Restriction map for
pF1KE2320
(Linear) (Six-base) MAPSORT of: KIEE2320.seq Check: 1972 from: 1 to: 1758
KIEE2320.orf
With 182 enzymes: SgfI *
January 11, 2011 13:49 ..
AccI GT'mk_AC
Cuts at: 0 407 1758
Size: 407 1351
Acc65I G'GTAC_C
Cuts at: 0 579 1758
Size: 579 1179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1042 1758
Size: 331 711 716
Fragments arranged by size:
716 711 331
AflIII A'CryG_T
Cuts at: 0 251 1758
Size: 251 1507
AleI CACnn'nnGTG
Cuts at: 0 433 1758
Size: 433 1325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 1261 1758
Size: 357 904 497
Fragments arranged by size:
904 497 357
AlwNI CAG_nnn'CTG
Cuts at: 0 1425 1758
Size: 1425 333
ApoI r'AATT_y
Cuts at: 0 472 610 1543 1758
Size: 472 138 933 215
Fragments arranged by size:
933 472 215 138
AvaI C'yCGr_G
Cuts at: 0 1616 1679 1758
Size: 1616 63 79
Fragments arranged by size:
1616 79 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1327 1360 1758
Size: 1327 33 398
Fragments arranged by size:
1327 398 33
BanI G'GyrC_C
Cuts at: 0 150 446 579 1237 1758
Size: 150 296 133 658 521
Fragments arranged by size:
658 521 296 150 133
BanII G_rGCy'C
Cuts at: 0 65 1606 1758
Size: 65 1541 152
Fragments arranged by size:
1541 152 65
BbeI G_GCGC'C
Cuts at: 0 154 1758
Size: 154 1604
BbsI GAAGACnn'nnnn_
Cuts at: 0 570 1758
Size: 570 1188
BbvCI CC'TCA_GC
Cuts at: 0 1001 1169 1758
Size: 1001 168 589
Fragments arranged by size:
1001 589 168
BciVI GTATCCnnnnn_n'
Cuts at: 0 880 1355 1758
Size: 880 475 403
Fragments arranged by size:
880 475 403
BglI GCCn_nnn'nGGC
Cuts at: 0 283 1758
Size: 283 1475
BlpI GC'TnA_GC
Cuts at: 0 419 463 1758
Size: 419 44 1295
Fragments arranged by size:
1295 419 44
Bme1580I G_kGCm'C
Cuts at: 0 451 1240 1758
Size: 451 789 518
Fragments arranged by size:
789 518 451
BmgBI CAC'GTC
Cuts at: 0 252 426 1758
Size: 252 174 1332
Fragments arranged by size:
1332 252 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1758
Size: 531 1227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1470 1758
Size: 1470 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1277 1590 1758
Size: 1277 313 168
Fragments arranged by size:
1277 313 168
Bpu10I CC'TnA_GC
Cuts at: 0 1001 1169 1487 1758
Size: 1001 168 318 271
Fragments arranged by size:
1001 318 271 168
BsaI GGTCTCn'nnnn_
Cuts at: 0 1606 1758
Size: 1606 152
BsaHI Gr'CG_yC
Cuts at: 0 151 1758
Size: 151 1607
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 357 387 1531 1561 1758
Size: 357 30 1144 30 197
Fragments arranged by size:
1144 357 197 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 365 1539 1758
Size: 90 275 1174 219
Fragments arranged by size:
1174 275 219 90
BseYI C'CCAG_C
Cuts at: 0 172 1758
Size: 172 1586
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1311 1758
Size: 1311 447
BsiHKAI G_wGCw'C
Cuts at: 0 274 425 1758
Size: 274 151 1333
Fragments arranged by size:
1333 274 151
Bsp1286I G_dGCh'C
Cuts at: 0 65 274 425 451 1240 1606 1758
Size: 65 209 151 26 789 366 152
Fragments arranged by size:
789 366 209 152 151 65 26
BsrFI r'CCGG_y
Cuts at: 0 1529 1758
Size: 1529 229
BsrGI T'GTAC_A
Cuts at: 0 626 1758
Size: 626 1132
BstYI r'GATC_y
Cuts at: 0 549 746 898 1758
Size: 549 197 152 860
Fragments arranged by size:
860 549 197 152
Bsu36I CC'TnA_GG
