Restriction map for
pF1KE1797
(Linear) (Six-base) MAPSORT of: KIEE1797.seq Check: 8696 from: 1 to: 2529
KIEE1797.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2251 2529
Size: 2251 278
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 466 1162 2362 2529
Size: 385 81 696 1200 167
Fragments arranged by size:
1200 696 385 167 81
AfeI AGC'GCT
Cuts at: 0 9 2529
Size: 9 2520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1747 2529
Size: 1747 782
ApaI G_GGCC'C
Cuts at: 0 1797 1815 2529
Size: 1797 18 714
Fragments arranged by size:
1797 714 18
ApaLI G'TGCA_C
Cuts at: 0 1615 2529
Size: 1615 914
ApoI r'AATT_y
Cuts at: 0 962 2321 2529
Size: 962 1359 208
Fragments arranged by size:
1359 962 208
AvaI C'yCGr_G
Cuts at: 0 643 1056 1125 1498 1826 2529
Size: 643 413 69 373 328 703
Fragments arranged by size:
703 643 413 373 328 69
BanI G'GyrC_C
Cuts at: 0 1248 1483 1976 2003 2529
Size: 1248 235 493 27 526
Fragments arranged by size:
1248 526 493 235 27
BanII G_rGCy'C
Cuts at: 0 1219 1226 1797 1815 2529
Size: 1219 7 571 18 714
Fragments arranged by size:
1219 714 571 18 7
BbeI G_GCGC'C
Cuts at: 0 1252 1487 2529
Size: 1252 235 1042
Fragments arranged by size:
1252 1042 235
BbsI GAAGACnn'nnnn_
Cuts at: 0 1304 2529
Size: 1304 1225
BbvCI CC'TCA_GC
Cuts at: 0 2114 2529
Size: 2114 415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2416 2450 2529
Size: 2416 34 79
Fragments arranged by size:
2416 79 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 309 363 802 2529
Size: 309 54 439 1727
Fragments arranged by size:
1727 439 309 54
BfrBI ATG'CAT
Cuts at: 0 1011 2529
Size: 1011 1518
BglI GCCn_nnn'nGGC
Cuts at: 0 198 1634 2529
Size: 198 1436 895
Fragments arranged by size:
1436 895 198
BglII A'GATC_T
Cuts at: 0 2459 2529
Size: 2459 70
BlpI GC'TnA_GC
Cuts at: 0 1115 2084 2383 2529
Size: 1115 969 299 146
Fragments arranged by size:
1115 969 299 146
Bme1580I G_kGCm'C
Cuts at: 0 1619 1797 1815 1911 2006 2028 2529
Size: 1619 178 18 96 95 22 501
Fragments arranged by size:
1619 501 178 96 95 22 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 2529
Size: 67 2462
BmtI G_CTAG'C
Cuts at: 0 2113 2529
Size: 2113 416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1140 2529
Size: 1140 1389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 982 1168 1192 1891 1940 2110 2213
Size: 316 666 186 24 699 49 170 103
Cuts at: 2213 2260 2529
Size: 47 269
Fragments arranged by size:
699 666 316 269 186 170 103 49
47 24
Bpu10I CC'TnA_GC
Cuts at: 0 2114 2529
Size: 2114 415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1324 2529
Size: 1324 1205
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 2529
Size: 88 2441
BsaHI Gr'CG_yC
Cuts at: 0 1249 1484 2529
Size: 1249 235 1045
Fragments arranged by size:
1249 1045 235
BsaWI w'CCGG_w
Cuts at: 0 210 1683 2330 2529
Size: 210 1473 647 199
Fragments arranged by size:
1473 647 210 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1252 1282 2529
Size: 1252 30 1247
Fragments arranged by size:
1252 1247 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 615 709 840 1189 1486 1600 2107 2470
Size: 615 94 131 349 297 114 507 363
Cuts at: 2470 2529
Size: 59
Fragments arranged by size:
615 507 363 349 297 131 114 94
59
BseYI C'CCAG_C
Cuts at: 0 37 1696 1807 2529
Size: 37 1659 111 722
Fragments arranged by size:
1659 722 111 37
BsiEI CG_ry'CG
Cuts at: 0 1472 2529
Size: 1472 1057
BsiHKAI G_wGCw'C
Cuts at: 0 1226 1619 2483 2529
