(Linear) (Six-base) MAPSORT of: KIEE1716.seq  Check: 3506  from: 1  to: 660



KIEE1716.orf

 With 182 enzymes: SgfI * 

                        July 3, 2020 20:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     660 
   Size:        211     449
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     574     660 
   Size:        574      86
 
ApaI G_GGCC'C

Cuts at:      0     602     660 
   Size:        602      58
 
ApoI r'AATT_y

Cuts at:      0     300     660 
   Size:        300     360
 
AvaI C'yCGr_G

Cuts at:      0     595     660 
   Size:        595      65
 
BanII G_rGCy'C

Cuts at:      0     602     660 
   Size:        602      58
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     362     660 
   Size:        362     298
 
Bme1580I G_kGCm'C

Cuts at:      0     602     660 
   Size:        602      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     660 
   Size:        280     380
 
Bpu10I CC'TnA_GC

Cuts at:      0       4     112     660 
   Size:          4     108     548

  Fragments arranged by size: 

                 548     108       4
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     660 
   Size:        126     534
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     347     552     561     660 
   Size:        347     205       9      99

  Fragments arranged by size: 

                 347     205      99       9
 
BseYI C'CCAG_C

Cuts at:      0     218     660 
   Size:        218     442
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544     660 
   Size:        544     116
 
BsiWI C'GTAC_G

Cuts at:      0      92     660 
   Size:         92     568
 
Bsp1286I G_dGCh'C

Cuts at:      0     602     660 
   Size:        602      58
 
BsrDI GCAATG_nn'

Cuts at:      0     279     390     660 
   Size:        279     111     270

  Fragments arranged by size: 

                 279     270     111
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     103     660 
   Size:        103     557
 
EarI CTCTTCn'nnn_

Cuts at:      0     142     373     660 
   Size:        142     231     287

  Fragments arranged by size: 

                 287     231     142
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     280     660 
   Size:        211      69     380

  Fragments arranged by size: 

                 380     211      69
 
HincII GTy'rAC

Cuts at:      0     108     312     660 
   Size:        108     204     348

  Fragments arranged by size: 

                 348     204     108
 
HpaI GTT'AAC

Cuts at:      0     312     660 
   Size:        312     348
 
MslI CAynn'nnrTG

Cuts at:      0     577     660 
   Size:        577      83
 
PfoI T'CCnGG_A

Cuts at:      0     438     660 
   Size:        438     222
 
PspOMI G'GGCC_C

Cuts at:      0     598     660 
   Size:        598      62
 
SapI GCTCTTCn'nnn_

Cuts at:      0     373     660 
   Size:        373     287
 
SfcI C'TryA_G

Cuts at:      0     495     660 
   Size:        495     165
 
SmaI CCC'GGG

Cuts at:      0     597     660 
   Size:        597      63
 
SmlI C'TyrA_G

Cuts at:      0     141     660 
   Size:        141     519
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     450     522     660 
   Size:        450      72     138

  Fragments arranged by size: 

                 450     138      72
 
XmaI C'CCGG_G

Cuts at:      0     595     660 
   Size:        595      65
 

 Enzymes that do cut:

     AcuI     AlfI     ApaI     ApoI     AvaI    BanII    BciVI Bme1580I
     BpmI   Bpu10I    BpuEI    BseRI    BseYI     BsgI    BsiWI Bsp1286I
    BsrDI     DrdI     EarI  Eco57MI   HincII     HpaI     MslI     PfoI
   PspOMI     SapI     SfcI     SmaI     SmlI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI  BsiHKAI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI