Restriction map for
pF1KE2310
(Linear) (Six-base) MAPSORT of: KIEE2310.seq Check: 8941 from: 1 to: 3924
KIEE2310.orf
With 182 enzymes: SgfI *
January 5, 2011 13:33 ..
AccI GT'mk_AC
Cuts at: 0 446 800 2673 2889 3924
Size: 446 354 1873 216 1035
Fragments arranged by size:
1873 1035 446 354 216
Acc65I G'GTAC_C
Cuts at: 0 2211 3924
Size: 2211 1713
AclI AA'CG_TT
Cuts at: 0 2811 3924
Size: 2811 1113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1224 1369 1416 1514 1560 2173 2237 3685
Size: 1224 145 47 98 46 613 64 1448
Cuts at: 3685 3742 3889 3924
Size: 57 147 35
Fragments arranged by size:
1448 1224 613 147 145 98 64 57
47 46 35
AflIII A'CryG_T
Cuts at: 0 257 3924
Size: 257 3667
AhdI GACnn_n'nnGTC
Cuts at: 0 22 673 2270 3924
Size: 22 651 1597 1654
Fragments arranged by size:
1654 1597 651 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3901 3924
Size: 3901 23
AlwNI CAG_nnn'CTG
Cuts at: 0 683 1347 3880 3924
Size: 683 664 2533 44
Fragments arranged by size:
2533 683 664 44
ApoI r'AATT_y
Cuts at: 0 300 457 750 1063 1092 1624 2686 2768
Size: 300 157 293 313 29 532 1062 82
Cuts at: 2768 2896 3924
Size: 128 1028
Fragments arranged by size:
1062 1028 532 313 300 293 157 128
82 29
AvaI C'yCGr_G
Cuts at: 0 2933 3924
Size: 2933 991
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 118 151 3924
Size: 118 33 3773
Fragments arranged by size:
3773 118 33
BamHI G'GATC_C
Cuts at: 0 3818 3924
Size: 3818 106
BanI G'GyrC_C
Cuts at: 0 2211 3325 3345 3924
Size: 2211 1114 20 579
Fragments arranged by size:
2211 1114 579 20
BanII G_rGCy'C
Cuts at: 0 112 1982 2368 3249 3421 3924
Size: 112 1870 386 881 172 503
Fragments arranged by size:
1870 881 503 386 172 112
BbvCI CC'TCA_GC
Cuts at: 0 2819 3056 3924
Size: 2819 237 868
Fragments arranged by size:
2819 868 237
BclI T'GATC_A
Cuts at: 0 2309 3924
Size: 2309 1615
BfrBI ATG'CAT
Cuts at: 0 3571 3924
Size: 3571 353
Bme1580I G_kGCm'C
Cuts at: 0 3239 3924
Size: 3239 685
BmrI ACTGGGnnnn_n'
Cuts at: 0 1665 2093 2981 3924
Size: 1665 428 888 943
Fragments arranged by size:
1665 943 888 428
BmtI G_CTAG'C
Cuts at: 0 2593 3924
Size: 2593 1331
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1662 3924
Size: 1662 2262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 316 725 844 1985 2686 3924
Size: 208 108 409 119 1141 701 1238
Fragments arranged by size:
1238 1141 701 409 208 119 108
Bpu10I CC'TnA_GC
Cuts at: 0 714 2175 2483 2819 3056 3924
Size: 714 1461 308 336 237 868
Fragments arranged by size:
1461 868 714 336 308 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1294 2076 3924
Size: 1294 782 1848
Fragments arranged by size:
1848 1294 782
BsaI GGTCTCn'nnnn_
Cuts at: 0 2930 3527 3924
Size: 2930 597 397
Fragments arranged by size:
2930 597 397
BsaAI yAC'GTr
Cuts at: 0 258 3924
Size: 258 3666
BsaBI GATnn'nnATC
Cuts at: 0 1829 3924
Size: 1829 2095
BsaWI w'CCGG_w
Cuts at: 0 2342 3908 3924
Size: 2342 1566 16
Fragments arranged by size:
2342 1566 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 428 458 758 788 2213 2243 3218 3248
Size: 428 30 300 30 1425 30 975 30
Cuts at: 3248 3924
Size: 676
Fragments arranged by size:
1425 975 676 428 300 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1687 3073 3146 3440 3514 3924
Size: 1687 1386 73 294 74 410
Fragments arranged by size:
1687 1386 410 294 74 73
BseYI C'CCAG_C
Cuts at: 0 64 590 2349 3924
Size: 64 526 1759 1575
Fragments arranged by size:
1759 1575 526 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 659 1845 2817 3924
Size: 107 552 1186 972 1107
Fragments arranged by size:
1186 1107 972 552 107
BsiHKAI G_wGCw'C
Cuts at: 0 112 209 830 1741 2432 3754 3924
Size: 112 97 621 911 691 1322 170
Fragments arranged by size:
1322 911 691 621 170 112 97
BsmI GAATG_Cn'
Cuts at: 0 859 3924
Size: 859 3065
