 144 320 429
Cuts at: 4285 4665
Size: 380
Fragments arranged by size:
1634 1037 451 429 380 320 264 144
6
AflIII A'CryG_T
Cuts at: 0 1761 4665
Size: 1761 2904
AleI CACnn'nnGTG
Cuts at: 0 811 4665
Size: 811 3854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3838 4665
Size: 3838 827
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1634 1666 3058 3090 4665
Size: 1634 32 1392 32 1575
Fragments arranged by size:
1634 1575 1392 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 327 514 1710 2331 2536 4190 4665
Size: 327 187 1196 621 205 1654 475
Fragments arranged by size:
1654 1196 621 475 327 205 187
ApaLI G'TGCA_C
Cuts at: 0 4244 4665
Size: 4244 421
ApoI r'AATT_y
Cuts at: 0 223 231 345 355 616 1053 3178 3216
Size: 223 8 114 10 261 437 2125 38
Cuts at: 3216 3530 3859 4665
Size: 314 329 806
Fragments arranged by size:
2125 806 437 329 314 261 223 114
38 10 8
AseI AT'TA_AT
Cuts at: 0 3767 4665
Size: 3767 898
AvaI C'yCGr_G
Cuts at: 0 1253 4665
Size: 1253 3412
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 770 803 4665
Size: 770 33 3862
Fragments arranged by size:
3862 770 33
BamHI G'GATC_C
Cuts at: 0 3488 4665
Size: 3488 1177
BanI G'GyrC_C
Cuts at: 0 277 945 4386 4665
Size: 277 668 3441 279
Fragments arranged by size:
3441 668 279 277
BanII G_rGCy'C
Cuts at: 0 1099 2723 4665
Size: 1099 1624 1942
Fragments arranged by size:
1942 1624 1099
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 475 2591 2915 3659 3736 4624 4665
Size: 215 260 2116 324 744 77 888 41
Fragments arranged by size:
2116 888 744 324 260 215 77 41
BclI T'GATC_A
Cuts at: 0 2031 2384 2678 4077 4353 4665
Size: 2031 353 294 1399 276 312
Fragments arranged by size:
2031 1399 353 312 294 276
BfrBI ATG'CAT
Cuts at: 0 1968 4665
Size: 1968 2697
BglI GCCn_nnn'nGGC
Cuts at: 0 3545 3969 4665
Size: 3545 424 696
Fragments arranged by size:
3545 696 424
BglII A'GATC_T
Cuts at: 0 873 972 2426 4095 4359 4665
Size: 873 99 1454 1669 264 306
Fragments arranged by size:
1669 1454 873 306 264 99
BlpI GC'TnA_GC
Cuts at: 0 3032 4665
Size: 3032 1633
Bme1580I G_kGCm'C
Cuts at: 0 4248 4665
Size: 4248 417
BmrI ACTGGGnnnn_n'
Cuts at: 0 4215 4665
Size: 4215 450
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 3026 4665
Size: 624 2402 1639
Fragments arranged by size:
2402 1639 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 910 1184 1411 1661 1760 1798 1898
Size: 322 588 274 227 250 99 38 100
Cuts at: 1898 1999 2415 2728 4665
Size: 101 416 313 1937
Fragments arranged by size:
1937 588 416 322 313 274 250 227
101 100 99 38
Bpu10I CC'TnA_GC
Cuts at: 0 3161 4665
Size: 3161 1504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 4391 4665
Size: 511 3880 274
Fragments arranged by size:
3880 511 274
BsaI GGTCTCn'nnnn_
Cuts at: 0 1516 1975 4665
Size: 1516 459 2690
Fragments arranged by size:
2690 1516 459
BsaBI GATnn'nnATC
Cuts at: 0 1691 1841 4076 4358 4665
Size: 1691 150 2235 282 307
Fragments arranged by size:
2235 1691 307 282 150
BsaHI Gr'CG_yC
Cuts at: 0 4442 4665
Size: 4442 223
BsaWI w'CCGG_w
Cuts at: 0 1358 4665
Size: 1358 3307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 68 98 883 913 1634 1664 1733 1763
Size: 68 30 785 30 721 30 69 30
Cuts at: 1763 3540 3570 3834 3864 4665
Size: 1777 30 264 30 801
Fragments arranged by size:
1777 801 785 721 264 69 68 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 102 2737 4665
Size: 102 2635 1928
Fragments arranged by size:
2635 1928 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1499 4665
Size: 862 637 3166
Fragments arranged by size:
3166 862 637
BsiHKAI G_wGCw'C
Cuts at: 0 757 934 1099 2723 4134 4248 4605 4665
Size: 757 177 165 1624 1411 114 357 60
