Restriction map for
pF1KE2328
(Linear) (Six-base) MAPSORT of: KIEE2328.seq Check: 1938 from: 1 to: 1065
KIEE2328.orf
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AccI GT'mk_AC
Cuts at: 0 134 1065
Size: 134 931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1065
Size: 925 140
AfeI AGC'GCT
Cuts at: 0 70 1065
Size: 70 995
AflIII A'CryG_T
Cuts at: 0 581 1065
Size: 581 484
AhdI GACnn_n'nnGTC
Cuts at: 0 24 1065
Size: 24 1041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 577 1065
Size: 577 488
AlwNI CAG_nnn'CTG
Cuts at: 0 119 627 717 1065
Size: 119 508 90 348
Fragments arranged by size:
508 348 119 90
ApaLI G'TGCA_C
Cuts at: 0 993 1065
Size: 993 72
AseI AT'TA_AT
Cuts at: 0 847 1065
Size: 847 218
AvaI C'yCGr_G
Cuts at: 0 10 31 1065
Size: 10 21 1034
Fragments arranged by size:
1034 21 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 479 788 1065
Size: 55 424 309 277
Fragments arranged by size:
424 309 277 55
BbvCI CC'TCA_GC
Cuts at: 0 109 1065
Size: 109 956
BlpI GC'TnA_GC
Cuts at: 0 718 775 1065
Size: 718 57 290
Fragments arranged by size:
718 290 57
Bme1580I G_kGCm'C
Cuts at: 0 982 997 1065
Size: 982 15 68
Fragments arranged by size:
982 68 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 1065
Size: 51 1014
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1035 1065
Size: 1035 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1065
Size: 493 572
Bpu10I CC'TnA_GC
Cuts at: 0 109 1065
Size: 109 956
BsaAI yAC'GTr
Cuts at: 0 363 771 1065
Size: 363 408 294
Fragments arranged by size:
408 363 294
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 805 835 1065
Size: 805 30 230
Fragments arranged by size:
805 230 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1065
Size: 751 314
BsiEI CG_ry'CG
Cuts at: 0 56 1065
Size: 56 1009
BsiHKAI G_wGCw'C
Cuts at: 0 776 997 1065
Size: 776 221 68
Fragments arranged by size:
776 221 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 595 1065
Size: 595 470
Bsp1286I G_dGCh'C
Cuts at: 0 776 982 997 1065
Size: 776 206 15 68
Fragments arranged by size:
776 206 68 15
BspHI T'CATG_A
Cuts at: 0 434 1065
Size: 434 631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 689 773 1065
Size: 689 84 292
Fragments arranged by size:
689 292 84
BsrBI CCG'CTC
Cuts at: 0 89 828 1065
Size: 89 739 237
Fragments arranged by size:
739 237 89
BsrFI r'CCGG_y
Cuts at: 0 785 1065
Size: 785 280
BsrGI T'GTAC_A
Cuts at: 0 532 1065
Size: 532 533
BstAPI GCAn_nnn'nTGC
Cuts at: 0 119 1065
Size: 119 946
BstYI r'GATC_y
Cuts at: 0 248 833 1065
Size: 248 585 232
Fragments arranged by size:
585 248 232
BstZ17I GTA'TAC
Cuts at: 0 135 1065
Size: 135 930
BtgI C'CryG_G
Cuts at: 0 282 1065
Size: 282 783
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 402 1065
Size: 402 663
BtsI GCAGTG_nn'
Cuts at: 0 995 1065
Size: 995 70
EaeI y'GGCC_r
Cuts at: 0 53 1065
Size: 53 1012
EagI C'GGCC_G
Cuts at: 0 53 1065
Size: 53 1012
EarI CTCTTCn'nnn_
Cuts at: 0 79 181 1065
Size: 79 102 884
Fragments arranged by size:
884 102 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 925 1065
Size: 493 432 140
Fragments arranged by size:
493 432 140
EcoNI CCTnn'n_nnAGG
Cuts at: 0 13 748 1065
Size: 13 735 317
Fragments arranged by size:
735 317 13
EcoO109I rG'GnC_Cy
Cuts at: 0 732 1065
Size: 732 333
HaeII r_GCGC'y
Cuts at: 0 72 282 1065
Size: 72 210 783
Fragments arranged by size:
783 210 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 1065
Size: 10 32 1023
Fragments arranged by size:
1023 32 10
HindIII A'AGCT_T
Cuts at: 0 305 1065
Size: 305 760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 1065
Size: 356 709
MslI CAynn'nnrTG
Cuts at: 0 528 580 586 1065
Size: 528 52 6 479
Fragments arranged by size:
528 479 52 6
MspA1I CmG'CkG
Cuts at: 0 627 870 1065
Size: 627 243 195
Fragments arranged by size:
627 243 195
NcoI C'CATG_G
Cuts at: 0 282 1065
Size: 282 783
NspI r_CATG'y
Cuts at: 0 204 585 1065
Size: 204 381 480
Fragments arranged by size:
480 381 204
PasI CC'CwG_GG
Cuts at: 0 297 1065
Size: 297 768
PciI A'CATG_T
Cuts at: 0 581 1065
Size: 581 484
PfoI T'CCnGG_A
Cuts at: 0 74 970 1065
Size: 74 896 95
Fragments arranged by size:
896 95 74
PmlI CAC'GTG
Cuts at: 0 771 1065
Size: 771 294
PpuMI rG'GwC_Cy
Cuts at: 0 732 1065
Size: 732 333
PvuII CAG'CTG
Cuts at: 0 627 870 1065
Size: 627 243 195
Fragments arranged by size:
627 243 195
SapI GCTCTTCn'nnn_
Cuts at: 0 79 1065
Size: 79 986
ScaI AGT'ACT
Cuts at: 0 270 1065
Size: 270 795
SexAI A'CCwGG_T
Cuts at: 0 734 1065
Size: 734 331
SmaI CCC'GGG
Cuts at: 0 33 1065
Size: 33 1032
SspI AAT'ATT
Cuts at: 0 441 1065
Size: 441 624
StuI AGG'CCT
Cuts at: 0 651 665 1065
Size: 651 14 400
Fragments arranged by size:
651 400 14
StyI C'CwwG_G
Cuts at: 0 282 913 1065
Size: 282 631 152
Fragments arranged by size:
631 282 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 576 1065
Size: 33 543 489
Fragments arranged by size:
543 489 33
TatI w'GTAC_w
Cuts at: 0 268 532 1065
Size: 268 264 533
Fragments arranged by size:
533 268 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 952 972 984 1004 1065
Size: 952 20 12 20 61
Fragments arranged by size:
952 61 20 20 12
Tth111I GACn'n_nGTC
Cuts at: 0 736 1065
Size: 736 329
XmaI C'CCGG_G
Cuts at: 0 31 1065
Size: 31 1034
XmnI GAAnn'nnTTC
Cuts at: 0 70 151 1065
Size: 70 81 914
Fragments arranged by size:
914 81 70
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AlfI AlwNI ApaLI
AseI AvaI BbsI BbvCI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaAI BsaXI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstYI
BstZ17I BtgI BtgZI BtsI EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII MmeI MslI MspA1I
NcoI NspI PasI PciI PfoI PmlI PpuMI PvuII
SapI ScaI SexAI SmaI SspI StuI StyI TaqII
TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AleI AloI
ApaI ApoI AscI AsiSI AvrII BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmtI BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsiWI BsmI BspEI BsrDI BssHII BssSI BstBI
BstEII BstXI Bsu36I ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SwaI XbaI XcmI XhoI ZraI