Restriction map for
pF1KE1993
(Linear) (Six-base) MAPSORT of: KIEE1993.seq Check: 1136 from: 1 to: 1716
KIEE1993.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AatII G_ACGT'C
Cuts at: 0 215 253 1564 1716
Size: 215 38 1311 152
Fragments arranged by size:
1311 215 152 38
AfeI AGC'GCT
Cuts at: 0 461 1716
Size: 461 1255
AflIII A'CryG_T
Cuts at: 0 666 1716
Size: 666 1050
AhdI GACnn_n'nnGTC
Cuts at: 0 387 1716
Size: 387 1329
AleI CACnn'nnGTG
Cuts at: 0 760 1716
Size: 760 956
ApaI G_GGCC'C
Cuts at: 0 658 1716
Size: 658 1058
ApoI r'AATT_y
Cuts at: 0 483 1716
Size: 483 1233
AvaI C'yCGr_G
Cuts at: 0 658 1285 1716
Size: 658 627 431
Fragments arranged by size:
658 627 431
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1425 1458 1716
Size: 1425 33 258
Fragments arranged by size:
1425 258 33
BamHI G'GATC_C
Cuts at: 0 632 1210 1716
Size: 632 578 506
Fragments arranged by size:
632 578 506
BanI G'GyrC_C
Cuts at: 0 574 1060 1369 1677 1716
Size: 574 486 309 308 39
Fragments arranged by size:
574 486 309 308 39
BanII G_rGCy'C
Cuts at: 0 658 833 925 1716
Size: 658 175 92 791
Fragments arranged by size:
791 658 175 92
BbeI G_GCGC'C
Cuts at: 0 578 1681 1716
Size: 578 1103 35
Fragments arranged by size:
1103 578 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 40 172 1164 1716
Size: 40 132 992 552
Fragments arranged by size:
992 552 132 40
BbvCI CC'TCA_GC
Cuts at: 0 782 1716
Size: 782 934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 595 629 1716
Size: 595 34 1087
Fragments arranged by size:
1087 595 34
BclI T'GATC_A
Cuts at: 0 53 122 237 308 1716
Size: 53 69 115 71 1408
Fragments arranged by size:
1408 115 71 69 53
BglI GCCn_nnn'nGGC
Cuts at: 0 1021 1552 1716
Size: 1021 531 164
Fragments arranged by size:
1021 531 164
BlpI GC'TnA_GC
Cuts at: 0 673 1466 1716
Size: 673 793 250
Fragments arranged by size:
793 673 250
Bme1580I G_kGCm'C
Cuts at: 0 658 996 1063 1716
Size: 658 338 67 653
Fragments arranged by size:
658 653 338 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 891 1124 1716
Size: 891 233 592
Fragments arranged by size:
891 592 233
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 1716
Size: 198 1518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 923 961 1381 1526 1716
Size: 891 32 38 420 145 190
Fragments arranged by size:
891 420 190 145 38 32
Bpu10I CC'TnA_GC
Cuts at: 0 329 782 1716
Size: 329 453 934
Fragments arranged by size:
934 453 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 939 1716
Size: 939 777
BsaI GGTCTCn'nnnn_
Cuts at: 0 965 1716
Size: 965 751
BsaBI GATnn'nnATC
Cuts at: 0 193 1716
Size: 193 1523
BsaHI Gr'CG_yC
Cuts at: 0 212 250 575 1525 1561 1678 1716
Size: 212 38 325 950 36 117 38
Fragments arranged by size:
950 325 212 117 38 38 36
BsaWI w'CCGG_w
Cuts at: 0 1053 1592 1716
Size: 1053 539 124
Fragments arranged by size:
1053 539 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1069 1099 1524 1554 1716
Size: 1069 30 425 30 162
Fragments arranged by size:
1069 425 162 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 173 463 634 700 1090 1539 1716
Size: 173 290 171 66 390 449 177
Fragments arranged by size:
449 390 290 177 173 171 66
BseYI C'CCAG_C
Cuts at: 0 292 1664 1716
Size: 292 1372 52
Fragments arranged by size:
1372 292 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 1716
Size: 1585 131
BsiEI CG_ry'CG
Cuts at: 0 45 1716
Size: 45 1671
BsiHKAI G_wGCw'C
Cuts at: 0 1632 1716
Size: 1632 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1568 1716
Size: 1568 148
Bsp1286I G_dGCh'C
Cuts at: 0 658 833 925 996 1063 1632 1716
Size: 658 175 92 71 67 569 84
Fragments arranged by size:
658 569 175 92 84 71 67
BspEI T'CCGG_A
Cuts at: 0 1053 1592 1716
Size: 1053 539 124
Fragments arranged by size:
1053 539 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1607 1716
Size: 1607 109
BsrBI CCG'CTC
Cuts at: 0 1080 1716
Size: 1080 636
BsrDI GCAATG_nn'
Cuts at: 0 611 996 1199 1716
Size: 611 385 203 517
Fragments arranged by size:
611 517 385 203
BsrGI T'GTAC_A
Cuts at: 0 497 1716
Size: 497 1219
BssSI C'ACGA_G
Cuts at: 0 36 493 1484 1716
Size: 36 457 991 232
Fragments arranged by size:
991 457 232 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 392 1313 1716
Size: 392 921 403
Fragments arranged by size:
921 403 392
BstYI r'GATC_y