Size: 1226 393 864 46
Fragments arranged by size:
1226 864 393 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1958 2467 2529
Size: 1958 509 62
Fragments arranged by size:
1958 509 62
Bsp1286I G_dGCh'C
Cuts at: 0 1219 1226 1619 1797 1815 1911 2006 2028
Size: 1219 7 393 178 18 96 95 22
Cuts at: 2028 2483 2529
Size: 455 46
Fragments arranged by size:
1219 455 393 178 96 95 46 22
18 7
BspEI T'CCGG_A
Cuts at: 0 210 1683 2529
Size: 210 1473 846
Fragments arranged by size:
1473 846 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2251 2529
Size: 2251 278
BsrBI CCG'CTC
Cuts at: 0 1394 1881 1949 2446 2520 2529
Size: 1394 487 68 497 74 9
Fragments arranged by size:
1394 497 487 74 68 9
BsrDI GCAATG_nn'
Cuts at: 0 348 1749 2529
Size: 348 1401 780
Fragments arranged by size:
1401 780 348
BsrFI r'CCGG_y
Cuts at: 0 1973 2529
Size: 1973 556
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1614 1866 2529
Size: 1614 252 663
Fragments arranged by size:
1614 663 252
BstEII G'GTnAC_C
Cuts at: 0 132 2529
Size: 132 2397
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2079 2289 2529
Size: 2079 210 240
Fragments arranged by size:
2079 240 210
BstYI r'GATC_y
Cuts at: 0 530 1190 2459 2529
Size: 530 660 1269 70
Fragments arranged by size:
1269 660 530 70
BtgI C'CryG_G
Cuts at: 0 64 737 770 788 1091 1466 2529
Size: 64 673 33 18 303 375 1063
Fragments arranged by size:
1063 673 375 303 64 33 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 997 2065 2441 2529
Size: 997 1068 376 88
Fragments arranged by size:
1068 997 376 88
BtsI GCAGTG_nn'
Cuts at: 0 957 1023 1617 1867 2529
Size: 957 66 594 250 662
Fragments arranged by size:
957 662 594 250 66
ClaI AT'CG_AT
Cuts at: 0 2525 2529
Size: 2525 4
DraI TTT'AAA
Cuts at: 0 2373 2529
Size: 2373 156
EaeI y'GGCC_r
Cuts at: 0 1032 1469 2150 2180 2529
Size: 1032 437 681 30 349
Fragments arranged by size:
1032 681 437 349 30
EagI C'GGCC_G
Cuts at: 0 1469 2529
Size: 1469 1060
EarI CTCTTCn'nnn_
Cuts at: 0 159 686 1199 2529
Size: 159 527 513 1330
Fragments arranged by size:
1330 527 513 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 183 1612 1817 2529
Size: 183 1429 205 712
Fragments arranged by size:
1429 712 205 183
EcoICRI GAG'CTC
Cuts at: 0 1224 2529
Size: 1224 1305
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 385 466 982 1162 1168 1192 1891
Size: 316 69 81 516 180 6 24 699
Cuts at: 1891 1940 2110 2213 2260 2362 2529
Size: 49 170 103 47 102 167
Fragments arranged by size:
699 516 316 180 170 167 103 102
81 69 49 47 24 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 822 1075 2103 2529
Size: 822 253 1028 426
Fragments arranged by size:
1028 822 426 253
EcoO109I rG'GnC_Cy
Cuts at: 0 815 1335 1793 1794 1811 1812 2043 2529
Size: 815 520 458 1 17 1 231 486
Fragments arranged by size:
815 520 486 458 231 17 1 1
EcoRI G'AATT_C
Cuts at: 0 962 2529
Size: 962 1567
EcoRV GAT'ATC
Cuts at: 0 905 2529
Size: 905 1624
HaeII r_GCGC'y
Cuts at: 0 11 890 1182 1252 1487 2529
Size: 11 879 292 70 235 1042
Fragments arranged by size:
1042 879 292 235 70 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 464 496 520 552 816 848 919 951
Size: 464 32 24 32 264 32 71 32
Cuts at: 951 1312 1344 2529
Size: 361 32 1185
Fragments arranged by size:
1185 464 361 264 71 32 32 32
32 32 24
HincII GTy'rAC
Cuts at: 0 801 2529
Size: 801 1728
KasI G'GCGC_C
Cuts at: 0 1248 1483 2529
Size: 1248 235 1046
Fragments arranged by size:
