Restriction map for
pF1KE2301
(Linear) (Six-base) MAPSORT of: KIEE2301.seq Check: 638 from: 1 to: 6531
KIEE2301.orf
With 182 enzymes: SgfI *
August 9, 2016 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3626 5539 5978 6531
Size: 3626 1913 439 553
Fragments arranged by size:
3626 1913 553 439
AccI GT'mk_AC
Cuts at: 0 1840 4709 5028 6531
Size: 1840 2869 319 1503
Fragments arranged by size:
2869 1840 1503 319
Acc65I G'GTAC_C
Cuts at: 0 5881 6531
Size: 5881 650
AclI AA'CG_TT
Cuts at: 0 3882 6531
Size: 3882 2649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 201 1401 1967 2480 2498 2961 6409
Size: 61 140 1200 566 513 18 463 3448
Cuts at: 6409 6531
Size: 122
Fragments arranged by size:
3448 1200 566 513 463 140 122 61
18
AfeI AGC'GCT
Cuts at: 0 2905 4264 6531
Size: 2905 1359 2267
Fragments arranged by size:
2905 2267 1359
AflII C'TTAA_G
Cuts at: 0 3286 3889 6531
Size: 3286 603 2642
Fragments arranged by size:
3286 2642 603
AflIII A'CryG_T
Cuts at: 0 534 1835 2190 4323 4459 4790 4948 6531
Size: 534 1301 355 2133 136 331 158 1583
Fragments arranged by size:
2133 1583 1301 534 355 331 158 136
AgeI A'CCGG_T
Cuts at: 0 2879 6531
Size: 2879 3652
AhdI GACnn_n'nnGTC
Cuts at: 0 3818 4716 6531
Size: 3818 898 1815
Fragments arranged by size:
3818 1815 898
AleI CACnn'nnGTG
Cuts at: 0 3182 4741 6531
Size: 3182 1559 1790
Fragments arranged by size:
3182 1790 1559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 188 1268 2816 3934 5723 6531
Size: 188 1080 1548 1118 1789 808
Fragments arranged by size:
1789 1548 1118 1080 808 188
AlwNI CAG_nnn'CTG
Cuts at: 0 454 625 1719 2368 2688 3857 4368 4914
Size: 454 171 1094 649 320 1169 511 546
Cuts at: 4914 5601 5875 6060 6261 6531
Size: 687 274 185 201 270
Fragments arranged by size:
1169 1094 687 649 546 511 454 320
274 270 201 185 171
ApaI G_GGCC'C
Cuts at: 0 662 3507 5416 6531
Size: 662 2845 1909 1115
Fragments arranged by size:
2845 1909 1115 662
ApaLI G'TGCA_C
Cuts at: 0 4519 5995 6531
Size: 4519 1476 536
Fragments arranged by size:
4519 1476 536
ApoI r'AATT_y
Cuts at: 0 856 1774 2962 6531
Size: 856 918 1188 3569
Fragments arranged by size:
3569 1188 918 856
AvaI C'yCGr_G
Cuts at: 0 1859 4934 5144 5415 6531
Size: 1859 3075 210 271 1116
Fragments arranged by size:
3075 1859 1116 271 210
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5873 5906 6531
Size: 5873 33 625
Fragments arranged by size:
5873 625 33
BanI G'GyrC_C
Cuts at: 0 364 3051 3191 5712 5881 5889 6531
Size: 364 2687 140 2521 169 8 642
Fragments arranged by size:
2687 2521 642 364 169 140 8
BanII G_rGCy'C
Cuts at: 0 662 1884 1985 2975 3507 4733 4966 5297
Size: 662 1222 101 990 532 1226 233 331
Cuts at: 5297 5416 6531
Size: 119 1115
Fragments arranged by size:
1226 1222 1115 990 662 532 331 233
119 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 4067 6531
Size: 4067 2464
BbvCI CC'TCA_GC
Cuts at: 0 1001 1058 1084 6531
Size: 1001 57 26 5447
Fragments arranged by size:
5447 1001 57 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4839 4873 6531
Size: 4839 34 1658
Fragments arranged by size:
4839 1658 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1102 1716 2920 6531
Size: 1102 614 1204 3611
Fragments arranged by size:
3611 1204 1102 614
BclI T'GATC_A
Cuts at: 0 4097 6531
Size: 4097 2434
BfrBI ATG'CAT
Cuts at: 0 4587 6531
Size: 4587 1944
BglII A'GATC_T
Cuts at: 0 1484 3432 5051 6531
Size: 1484 1948 1619 1480
Fragments arranged by size:
1948 1619 1484 1480
BlpI GC'TnA_GC
Cuts at: 0 1613 2714 4249 4756 6531
Size: 1613 1101 1535 507 1775
Fragments arranged by size:
1775 1613 1535 1101 507
Bme1580I G_kGCm'C
Cuts at: 0 662 1057 3054 3194 3507 3945 4523 5416
Size: 662 395 1997 140 313 438 578 893
Cuts at: 5416 5717 5894 5999 6105 6531
Size: 301 177 105 106 426
Fragments arranged by size:
1997 893 662 578 438 426 395 313
301 177 140 106 105
BmgBI CAC'GTC
Cuts at: 0 3180 6531
Size: 3180 3351
BmrI ACTGGGnnnn_n'
Cuts at: 0 1676 3922 5324 5978 6387 6399 6500 6531
Size: 1676 2246 1402 654 409 12 101 31
Fragments arranged by size:
2246 1676 1402 654 409 101 31 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1506 4143 6531
Size: 1506 2637 2388
Fragments arranged by size:
2637 2388 1506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1606 2423 2578 4027 4705 5236 5273
Size: 821 785 817 155 1449 678 531 37
Cuts at: 5273 5645 5728 5889 6531
Size: 372 83 161 642
Fragments arranged by size:
1449 821 817 785 678 642 531 372
161 155 83 37
Bpu10I CC'TnA_GC
Cuts at: 0 1001 1058 1084 2537 6146 6531
Size: 1001 57 26 1453 3609 385
Fragments arranged by size:
3609 1453 1001 385 57 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 796 3655 6531
Size: 39 757 2859 2876
Fragments arranged by size:
2876 2859 757 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 508 2399 2547 6531
Size: 508 1891 148 3984
Fragments arranged by size:
3984 1891 508 148
BsaAI yAC'GTr
Cuts at: 0 2193 3843 4949 6531
Size: 2193 1650 1106 1582
Fragments arranged by size:
2193 1650 1582 1106
BsaBI GATnn'nnATC
Cuts at: 0 4693 6531
Size: 4693 1838
BsaHI Gr'CG_yC
Cuts at: 0 2858 5651 6110 6122 6531
Size: 2858 2793 459 12 409
Fragments arranged by size:
2858 2793 459 409 12
BsaWI w'CCGG_w
Cuts at: 0 2879 5451 6531
Size: 2879 2572 1080
Fragments arranged by size:
2879 2572 1080
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 981 1011 1716 1746 3774 3804 4867 4897
Size: 981 30 705 30 2028 30 1063 30
Cuts at: 4897 5433 5463 6075 6105 6531
Size: 536 30 612 30 426
Fragments arranged by size:
2028 1063 981 705 612 536 426 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 989 1479 2242 2883 3294 3328 3591 3766
Size: 989 490 763 641 411 34 263 175
Cuts at: 3766 3769 3776 4669 5162 5167 5260 5661 6365
Size: 3 7 893 493 5 93 401 704
Cuts at: 6365 6531
Size: 166
Fragments arranged by size:
989 893 763 704 641 493 490 411
401 263 175 166 93 34 7 5
3
BseYI C'CCAG_C
Cuts at: 0 54 3047 4016 4222 4339 4652 5794 5896
Size: 54 2993 969 206 117 313 1142 102
Cuts at: 5896 6386 6398 6434 6494 6531
Size: 490 12 36 60 37
Fragments arranged by size:
2993 1142 969 490 313 206 117 102