Fragments arranged by size:
1624 1411 757 357 177 165 114 60
BsmI GAATG_Cn'
Cuts at: 0 1919 4665
Size: 1919 2746
BsmBI CGTCTCn'nnnn_
Cuts at: 0 8 4665
Size: 8 4657
Bsp1286I G_dGCh'C
Cuts at: 0 757 934 1099 2723 4134 4248 4605 4665
Size: 757 177 165 1624 1411 114 357 60
Fragments arranged by size:
1624 1411 757 357 177 165 114 60
BspEI T'CCGG_A
Cuts at: 0 1358 4665
Size: 1358 3307
BspHI T'CATG_A
Cuts at: 0 270 1034 2054 4665
Size: 270 764 1020 2611
Fragments arranged by size:
2611 1020 764 270
BsrBI CCG'CTC
Cuts at: 0 23 2005 4665
Size: 23 1982 2660
Fragments arranged by size:
2660 1982 23
BsrDI GCAATG_nn'
Cuts at: 0 660 2540 2691 3348 4665
Size: 660 1880 151 657 1317
Fragments arranged by size:
1880 1317 660 657 151
BsrFI r'CCGG_y
Cuts at: 0 48 4665
Size: 48 4617
BsrGI T'GTAC_A
Cuts at: 0 4607 4665
Size: 4607 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1345 4665
Size: 1345 3320
BstXI CCAn_nnnn'nTGG
Cuts at: 0 942 3441 3485 4665
Size: 942 2499 44 1180
Fragments arranged by size:
2499 1180 942 44
BstYI r'GATC_y
Cuts at: 0 873 972 1041 2426 2880 3488 4016 4095
Size: 873 99 69 1385 454 608 528 79
Cuts at: 4095 4260 4359 4571 4665
Size: 165 99 212 94
Fragments arranged by size:
1385 873 608 528 454 212 165 99
99 94 79 69
Bsu36I CC'TnA_GG
Cuts at: 0 2772 3399 4665
Size: 2772 627 1266
Fragments arranged by size:
2772 1266 627
BtgI C'CryG_G
Cuts at: 0 35 3379 4665
Size: 35 3344 1286
Fragments arranged by size:
3344 1286 35
BtsI GCAGTG_nn'
Cuts at: 0 2681 4665
Size: 2681 1984
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 798 833 4665
Size: 798 35 3832
Fragments arranged by size:
3832 798 35
DraI TTT'AAA
Cuts at: 0 354 953 2583 3098 4665
Size: 354 599 1630 515 1567
Fragments arranged by size:
1630 1567 599 515 354
EaeI y'GGCC_r
Cuts at: 0 743 2498 3443 4665
Size: 743 1755 945 1222
Fragments arranged by size:
1755 1222 945 743
EarI CTCTTCn'nnn_
Cuts at: 0 503 1115 1183 2861 3284 4027 4161 4665
Size: 503 612 68 1678 423 743 134 504
Fragments arranged by size:
1678 743 612 504 503 423 134 68
EcoICRI GAG'CTC
Cuts at: 0 1097 2721 4665
Size: 1097 1624 1944
Fragments arranged by size:
1944 1624 1097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 910 1184 1411 1634 1661 1760 1798
Size: 322 588 274 227 223 27 99 38
Cuts at: 1798 1898 1999 2415 2671 2677 2728 3128 3392
Size: 100 101 416 256 6 51 400 264
Cuts at: 3392 3536 3856 4285 4665
Size: 144 320 429 380
Fragments arranged by size:
588 429 416 400 380 322 320 274
264 256 227 223 144 101 100 99
51 38 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1923 4665
Size: 1923 2742
EcoO109I rG'GnC_Cy
Cuts at: 0 2520 4329 4642 4665
Size: 2520 1809 313 23
Fragments arranged by size:
2520 1809 313 23
EcoRV GAT'ATC
Cuts at: 0 556 4665
Size: 556 4109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1456 3897 4038 4432 4665
Size: 1456 2441 141 394 233
Fragments arranged by size:
2441 1456 394 233 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 978 1010 2156 2188 2376 2408 3473 3505
Size: 978 32 1146 32 188 32 1065 32
Cuts at: 3505 3670 3702 4429 4461 4665
Size: 165 32 727 32 204
Fragments arranged by size:
1146 1065 978 727 204 188 165 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1383 2979 4053 4665
Size: 1383 1596 1074 612
Fragments arranged by size:
1596 1383 1074 612
HindIII A'AGCT_T
Cuts at: 0 3102 4665
Size: 3102 1563
KpnI G_GTAC'C
Cuts at: 0 281 4665
Size: 281 4384
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1863 2492 4665
Size: 1863 629 2173
Fragments arranged by size:
2173 1863 629
MscI TGG'CCA
Cuts at: 0 745 2500 3445 4665
Size: 745 1755 945 1220
Fragments arranged by size:
1755 1220 945 745