1248 1046 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 1133 2529
Size: 182 951 1396
Fragments arranged by size:
1396 951 182
MscI TGG'CCA
Cuts at: 0 1034 2152 2529
Size: 1034 1118 377
Fragments arranged by size:
1118 1034 377
MslI CAynn'nnrTG
Cuts at: 0 937 1016 1562 2529
Size: 937 79 546 967
Fragments arranged by size:
967 937 546 79
MspA1I CmG'CkG
Cuts at: 0 66 444 1114 1468 1611 2000 2088 2139
Size: 66 378 670 354 143 389 88 51
Cuts at: 2139 2529
Size: 390
Fragments arranged by size:
670 390 389 378 354 143 88 66
51
NaeI GCC'GGC
Cuts at: 0 1975 2529
Size: 1975 554
NarI GG'CG_CC
Cuts at: 0 1249 1484 2529
Size: 1249 235 1045
Fragments arranged by size:
1249 1045 235
NgoMIV G'CCGG_C
Cuts at: 0 1973 2529
Size: 1973 556
NheI G'CTAG_C
Cuts at: 0 2109 2529
Size: 2109 420
NotI GC'GGCC_GC
Cuts at: 0 1469 2529
Size: 1469 1060
NsiI A_TGCA'T
Cuts at: 0 1013 2529
Size: 1013 1516
NspI r_CATG'y
Cuts at: 0 1540 2529
Size: 1540 989
PasI CC'CwG_GG
Cuts at: 0 1630 2361 2529
Size: 1630 731 168
Fragments arranged by size:
1630 731 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 1170 2529
Size: 1170 1359
PfoI T'CCnGG_A
Cuts at: 0 29 293 2529
Size: 29 264 2236
Fragments arranged by size:
2236 264 29
PpuMI rG'GwC_Cy
Cuts at: 0 815 1335 2043 2529
Size: 815 520 708 486
Fragments arranged by size:
815 708 520 486
PspOMI G'GGCC_C
Cuts at: 0 1793 1811 2529
Size: 1793 18 718
Fragments arranged by size:
1793 718 18
PstI C_TGCA'G
Cuts at: 0 1187 1820 2529
Size: 1187 633 709
Fragments arranged by size:
1187 709 633
PvuII CAG'CTG
Cuts at: 0 444 1114 2088 2529
Size: 444 670 974 441
Fragments arranged by size:
974 670 444 441
SacI G_AGCT'C
Cuts at: 0 1226 2529
Size: 1226 1303
SacII CC_GC'GG
Cuts at: 0 67 1469 2529
Size: 67 1402 1060
Fragments arranged by size:
1402 1060 67
SfcI C'TryA_G
Cuts at: 0 1183 1377 1816 2529
Size: 1183 194 439 713
Fragments arranged by size:
1183 713 439 194
SfoI GGC'GCC
Cuts at: 0 1250 1485 2529
Size: 1250 235 1044
Fragments arranged by size:
1250 1044 235
SmaI CCC'GGG
Cuts at: 0 1828 2529
Size: 1828 701
SmlI C'TyrA_G
Cuts at: 0 1125 1339 2529
Size: 1125 214 1190
Fragments arranged by size:
1190 1125 214
StuI AGG'CCT
Cuts at: 0 1927 2098 2529
Size: 1927 171 431
Fragments arranged by size:
1927 431 171
StyI C'CwwG_G
Cuts at: 0 1076 1580 1676 1934 2529
Size: 1076 504 96 258 595
Fragments arranged by size:
1076 595 504 258 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 541 2053 2085 2529
Size: 541 1512 32 444
Fragments arranged by size:
1512 541 444 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1252 1284 2529
Size: 1252 32 1245
Fragments arranged by size:
1252 1245 32
Tth111I GACn'n_nGTC
Cuts at: 0 237 2529
Size: 237 2292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2220 2529
Size: 2220 309
XhoI C'TCGA_G
Cuts at: 0 1125 2529
Size: 1125 1404
XmaI C'CCGG_G
Cuts at: 0 1826 2529
Size: 1826 703
XmnI GAAnn'nnTTC
Cuts at: 0 2321 2529
Size: 2321 208
Enzymes that do cut:
AarI AcuI AfeI AlfI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NotI NsiI NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SacII SfcI SfoI SmaI SmlI
StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BclI BmgBI BsaAI BsaBI BsgI BsiWI BsmI
BspHI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I CspCI
DraIII DrdI FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NdeI NruI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI XbaI ZraI