60 54 37 36 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 2215 2629 2638 3254 3287 4660 5446
Size: 302 1913 414 9 616 33 1373 786
Cuts at: 5446 6531
Size: 1085
Fragments arranged by size:
1913 1373 1085 786 616 414 302 33
9
BsiEI CG_ry'CG
Cuts at: 0 49 3612 6531
Size: 49 3563 2919
Fragments arranged by size:
3563 2919 49
BsiHKAI G_wGCw'C
Cuts at: 0 1689 1937 2975 4250 4523 4733 4766 5926
Size: 1689 248 1038 1275 273 210 33 1160
Cuts at: 5926 5999 6531
Size: 73 532
Fragments arranged by size:
1689 1275 1160 1038 532 273 248 210
73 33
BsmI GAATG_Cn'
Cuts at: 0 746 4037 6531
Size: 746 3291 2494
Fragments arranged by size:
3291 2494 746
Bsp1286I G_dGCh'C
Cuts at: 0 662 1057 1689 1884 1937 1985 2975 3054
Size: 662 395 632 195 53 48 990 79
Cuts at: 3054 3194 3507 3945 4250 4523 4733 4766 4966
Size: 140 313 438 305 273 210 33 200
Cuts at: 4966 5297 5416 5717 5894 5926 5999 6105 6531
Size: 331 119 301 177 32 73 106 426
Fragments arranged by size:
990 662 632 438 426 395 331 313
305 301 273 210 200 195 177 140
119 106 79 73 53 48 33 32
BspEI T'CCGG_A
Cuts at: 0 5451 6531
Size: 5451 1080
BspHI T'CATG_A
Cuts at: 0 3062 3372 4094 6531
Size: 3062 310 722 2437
Fragments arranged by size:
3062 2437 722 310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1052 1217 1236 3523 3626 3926 5539 5553
Size: 1052 165 19 2287 103 300 1613 14
Cuts at: 5553 5715 5869 5978 6531
Size: 162 154 109 553
Fragments arranged by size:
2287 1613 1052 553 300 165 162 154
109 103 19 14
BsrBI CCG'CTC
Cuts at: 0 1290 1767 3599 5426 6531
Size: 1290 477 1832 1827 1105
Fragments arranged by size:
1832 1827 1290 1105 477
BsrDI GCAATG_nn'
Cuts at: 0 1513 3171 3351 3918 4292 6531
Size: 1513 1658 180 567 374 2239
Fragments arranged by size:
2239 1658 1513 567 374 180
BsrFI r'CCGG_y
Cuts at: 0 32 614 884 1064 1168 2453 2879 3244
Size: 32 582 270 180 104 1285 426 365
Cuts at: 3244 5132 5693 6531
Size: 1888 561 838
Fragments arranged by size:
1888 1285 838 582 561 426 365 270
180 104 32
BsrGI T'GTAC_A
Cuts at: 0 901 2933 5522 6531
Size: 901 2032 2589 1009
Fragments arranged by size:
2589 2032 1009 901
BssSI C'ACGA_G
Cuts at: 0 2568 5558 6531
Size: 2568 2990 973
Fragments arranged by size:
2990 2568 973
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2616 3420 3857 4584 5166 5601 6261 6531
Size: 2616 804 437 727 582 435 660 270
Fragments arranged by size:
2616 804 727 660 582 437 435 270
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1584 3571 5102 6531
Size: 1584 1987 1531 1429
Fragments arranged by size:
1987 1584 1531 1429
BstYI r'GATC_y
Cuts at: 0 14 791 1484 1974 2736 3432 5051 5064
Size: 14 777 693 490 762 696 1619 13
Cuts at: 5064 5955 6257 6531
Size: 891 302 274
Fragments arranged by size:
1619 891 777 762 696 693 490 302
274 14 13
BstZ17I GTA'TAC
Cuts at: 0 1841 6531
Size: 1841 4690
Bsu36I CC'TnA_GG
Cuts at: 0 77 5188 6531
Size: 77 5111 1343
Fragments arranged by size:
5111 1343 77
BtgI C'CryG_G
Cuts at: 0 49 4661 5885 6531
Size: 49 4612 1224 646
Fragments arranged by size:
4612 1224 646 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1641 3204 3390 4128 6531