Cuts at: 0 632 639 1210 1716
Size: 632 7 571 506
Fragments arranged by size:
632 571 506 7
Bsu36I CC'TnA_GG
Cuts at: 0 683 1716
Size: 683 1033
BtgI C'CryG_G
Cuts at: 0 1317 1716
Size: 1317 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 708 774 1716
Size: 708 66 942
Fragments arranged by size:
942 708 66
BtsI GCAGTG_nn'
Cuts at: 0 836 996 1010 1716
Size: 836 160 14 706
Fragments arranged by size:
836 706 160 14
DrdI GACnn_nn'nnGTC
Cuts at: 0 979 1716
Size: 979 737
EaeI y'GGCC_r
Cuts at: 0 1553 1689 1716
Size: 1553 136 27
Fragments arranged by size:
1553 136 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 923 961 1381 1526 1716
Size: 891 32 38 420 145 190
Fragments arranged by size:
891 420 190 145 38 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1190 1716
Size: 1190 526
EcoO109I rG'GnC_Cy
Cuts at: 0 655 1236 1581 1716
Size: 655 581 345 135
Fragments arranged by size:
655 581 345 135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1056 1716
Size: 1056 660
HaeII r_GCGC'y
Cuts at: 0 463 578 1681 1716
Size: 463 115 1103 35
Fragments arranged by size:
1103 463 115 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 62 70 102 222 254 373 405
Size: 30 32 8 32 120 32 119 32
Cuts at: 405 624 656 912 944 1069 1101 1524 1556
Size: 219 32 256 32 125 32 423 32
Cuts at: 1556 1716
Size: 160
Fragments arranged by size:
423 256 219 160 125 120 119 32
32 32 32 32 32 32 32 30
8
KasI G'GCGC_C
Cuts at: 0 574 1677 1716
Size: 574 1103 39
Fragments arranged by size:
1103 574 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1716
Size: 955 761
MslI CAynn'nnrTG
Cuts at: 0 760 1204 1716
Size: 760 444 512
Fragments arranged by size:
760 512 444
MspA1I CmG'CkG
Cuts at: 0 747 1319 1716
Size: 747 572 397
Fragments arranged by size:
747 572 397
NarI GG'CG_CC
Cuts at: 0 575 1678 1716
Size: 575 1103 38
Fragments arranged by size:
1103 575 38
NspI r_CATG'y
Cuts at: 0 415 670 1381 1716
Size: 415 255 711 335
Fragments arranged by size:
711 415 335 255
PciI A'CATG_T
Cuts at: 0 666 1716
Size: 666 1050
PflMI CCAn_nnn'nTGG
Cuts at: 0 1140 1716
Size: 1140 576
PfoI T'CCnGG_A
Cuts at: 0 184 443 563 635 1358 1716
Size: 184 259 120 72 723 358
Fragments arranged by size:
723 358 259 184 120 72
PpuMI rG'GwC_Cy
Cuts at: 0 1236 1716
Size: 1236 480
PspOMI G'GGCC_C
Cuts at: 0 654 1716
Size: 654 1062
PvuI CG_AT'CG
Cuts at: 0 45 1716
Size: 45 1671
SacII CC_GC'GG
Cuts at: 0 1320 1716
Size: 1320 396
SanDI GG'GwC_CC
Cuts at: 0 1236 1716
Size: 1236 480
ScaI AGT'ACT
Cuts at: 0 551 1716
Size: 551 1165
SexAI A'CCwGG_T
Cuts at: 0 1223 1716
Size: 1223 493
SfcI C'TryA_G
Cuts at: 0 1025 1716
Size: 1025 691
SfoI GGC'GCC
Cuts at: 0 576 1679 1716
Size: 576 1103 37
Fragments arranged by size:
1103 576 37
SmlI C'TyrA_G
Cuts at: 0 918 1285 1716
Size: 918 367 431
Fragments arranged by size:
918 431 367
SspI AAT'ATT
Cuts at: 0 24 1716
Size: 24 1692
StyI C'CwwG_G
Cuts at: 0 270 278 758 887 1716
Size: 270 8 480 129 829
Fragments arranged by size:
829 480 270 129 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1678 1716
Size: 1678 38
TatI w'GTAC_w
Cuts at: 0 497 549 1289 1716
Size: 497 52 740 427
Fragments arranged by size:
740 497 427 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 1035 1067 1716
Size: 803 32 200 32 649
Fragments arranged by size:
803 649 200 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1225 1716
Size: 1225 491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 598 1716
Size: 285 313 1118
Fragments arranged by size:
1118 313 285
XhoI C'TCGA_G
Cuts at: 0 1285 1716
Size: 1285 431
XmnI GAAnn'nnTTC
Cuts at: 0 1417 1716
Size: 1417 299
ZraI GAC'GTC
Cuts at: 0 213 251 1562 1716
Size: 213 38 1311 154
Fragments arranged by size:
1311 213 154 38
Enzymes that do cut:
AatII AfeI AflIII AhdI AleI ApaI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MmeI MslI MspA1I NarI NspI PciI
PflMI PfoI PpuMI PspOMI PvuI SacII SanDI ScaI
SexAI SfcI SfoI SmlI SspI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AgeI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BciVI
BfrBI BglII BmgBI BmtI BsaAI BsiWI BsmI BspHI
BsrFI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI
DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuII RsrII SacI SalI
SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI XbaI XmaI