Cuts at: 0 377 1758
Size: 377 1381
BtgI C'CryG_G
Cuts at: 0 1457 1758
Size: 1457 301
BtsI GCAGTG_nn'
Cuts at: 0 1323 1587 1758
Size: 1323 264 171
Fragments arranged by size:
1323 264 171
EaeI y'GGCC_r
Cuts at: 0 284 1758
Size: 284 1474
EarI CTCTTCn'nnn_
Cuts at: 0 759 1758
Size: 759 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 793 1645 1758
Size: 793 852 113
Fragments arranged by size:
852 793 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1042 1277 1590 1758
Size: 331 711 235 313 168
Fragments arranged by size:
711 331 313 235 168
EcoRI G'AATT_C
Cuts at: 0 472 610 1543 1758
Size: 472 138 933 215
Fragments arranged by size:
933 472 215 138
HaeII r_GCGC'y
Cuts at: 0 154 846 1758
Size: 154 692 912
Fragments arranged by size:
912 692 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 357 374 389 406 480 512
Size: 178 32 147 17 15 17 74 32
Cuts at: 512 1490 1522 1637 1669 1758
Size: 978 32 115 32 89
Fragments arranged by size:
978 178 147 115 89 74 32 32
32 32 17 17 15
KasI G'GCGC_C
Cuts at: 0 150 1758
Size: 150 1608
KpnI G_GTAC'C
Cuts at: 0 583 1758
Size: 583 1175
MfeI C'AATT_G
Cuts at: 0 1010 1758
Size: 1010 748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 636 1758
Size: 242 394 1122
Fragments arranged by size:
1122 394 242
MslI CAynn'nnrTG
Cuts at: 0 433 1228 1243 1249 1758
Size: 433 795 15 6 509
Fragments arranged by size:
795 509 433 15 6
NarI GG'CG_CC
Cuts at: 0 151 1758
Size: 151 1607
NcoI C'CATG_G
Cuts at: 0 1457 1758
Size: 1457 301
NdeI CA'TA_TG
Cuts at: 0 1092 1758
Size: 1092 666
NspI r_CATG'y
Cuts at: 0 344 1248 1504 1758
Size: 344 904 256 254
Fragments arranged by size:
904 344 256 254
PasI CC'CwG_GG
Cuts at: 0 1419 1758
Size: 1419 339
PflMI CCAn_nnn'nTGG
Cuts at: 0 1255 1758
Size: 1255 503
PfoI T'CCnGG_A
Cuts at: 0 1520 1758
Size: 1520 238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1758
Size: 85 32 1641
Fragments arranged by size:
1641 85 32
SfcI C'TryA_G
Cuts at: 0 1589 1758
Size: 1589 169
SfoI GGC'GCC
Cuts at: 0 152 1758
Size: 152 1606
SmaI CCC'GGG
Cuts at: 0 1618 1758
Size: 1618 140
StyI C'CwwG_G
Cuts at: 0 1457 1758
Size: 1457 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1255 1297 1758
Size: 1255 42 461
Fragments arranged by size:
1255 461 42
TatI w'GTAC_w
Cuts at: 0 47 626 817 1758
Size: 47 579 191 941
Fragments arranged by size:
941 579 191 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 194 226 854 886 1531 1563 1758
Size: 194 32 628 32 645 32 195
Fragments arranged by size:
645 628 195 194 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1464 1758
Size: 1464 294
XmaI C'CCGG_G
Cuts at: 0 1616 1758
Size: 1616 142
XmnI GAAnn'nnTTC
Cuts at: 0 1696 1758
Size: 1696 62
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI AlwNI ApoI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BciVI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrFI BsrGI BstYI Bsu36I BtgI BtsI EaeI EarI
EciI Eco57MI EcoRI HaeII Hin4I KasI KpnI MfeI
MmeI MslI NarI NcoI NdeI NspI PasI PflMI
PfoI PpiI SfcI SfoI SmaI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BcgI
BclI BfrBI BglII BmtI BpuEI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MluI MscI MspA1I NaeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI ZraI