Size: 1641 1563 186 738 2403
Fragments arranged by size:
2403 1641 1563 738 186
BtsI GCAGTG_nn'
Cuts at: 0 177 700 2471 3421 3777 4918 5221 5227
Size: 177 523 1771 950 356 1141 303 6
Cuts at: 5227 6058 6531
Size: 831 473
Fragments arranged by size:
1771 1141 950 831 523 473 356 303
177 6
ClaI AT'CG_AT
Cuts at: 0 4973 6531
Size: 4973 1558
DraI TTT'AAA
Cuts at: 0 2954 6531
Size: 2954 3577
DraIII CAC_nnn'GTG
Cuts at: 0 3265 4667 6531
Size: 3265 1402 1864
Fragments arranged by size:
3265 1864 1402
EaeI y'GGCC_r
Cuts at: 0 46 616 716 3792 6531
Size: 46 570 100 3076 2739
Fragments arranged by size:
3076 2739 570 100 46
EagI C'GGCC_G
Cuts at: 0 46 6531
Size: 46 6485
EarI CTCTTCn'nnn_
Cuts at: 0 413 523 1585 2980 4383 4405 4738 5805
Size: 413 110 1062 1395 1403 22 333 1067
Cuts at: 5805 6531
Size: 726
Fragments arranged by size:
1403 1395 1067 1062 726 413 333 110
22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 784 1615 6531
Size: 784 831 4916
Fragments arranged by size:
4916 831 784
EcoICRI GAG'CTC
Cuts at: 0 2973 4731 6531
Size: 2973 1758 1800
Fragments arranged by size:
2973 1800 1758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 201 821 1401 1606 1967 2423 2480
Size: 61 140 620 580 205 361 456 57
Cuts at: 2480 2498 2578 2961 4027 4705 5236 5273 5645
Size: 18 80 383 1066 678 531 37 372
Cuts at: 5645 5728 5889 6409 6531
Size: 83 161 520 122
Fragments arranged by size:
1066 678 620 580 531 520 456 383
372 361 205 161 140 122 83 80
61 57 37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2818 3587 6531
Size: 2818 769 2944
Fragments arranged by size:
2944 2818 769
EcoO109I rG'GnC_Cy
Cuts at: 0 58 81 396 622 658 659 1462 1866
Size: 58 23 315 226 36 1 803 404
Cuts at: 1866 2159 3503 5192 5872 6006 6531
Size: 293 1344 1689 680 134 525
Fragments arranged by size:
1689 1344 803 680 525 404 315 293
226 134 58 36 23 1
EcoRI G'AATT_C
Cuts at: 0 1774 6531
Size: 1774 4757
EcoRV GAT'ATC
Cuts at: 0 2331 2484 6531
Size: 2331 153 4047
Fragments arranged by size:
4047 2331 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 318 2179 2349 6531
Size: 318 1861 170 4182
Fragments arranged by size:
4182 1861 318 170
FspI TGC'GCA
Cuts at: 0 3850 6531
Size: 3850 2681
HaeII r_GCGC'y
Cuts at: 0 2907 3957 4266 4881 5730 6417 6531
Size: 2907 1050 309 615 849 687 114
Fragments arranged by size:
2907 1050 849 687 615 309 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1734 1766 1810 1842 2878 2910 2974 3006
Size: 1734 32 44 32 1036 32 64 32
Cuts at: 3006 3057 3089 3804 3836 4174 4206 4702 4734
Size: 51 32 715 32 338 32 496 32
Cuts at: 4734 4867 4899 4928 4960 5433 5465 6021 6053
Size: 133 32 29 32 473 32 556 32
Cuts at: 6053 6531
Size: 478
Fragments arranged by size:
1734 1036 715 556 496 478 473 338
133 64 51 44 32 32 32 32
32 32 32 32 32 32 32 32
29
HincII GTy'rAC
Cuts at: 0 540 971 4710 6531
Size: 540 431 3739 1821
Fragments arranged by size:
3739 1821 540 431
KpnI G_GTAC'C
Cuts at: 0 5885 6531
Size: 5885 646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1229 3760 4194 4727 4745 5401 6531