MslI CAynn'nnrTG
Cuts at: 0 811 1963 2494 3439 3820 4241 4665
Size: 811 1152 531 945 381 421 424
Fragments arranged by size:
1152 945 811 531 424 421 381
MspA1I CmG'CkG
Cuts at: 0 37 415 1090 1549 2104 3036 3465 4516
Size: 37 378 675 459 555 932 429 1051
Cuts at: 4516 4665
Size: 149
Fragments arranged by size:
1051 932 675 555 459 429 378 149
37
NdeI CA'TA_TG
Cuts at: 0 3264 4665
Size: 3264 1401
NsiI A_TGCA'T
Cuts at: 0 1970 4665
Size: 1970 2695
NspI r_CATG'y
Cuts at: 0 1765 3093 4665
Size: 1765 1328 1572
Fragments arranged by size:
1765 1572 1328
PciI A'CATG_T
Cuts at: 0 1761 4665
Size: 1761 2904
PflMI CCAn_nnn'nTGG
Cuts at: 0 1683 4665
Size: 1683 2982
PfoI T'CCnGG_A
Cuts at: 0 1775 1976 4025 4529 4665
Size: 1775 201 2049 504 136
Fragments arranged by size:
2049 1775 504 201 136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1634 1666 4665
Size: 1634 32 2999
Fragments arranged by size:
2999 1634 32
PpuMI rG'GwC_Cy
Cuts at: 0 4329 4642 4665
Size: 4329 313 23
Fragments arranged by size:
4329 313 23
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 80 112 4665
Size: 80 32 4553
Fragments arranged by size:
4553 80 32
PstI C_TGCA'G
Cuts at: 0 2517 2532 2641 4665
Size: 2517 15 109 2024
Fragments arranged by size:
2517 2024 109 15
PvuII CAG'CTG
Cuts at: 0 415 1090 1549 2104 3036 3465 4516 4665
Size: 415 675 459 555 932 429 1051 149
Fragments arranged by size:
1051 932 675 555 459 429 415 149
SacI G_AGCT'C
Cuts at: 0 1099 2723 4665
Size: 1099 1624 1942
Fragments arranged by size:
1942 1624 1099
SacII CC_GC'GG
Cuts at: 0 38 4665
Size: 38 4627
SapI GCTCTTCn'nnn_
Cuts at: 0 503 2861 4665
Size: 503 2358 1804
Fragments arranged by size:
2358 1804 503
SfcI C'TryA_G
Cuts at: 0 2201 2513 2528 2637 3388 4182 4567 4665
Size: 2201 312 15 109 751 794 385 98
Fragments arranged by size:
2201 794 751 385 312 109 98 15
SmlI C'TyrA_G
Cuts at: 0 490 4406 4665
Size: 490 3916 259
Fragments arranged by size:
3916 490 259
SpeI A'CTAG_T
Cuts at: 0 4044 4665
Size: 4044 621
SspI AAT'ATT
Cuts at: 0 255 305 3279 3771 4665
Size: 255 50 2974 492 894
Fragments arranged by size:
2974 894 492 255 50
StuI AGG'CCT
Cuts at: 0 1292 4665
Size: 1292 3373
StyI C'CwwG_G
Cuts at: 0 770 2184 2246 2501 4314 4665
Size: 770 1414 62 255 1813 351
Fragments arranged by size:
1813 1414 770 351 255 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2005 4665
Size: 2005 2660
TatI w'GTAC_w
Cuts at: 0 87 761 2009 2633 3953 4032 4607 4665
Size: 87 674 1248 624 1320 79 575 58
Fragments arranged by size:
1320 1248 674 624 575 87 79 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1733 1765 3540 3572 4665
Size: 1733 32 1775 32 1093
Fragments arranged by size:
1775 1733 1093 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2983 4665
Size: 2983 1682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2285 4665
Size: 2285 2380
XmnI GAAnn'nnTTC
Cuts at: 0 1135 2085 3806 4665
Size: 1135 950 1721 859
Fragments arranged by size:
1721 1135 950 859
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApaLI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtsI CspCI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII HindIII
KpnI MmeI MscI MslI MspA1I NdeI NsiI NspI
PciI PflMI PfoI PpiI PpuMI PsrI PstI PvuII
SacI SacII SapI SfcI SmlI SpeI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI ApaI
AscI AsiSI AvrII BbeI BbvCI BcgI BciVI BmgBI
BmtI BsaAI BseYI BsiEI BsiWI BspMI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI DraIII DrdI EagI
EciI EcoRI FseI FspI FspAI HaeII HpaI KasI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PshAI PsiI PspOMI
PspXI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SwaI XbaI XhoI XmaI ZraI