(Linear) (Six-base) MAPSORT of: KIEE1823.seq  Check: 8898  from: 1  to: 1008



KIEE1823.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0      38    1008 
   Size:         38     970
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317    1008 
   Size:        317     691
 
AflIII A'CryG_T

Cuts at:      0      95    1008 
   Size:         95     913
 
AgeI A'CCGG_T

Cuts at:      0     916    1008 
   Size:        916      92
 
ApoI r'AATT_y

Cuts at:      0     246     555    1008 
   Size:        246     309     453

  Fragments arranged by size: 

                 453     309     246
 
AvaI C'yCGr_G

Cuts at:      0     648    1008 
   Size:        648     360
 
BanI G'GyrC_C

Cuts at:      0     885    1008 
   Size:        885     123
 
BanII G_rGCy'C

Cuts at:      0      48    1008 
   Size:         48     960
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     125     867    1008 
   Size:        125     742     141

  Fragments arranged by size: 

                 742     141     125
 
BglII A'GATC_T

Cuts at:      0     852    1008 
   Size:        852     156
 
BmgBI CAC'GTC

Cuts at:      0     470    1008 
   Size:        470     538
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     782    1008 
   Size:        782     226
 
BsaWI w'CCGG_w

Cuts at:      0     916    1008 
   Size:        916      92
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     755     785    1008 
   Size:        755      30     223

  Fragments arranged by size: 

                 755     223      30
 
BseYI C'CCAG_C

Cuts at:      0     164    1008 
   Size:        164     844
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1008 
   Size:        499     509
 
BsiHKAI G_wGCw'C

Cuts at:      0      48    1008 
   Size:         48     960
 
BsmI GAATG_Cn'

Cuts at:      0     294    1008 
   Size:        294     714
 
Bsp1286I G_dGCh'C

Cuts at:      0      48    1008 
   Size:         48     960
 
BsrFI r'CCGG_y

Cuts at:      0     916    1008 
   Size:        916      92
 
BstYI r'GATC_y

Cuts at:      0     500     852    1008 
   Size:        500     352     156

  Fragments arranged by size: 

                 500     352     156
 
BstZ17I GTA'TAC

Cuts at:      0      39    1008 
   Size:         39     969
 
BtgI C'CryG_G

Cuts at:      0     546     600     938    1008 
   Size:        546      54     338      70

  Fragments arranged by size: 

                 546     338      70      54
 
BtsI GCAGTG_nn'

Cuts at:      0     491    1008 
   Size:        491     517
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     792     827    1008 
   Size:        792      35     181

  Fragments arranged by size: 

                 792     181      35
 
EarI CTCTTCn'nnn_

Cuts at:      0      32      50     843    1008 
   Size:         32      18     793     165

  Fragments arranged by size: 

                 793     165      32      18
 
EcoICRI GAG'CTC

Cuts at:      0      46    1008 
   Size:         46     962
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     782    1008 
   Size:        317     465     226

  Fragments arranged by size: 

                 465     317     226
 
EcoO109I rG'GnC_Cy

Cuts at:      0     161    1008 
   Size:        161     847
 
EcoRI G'AATT_C

Cuts at:      0     246    1008 
   Size:        246     762
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      79    1008 
   Size:         79     929
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114     837     869    1008 
   Size:         82      32     723      32     139

  Fragments arranged by size: 

                 723     139      82      32      32
 
HincII GTy'rAC

Cuts at:      0     922     969    1008 
   Size:        922      47      39

  Fragments arranged by size: 

                 922      47      39
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1000    1008 
   Size:       1000       8
 
MspA1I CmG'CkG

Cuts at:      0     397     662    1008 
   Size:        397     265     346

  Fragments arranged by size: 

                 397     346     265
 
NcoI C'CATG_G

Cuts at:      0     546     938    1008 
   Size:        546     392      70

  Fragments arranged by size: 

                 546     392      70
 
NspI r_CATG'y

Cuts at:      0      99    1008 
   Size:         99     909
 
PciI A'CATG_T

Cuts at:      0      95    1008 
   Size:         95     913
 
PpuMI rG'GwC_Cy

Cuts at:      0     161    1008 
   Size:        161     847
 
PstI C_TGCA'G

Cuts at:      0     598    1008 
   Size:        598     410
 
PvuII CAG'CTG

Cuts at:      0     397    1008 
   Size:        397     611
 
SacI G_AGCT'C

Cuts at:      0      48    1008 
   Size:         48     960
 
SanDI GG'GwC_CC

Cuts at:      0     161    1008 
   Size:        161     847
 
SapI GCTCTTCn'nnn_

Cuts at:      0      32    1008 
   Size:         32     976
 
SfcI C'TryA_G

Cuts at:      0     594     716     897    1008 
   Size:        594     122     181     111

  Fragments arranged by size: 

                 594     181     122     111
 
StyI C'CwwG_G

Cuts at:      0     546     938    1008 
   Size:        546     392      70

  Fragments arranged by size: 

                 546     392      70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     903    1008 
   Size:        903     105
 
XbaI T'CTAG_A

Cuts at:      0     102    1008 
   Size:        102     906
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     347     936    1008 
   Size:        347     589      72

  Fragments arranged by size: 

                 589     347      72
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AgeI     ApoI     AvaI     BanI    BanII
    BciVI    BglII    BmgBI     BpmI    BsaWI    BsaXI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BsrFI    BstYI  BstZ17I     BtgI     BtsI
    CspCI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I
   HincII     MmeI   MspA1I     NcoI     NspI     PciI    PpuMI     PstI
    PvuII     SacI    SanDI     SapI     SfcI     StyI    TaqII     XbaI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI
    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XhoI
     XmaI     XmnI     ZraI