Size: 236 993 2531 434 533 18 656 1130
Fragments arranged by size:
2531 1130 993 656 533 434 236 18
MscI TGG'CCA
Cuts at: 0 718 6531
Size: 718 5813
MslI CAynn'nnrTG
Cuts at: 0 170 481 724 1518 2798 3182 3805 4741
Size: 170 311 243 794 1280 384 623 936
Cuts at: 4741 5641 6531
Size: 900 890
Fragments arranged by size:
1280 936 900 890 794 623 384 311
243 170
MspA1I CmG'CkG
Cuts at: 0 51 924 1088 1538 2256 2685 4020 4700
Size: 51 873 164 450 718 429 1335 680
Cuts at: 4700 4911 5163 5979 6531
Size: 211 252 816 552
Fragments arranged by size:
1335 873 816 718 680 552 450 429
252 211 164 51
NaeI GCC'GGC
Cuts at: 0 886 6531
Size: 886 5645
NcoI C'CATG_G
Cuts at: 0 5885 6531
Size: 5885 646
NgoMIV G'CCGG_C
Cuts at: 0 884 6531
Size: 884 5647
NotI GC'GGCC_GC
Cuts at: 0 46 6531
Size: 46 6485
NsiI A_TGCA'T
Cuts at: 0 4589 6531
Size: 4589 1942
NspI r_CATG'y
Cuts at: 0 538 1839 2589 2617 4221 4327 4419 4463
Size: 538 1301 750 28 1604 106 92 44
Cuts at: 4463 4629 4716 4755 4794 5763 6531
Size: 166 87 39 39 969 768
Fragments arranged by size:
1604 1301 969 768 750 538 166 106
92 87 44 39 39 28
PasI CC'CwG_GG
Cuts at: 0 5060 5823 6105 6531
Size: 5060 763 282 426
Fragments arranged by size:
5060 763 426 282
PciI A'CATG_T
Cuts at: 0 534 1835 4323 4459 4790 6531
Size: 534 1301 2488 136 331 1741
Fragments arranged by size:
2488 1741 1301 534 331 136
PflMI CCAn_nnn'nTGG
Cuts at: 0 2259 3621 4050 4667 5476 6531
Size: 2259 1362 429 617 809 1055
Fragments arranged by size:
2259 1362 1055 809 617 429
PfoI T'CCnGG_A
Cuts at: 0 1583 1697 2400 2545 3425 4682 6531
Size: 1583 114 703 145 880 1257 1849
Fragments arranged by size:
1849 1583 1257 880 703 145 114
PmlI CAC'GTG
Cuts at: 0 2193 4949 6531
Size: 2193 2756 1582
Fragments arranged by size:
2756 2193 1582
PpuMI rG'GwC_Cy
Cuts at: 0 81 396 1462 1866 2159 6531
Size: 81 315 1066 404 293 4372
Fragments arranged by size:
4372 1066 404 315 293 81
PshAI GACnn'nnGTC
Cuts at: 0 266 5650 6531
Size: 266 5384 881
Fragments arranged by size:
5384 881 266
PsiI TTA'TAA
Cuts at: 0 5678 6531
Size: 5678 853
PspOMI G'GGCC_C
Cuts at: 0 658 3503 5412 6531
Size: 658 2845 1909 1119
Fragments arranged by size:
2845 1909 1119 658
PspXI vC'TCGA_Gb
Cuts at: 0 5144 6531
Size: 5144 1387
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2995 3027 6531
Size: 2995 32 3504
Fragments arranged by size:
3504 2995 32
PstI C_TGCA'G
Cuts at: 0 630 923 1347 1715 2901 3090 4091 4788
Size: 630 293 424 368 1186 189 1001 697
Cuts at: 4788 5550 5880 6065 6531
Size: 762 330 185 466
Fragments arranged by size:
1186 1001 762 697 630 466 424 368
330 293 189 185
PvuII CAG'CTG
Cuts at: 0 924 1088 1538 2685 4911 6531
Size: 924 164 450 1147 2226 1620
Fragments arranged by size:
2226 1620 1147 924 450 164
RsrII CG'GwC_CG
Cuts at: 0 2882 6531
Size: 2882 3649
SacI G_AGCT'C
Cuts at: 0 2975 4733 6531
Size: 2975 1758 1798
Fragments arranged by size:
2975 1798 1758
SacII CC_GC'GG
Cuts at: 0 52 6531
Size: 52 6479
SalI G'TCGA_C
Cuts at: 0 4708 6531
Size: 4708 1823
SanDI GG'GwC_CC
Cuts at: 0 2159 6531
Size: 2159 4372