Bsp1286I G_dGCh'C
Cuts at: 0 112 209 830 1741 1982 2368 2432 3239
Size: 112 97 621 911 241 386 64 807
Cuts at: 3239 3249 3421 3754 3924
Size: 10 172 333 170
Fragments arranged by size:
911 807 621 386 333 241 172 170
112 97 64 10
BspHI T'CATG_A
Cuts at: 0 1890 3924
Size: 1890 2034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 296 3782 3924
Size: 296 3486 142
Fragments arranged by size:
3486 296 142
BsrDI GCAATG_nn'
Cuts at: 0 609 3924
Size: 609 3315
BsrFI r'CCGG_y
Cuts at: 0 6 3924
Size: 6 3918
BsrGI T'GTAC_A
Cuts at: 0 1729 3924
Size: 1729 2195
BssSI C'ACGA_G
Cuts at: 0 40 2425 3422 3924
Size: 40 2385 997 502
Fragments arranged by size:
2385 997 502 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3286 3924
Size: 3286 638
BstEII G'GTnAC_C
Cuts at: 0 1260 3924
Size: 1260 2664
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1656 2379 2601 2654 3645 3924
Size: 1656 723 222 53 991 279
Fragments arranged by size:
1656 991 723 279 222 53
BstYI r'GATC_y
Cuts at: 0 1589 3818 3924
Size: 1589 2229 106
Fragments arranged by size:
2229 1589 106
BstZ17I GTA'TAC
Cuts at: 0 2674 3924
Size: 2674 1250
Bsu36I CC'TnA_GG
Cuts at: 0 722 3074 3924
Size: 722 2352 850
Fragments arranged by size:
2352 850 722
BtgI C'CryG_G
Cuts at: 0 508 1707 1909 2400 3924
Size: 508 1199 202 491 1524
Fragments arranged by size:
1524 1199 508 491 202
ClaI AT'CG_AT
Cuts at: 0 339 3924
Size: 339 3585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 744 779 1748 1783 3384 3419 3924
Size: 744 35 969 35 1601 35 505
Fragments arranged by size:
1601 969 744 505 35 35 35
DraI TTT'AAA
Cuts at: 0 362 1749 3924
Size: 362 1387 2175
Fragments arranged by size:
2175 1387 362
DraIII CAC_nnn'GTG
Cuts at: 0 3286 3924
Size: 3286 638
DrdI GACnn_nn'nnGTC
Cuts at: 0 531 3924
Size: 531 3393
EaeI y'GGCC_r
Cuts at: 0 582 1219 3924
Size: 582 637 2705
Fragments arranged by size:
2705 637 582
EarI CTCTTCn'nnn_
Cuts at: 0 1269 1435 1987 2026 2149 3023 3924
Size: 1269 166 552 39 123 874 901
Fragments arranged by size:
1269 901 874 552 166 123 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3831 3924
Size: 3831 93
EcoICRI GAG'CTC
Cuts at: 0 110 3924
Size: 110 3814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 316 725 844 1224 1369 1416 1514
Size: 208 108 409 119 380 145 47 98
Cuts at: 1514 1560 1985 2173 2237 2686 3685 3742 3889
Size: 46 425 188 64 449 999 57 147
Cuts at: 3889 3924
Size: 35
Fragments arranged by size:
999 449 425 409 380 208 188 147
145 119 108 98 64 57 47 46
35
EcoO109I rG'GnC_Cy
Cuts at: 0 216 595 718 1125 1540 1717 3924
Size: 216 379 123 407 415 177 2207
Fragments arranged by size:
2207 415 407 379 216 177 123
EcoRI G'AATT_C
Cuts at: 0 2768 3924
Size: 2768 1156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2143 3924
Size: 2143 1781
FspI TGC'GCA
Cuts at: 0 148 3924
Size: 148 3776
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 61 93 659 691 1017 1049
Size: 8 32 21 32 566 32 326 32
Cuts at: 1049 1574 1606 2256 2288 3924
Size: 525 32 650 32 1636
Fragments arranged by size:
1636 650 566 525 326 32 32 32
32 32 32 21 8
HincII GTy'rAC
Cuts at: 0 536 3924
Size: 536 3388
HindIII A'AGCT_T
Cuts at: 0 995 2980 3924
Size: 995 1985 944
Fragments arranged by size:
1985 995 944
KpnI G_GTAC'C
Cuts at: 0 2215 3924
Size: 2215 1709
MfeI C'AATT_G
Cuts at: 0 1139 3924
Size: 1139 2785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 832 2393 2797 2848 3924
Size: 542 290 1561 404 51 1076
Fragments arranged by size:
1561 1076 542 404 290 51
MscI TGG'CCA
Cuts at: 0 1221 3924
Size: 1221 2703
MslI CAynn'nnrTG
Cuts at: 0 1654 2699 3172 3924
Size: 1654 1045 473 752
Fragments arranged by size:
1654 1045 752 473
MspA1I CmG'CkG
Cuts at: 0 1963 2379 3924
Size: 1963 416 1545
Fragments arranged by size:
1963 1545 416
NaeI GCC'GGC
Cuts at: 0 8 3924
