Restriction map for
pF1KE1875
(Linear) (Six-base) MAPSORT of: KIEE1875.seq Check: 1052 from: 1 to: 1194
KIEE1875.orf
With 182 enzymes: SgfI *
July 27, 2017 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 733 1194
Size: 733 461
AccI GT'mk_AC
Cuts at: 0 1159 1194
Size: 1159 35
AlwNI CAG_nnn'CTG
Cuts at: 0 512 618 1134 1194
Size: 512 106 516 60
Fragments arranged by size:
516 512 106 60
ApaI G_GGCC'C
Cuts at: 0 246 418 1194
Size: 246 172 776
Fragments arranged by size:
776 246 172
AvaI C'yCGr_G
Cuts at: 0 285 1194
Size: 285 909
BanI G'GyrC_C
Cuts at: 0 265 1194
Size: 265 929
BanII G_rGCy'C
Cuts at: 0 230 246 418 458 1194
Size: 230 16 172 40 736
Fragments arranged by size:
736 230 172 40 16
BbeI G_GCGC'C
Cuts at: 0 269 1194
Size: 269 925
BglI GCCn_nnn'nGGC
Cuts at: 0 290 1194
Size: 290 904
BlpI GC'TnA_GC
Cuts at: 0 674 1194
Size: 674 520
Bme1580I G_kGCm'C
Cuts at: 0 246 359 418 1194
Size: 246 113 59 776
Fragments arranged by size:
776 246 113 59
BmgBI CAC'GTC
Cuts at: 0 744 1194
Size: 744 450
BmrI ACTGGGnnnn_n'
Cuts at: 0 298 346 592 1194
Size: 298 48 246 602
Fragments arranged by size:
602 298 246 48
BmtI G_CTAG'C
Cuts at: 0 335 1194
Size: 335 859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 472 1194
Size: 282 190 722
Fragments arranged by size:
722 282 190
Bpu10I CC'TnA_GC
Cuts at: 0 22 139 1194
Size: 22 117 1055
Fragments arranged by size:
1055 117 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1014 1194
Size: 556 458 180
Fragments arranged by size:
556 458 180
BsaHI Gr'CG_yC
Cuts at: 0 104 266 1194
Size: 104 162 928
Fragments arranged by size:
928 162 104
BsaWI w'CCGG_w
Cuts at: 0 230 235 1194
Size: 230 5 959
Fragments arranged by size:
959 230 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 248 261 409 512 670 947 1194
Size: 248 13 148 103 158 277 247
Fragments arranged by size:
277 248 247 158 148 103 13
BseYI C'CCAG_C
Cuts at: 0 275 926 1194
Size: 275 651 268
Fragments arranged by size:
651 275 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1194
Size: 79 1115
BsiEI CG_ry'CG
Cuts at: 0 250 495 1194
Size: 250 245 699
Fragments arranged by size:
699 250 245
BsiHKAI G_wGCw'C
Cuts at: 0 230 458 569 1194
Size: 230 228 111 625
Fragments arranged by size:
625 230 228 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 522 1194
Size: 522 672
Bsp1286I G_dGCh'C
Cuts at: 0 230 246 359 418 458 569 1194
Size: 230 16 113 59 40 111 625
Fragments arranged by size:
625 230 113 111 59 40 16
BspEI T'CCGG_A
Cuts at: 0 230 1194
Size: 230 964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 1194
Size: 733 461
BsrBI CCG'CTC
Cuts at: 0 18 26 1194
Size: 18 8 1168
Fragments arranged by size:
1168 18 8
BsrDI GCAATG_nn'
Cuts at: 0 821 1194
Size: 821 373
BsrFI r'CCGG_y
Cuts at: 0 557 1194
Size: 557 637
BsrGI T'GTAC_A
Cuts at: 0 1140 1194
Size: 1140 54
BssSI C'ACGA_G
Cuts at: 0 76 457 1194
Size: 76 381 737
Fragments arranged by size:
737 381 76
BstZ17I GTA'TAC
Cuts at: 0 1160 1194
Size: 1160 34
Bsu36I CC'TnA_GG
Cuts at: 0 392 1194
Size: 392 802
BtgI C'CryG_G
Cuts at: 0 796 1194
Size: 796 398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 52 1194
Size: 52 1142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 980 1015 1194
Size: 980 35 179
Fragments arranged by size:
980 179 35
DraIII CAC_nnn'GTG
Cuts at: 0 531 1194
Size: 531 663
EaeI y'GGCC_r
Cuts at: 0 80 129 247 492 533 1194
Size: 80 49 118 245 41 661
Fragments arranged by size:
661 245 118 80 49 41
EagI C'GGCC_G