SapI GCTCTTCn'nnn_
Cuts at: 0 2980 4405 4738 5805 6531
Size: 2980 1425 333 1067 726
Fragments arranged by size:
2980 1425 1067 726 333
SbfI CC_TGCA'GG
Cuts at: 0 630 5550 5880 6531
Size: 630 4920 330 651
Fragments arranged by size:
4920 651 630 330
ScaI AGT'ACT
Cuts at: 0 577 1790 4206 4555 5334 6531
Size: 577 1213 2416 349 779 1197
Fragments arranged by size:
2416 1213 1197 779 577 349
SexAI A'CCwGG_T
Cuts at: 0 2525 4613 6531
Size: 2525 2088 1918
Fragments arranged by size:
2525 2088 1918
SfcI C'TryA_G
Cuts at: 0 626 919 1096 1343 1711 2897 2943 3086
Size: 626 293 177 247 368 1186 46 143
Cuts at: 3086 3150 3584 4087 4509 4784 5029 5499 5546
Size: 64 434 503 422 275 245 470 47
Cuts at: 5546 5876 6061 6531
Size: 330 185 470
Fragments arranged by size:
1186 626 503 470 470 434 422 368
330 293 275 247 245 185 177 143
64 47 46
SmaI CCC'GGG
Cuts at: 0 5417 6531
Size: 5417 1114
SmlI C'TyrA_G
Cuts at: 0 18 775 3286 3670 3889 5144 6531
Size: 18 757 2511 384 219 1255 1387
Fragments arranged by size:
2511 1387 1255 757 384 219 18
SspI AAT'ATT
Cuts at: 0 2644 6531
Size: 2644 3887
StuI AGG'CCT
Cuts at: 0 3292 5519 6531
Size: 3292 2227 1012
Fragments arranged by size:
3292 2227 1012
StyI C'CwwG_G
Cuts at: 0 518 1127 2141 2163 2324 2580 4079 4148
Size: 518 609 1014 22 161 256 1499 69
Cuts at: 4148 4979 5360 5718 5885 6531
Size: 831 381 358 167 646
Fragments arranged by size:
1499 1014 831 646 609 518 381 358
256 167 161 69 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 759 2154 2221 4666 5343 5483 5542 5941
Size: 759 1395 67 2445 677 140 59 399
Cuts at: 5941 6087 6531
Size: 146 444
Fragments arranged by size:
2445 1395 759 677 444 399 146 140
67 59
TatI w'GTAC_w
Cuts at: 0 575 901 1788 2933 3684 4204 4553 5332
Size: 575 326 887 1145 751 520 349 779
Cuts at: 5332 5522 6531
Size: 190 1009
Fragments arranged by size:
1145 1009 887 779 751 575 520 349
326 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 981 1013 2196 2228 3774 3806 4357 4389
Size: 981 32 1183 32 1546 32 551 32
Cuts at: 4389 6531
Size: 2142
Fragments arranged by size:
2142 1546 1183 981 551 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 4188 6531
Size: 4188 2343
XbaI T'CTAG_A
Cuts at: 0 3075 4257 6531
Size: 3075 1182 2274
Fragments arranged by size:
3075 2274 1182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4680 5473 6531
Size: 4680 793 1058
Fragments arranged by size:
4680 1058 793
XhoI C'TCGA_G
Cuts at: 0 5144 6531
Size: 5144 1387
XmaI C'CCGG_G
Cuts at: 0 5415 6531
Size: 5415 1116
XmnI GAAnn'nnTTC
Cuts at: 0 860 1808 3743 4901 6531
Size: 860 948 1935 1158 1630
Fragments arranged by size:
1935 1630 1158 948 860
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NotI NsiI NspI PasI PciI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SmaI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AloI AscI AseI AsiSI AvrII BamHI BbeI
BglI BmtI BsiWI BsmBI BssHII BstBI BstEII CspCI
DrdI FseI FspAI HindIII HpaI KasI MfeI MluI
NarI NdeI NheI NruI PacI PmeI PpiI PvuI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI ZraI