Size: 8 3916
NcoI C'CATG_G
Cuts at: 0 508 1707 1909 2400 3924
Size: 508 1199 202 491 1524
Fragments arranged by size:
1524 1199 508 491 202
NdeI CA'TA_TG
Cuts at: 0 852 3924
Size: 852 3072
NgoMIV G'CCGG_C
Cuts at: 0 6 3924
Size: 6 3918
NheI G'CTAG_C
Cuts at: 0 2589 3924
Size: 2589 1335
NsiI A_TGCA'T
Cuts at: 0 3573 3924
Size: 3573 351
NspI r_CATG'y
Cuts at: 0 773 1706 1942 3924
Size: 773 933 236 1982
Fragments arranged by size:
1982 933 773 236
PflMI CCAn_nnn'nTGG
Cuts at: 0 485 1418 3924
Size: 485 933 2506
Fragments arranged by size:
2506 933 485
PfoI T'CCnGG_A
Cuts at: 0 1460 3924
Size: 1460 2464
PpuMI rG'GwC_Cy
Cuts at: 0 216 595 718 1125 1540 1717 3924
Size: 216 379 123 407 415 177 2207
Fragments arranged by size:
2207 415 407 379 216 177 123
PshAI GACnn'nnGTC
Cuts at: 0 1556 3924
Size: 1556 2368
PsiI TTA'TAA
Cuts at: 0 2198 3803 3924
Size: 2198 1605 121
Fragments arranged by size:
2198 1605 121
PstI C_TGCA'G
Cuts at: 0 3779 3924
Size: 3779 145
PvuII CAG'CTG
Cuts at: 0 1963 2379 3924
Size: 1963 416 1545
Fragments arranged by size:
1963 1545 416
SacI G_AGCT'C
Cuts at: 0 112 3924
Size: 112 3812
SanDI GG'GwC_CC
Cuts at: 0 595 1125 3924
Size: 595 530 2799
Fragments arranged by size:
2799 595 530
SapI GCTCTTCn'nnn_
Cuts at: 0 1987 2026 3924
Size: 1987 39 1898
Fragments arranged by size:
1987 1898 39
SbfI CC_TGCA'GG
Cuts at: 0 3779 3924
Size: 3779 145
ScaI AGT'ACT
Cuts at: 0 154 1280 3064 3924
Size: 154 1126 1784 860
Fragments arranged by size:
1784 1126 860 154
SexAI A'CCwGG_T
Cuts at: 0 1256 3924
Size: 1256 2668
SfcI C'TryA_G
Cuts at: 0 366 1284 1333 3240 3775 3924
Size: 366 918 49 1907 535 149
Fragments arranged by size:
1907 918 535 366 149 49
SmlI C'TyrA_G
Cuts at: 0 1309 2055 2933 3924
Size: 1309 746 878 991
Fragments arranged by size:
1309 991 878 746
SpeI A'CTAG_T
Cuts at: 0 1885 3924
Size: 1885 2039
SspI AAT'ATT
Cuts at: 0 356 3796 3924
Size: 356 3440 128
Fragments arranged by size:
3440 356 128
StyI C'CwwG_G
Cuts at: 0 347 508 1707 1866 1909 2169 2276 2400
Size: 347 161 1199 159 43 260 107 124
Cuts at: 2400 3587 3644 3924
Size: 1187 57 280
Fragments arranged by size:
1199 1187 347 280 260 161 159 124
107 57 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 1859 1879 2127 2341 2707 3388 3600
Size: 18 1841 20 248 214 366 681 212
Cuts at: 3600 3779 3924
Size: 179 145
Fragments arranged by size:
1841 681 366 248 214 212 179 145
20 18
TatI w'GTAC_w
Cuts at: 0 152 577 845 1278 1486 1729 2621 3062
Size: 152 425 268 433 208 243 892 441
Cuts at: 3062 3782 3924
Size: 720 142
Fragments arranged by size:
892 720 441 433 425 268 243 208
152 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 621 653 2213 2245 2324 2356 3218 3250
Size: 621 32 1560 32 79 32 862 32
Cuts at: 3250 3338 3370 3556 3588 3924
Size: 88 32 186 32 336
Fragments arranged by size:
1560 862 621 336 186 88 79 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1715 3924
Size: 1715 2209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 819 2283 3924
Size: 819 1464 1641
Fragments arranged by size:
1641 1464 819
XhoI C'TCGA_G
Cuts at: 0 2933 3924
Size: 2933 991
XmnI GAAnn'nnTTC
Cuts at: 0 457 3924
Size: 457 3467
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApoI AvaI BaeI BamHI BanI BanII BbvCI BclI
BfrBI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI Hin4I HincII
HindIII KpnI MfeI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NsiI NspI PflMI PfoI
PpuMI PshAI PsiI PstI PvuII SacI SanDI SapI
SbfI ScaI SexAI SfcI SmlI SpeI SspI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BbeI BbsI BcgI
BciVI BglI BglII BlpI BmgBI BsaHI BsiEI BsiWI
BsmBI BspEI BsrBI BssHII BstBI BtgZI BtsI EagI
EcoNI EcoRV FseI FspAI HaeII HpaI KasI MluI
NarI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI XbaI XmaI ZraI