Cuts at: 0 247 492 1194
Size: 247 245 702
Fragments arranged by size:
702 247 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1012 1194
Size: 1012 182
EcoICRI GAG'CTC
Cuts at: 0 228 456 1194
Size: 228 228 738
Fragments arranged by size:
738 228 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 472 1194
Size: 282 190 722
Fragments arranged by size:
722 282 190
EcoNI CCTnn'n_nnAGG
Cuts at: 0 260 1194
Size: 260 934
EcoO109I rG'GnC_Cy
Cuts at: 0 242 415 1194
Size: 242 173 779
Fragments arranged by size:
779 242 173
HaeII r_GCGC'y
Cuts at: 0 35 269 612 1194
Size: 35 234 343 582
Fragments arranged by size:
582 343 234 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 1194
Size: 502 32 660
Fragments arranged by size:
660 502 32
KasI G'GCGC_C
Cuts at: 0 265 1194
Size: 265 929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1194
Size: 141 1053
MscI TGG'CCA
Cuts at: 0 535 1194
Size: 535 659
MslI CAynn'nnrTG
Cuts at: 0 442 757 1194
Size: 442 315 437
Fragments arranged by size:
442 437 315
MspA1I CmG'CkG
Cuts at: 0 60 177 405 618 1194
Size: 60 117 228 213 576
Fragments arranged by size:
576 228 213 117 60
NarI GG'CG_CC
Cuts at: 0 266 1194
Size: 266 928
NheI G'CTAG_C
Cuts at: 0 331 1194
Size: 331 863
NotI GC'GGCC_GC
Cuts at: 0 492 1194
Size: 492 702
NspI r_CATG'y
Cuts at: 0 441 978 1194
Size: 441 537 216
Fragments arranged by size:
537 441 216
PasI CC'CwG_GG
Cuts at: 0 506 1194
Size: 506 688
PflMI CCAn_nnn'nTGG
Cuts at: 0 618 1134 1194
Size: 618 516 60
Fragments arranged by size:
618 516 60
PshAI GACnn'nnGTC
Cuts at: 0 346 1194
Size: 346 848
PspOMI G'GGCC_C
Cuts at: 0 242 414 1194
Size: 242 172 780
Fragments arranged by size:
780 242 172
PstI C_TGCA'G
Cuts at: 0 182 608 1194
Size: 182 426 586
Fragments arranged by size:
586 426 182
PvuII CAG'CTG
Cuts at: 0 618 1194
Size: 618 576
RsrII CG'GwC_CG
Cuts at: 0 233 1194
Size: 233 961
SacI G_AGCT'C
Cuts at: 0 230 458 1194
Size: 230 228 736
Fragments arranged by size:
736 230 228
ScaI AGT'ACT
Cuts at: 0 577 1194
Size: 577 617
SexAI A'CCwGG_T
Cuts at: 0 1127 1194
Size: 1127 67
SfcI C'TryA_G
Cuts at: 0 55 178 604 1194
Size: 55 123 426 590
Fragments arranged by size:
590 426 123 55
SfoI GGC'GCC
Cuts at: 0 267 1194
Size: 267 927
SmaI CCC'GGG
Cuts at: 0 287 1194
Size: 287 907
SmlI C'TyrA_G
Cuts at: 0 571 1029 1194
Size: 571 458 165
Fragments arranged by size:
571 458 165
StyI C'CwwG_G
Cuts at: 0 99 1194
Size: 99 1095
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 611 1085 1194
Size: 336 275 474 109
Fragments arranged by size:
474 336 275 109
TatI w'GTAC_w
Cuts at: 0 575 1140 1194
Size: 575 565 54
Fragments arranged by size:
575 565 54
Tth111I GACn'n_nGTC
Cuts at: 0 514 1194
Size: 514 680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 1194
Size: 306 888
XmaI C'CCGG_G
Cuts at: 0 285 1194
Size: 285 909
Enzymes that do cut:
AarI AccI AlwNI ApaI AvaI BanI BanII BbeI
BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstZ17I Bsu36I BtgI BtgZI CspCI DraIII EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MmeI MscI MslI MspA1I NarI NheI NotI
NspI PasI PflMI PshAI PspOMI PstI PvuII RsrII
SacI ScaI SexAI SfcI SfoI SmaI SmlI StyI
TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BplI BsaI BsaAI BsaBI BsaXI
BsiWI BsmI BspHI BssHII BstAPI BstBI BstEII BstXI
BstYI BtsI ClaI DraI DrdI EarI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspXI PsrI PvuI SacII SalI SanDI SapI SbfI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XbaI XhoI XmnI ZraI