Restriction map for
pF1KE1136
(Linear) (Six-base) MAPSORT of: KIEE1136.seq Check: 57 from: 1 to: 1071
KIEE1136.orf
With 182 enzymes: SgfI *
August 6, 2014 11:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 704 1071
Size: 704 367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 405 1071
Size: 92 313 666
Fragments arranged by size:
666 313 92
AlwNI CAG_nnn'CTG
Cuts at: 0 16 1071
Size: 16 1055
ApaI G_GGCC'C
Cuts at: 0 284 633 1020 1071
Size: 284 349 387 51
Fragments arranged by size:
387 349 284 51
ApoI r'AATT_y
Cuts at: 0 1000 1071
Size: 1000 71
AvaI C'yCGr_G
Cuts at: 0 787 1071
Size: 787 284
BamHI G'GATC_C
Cuts at: 0 305 1071
Size: 305 766
BanII G_rGCy'C
Cuts at: 0 284 633 1020 1036 1071
Size: 284 349 387 16 35
Fragments arranged by size:
387 349 284 35 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 1071
Size: 530 541
BbvCI CC'TCA_GC
Cuts at: 0 733 1071
Size: 733 338
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1071
Size: 32 1039
Bme1580I G_kGCm'C
Cuts at: 0 284 633 1020 1071
Size: 284 349 387 51
Fragments arranged by size:
387 349 284 51
Bpu10I CC'TnA_GC
Cuts at: 0 733 1071
Size: 733 338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 902 1071
Size: 887 15 169
Fragments arranged by size:
887 169 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 500 643 1071
Size: 500 143 428
Fragments arranged by size:
500 428 143
BseYI C'CCAG_C
Cuts at: 0 758 1071
Size: 758 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1071
Size: 187 884
BsiHKAI G_wGCw'C
Cuts at: 0 277 1071
Size: 277 794
Bsp1286I G_dGCh'C
Cuts at: 0 277 284 633 1020 1036 1071
Size: 277 7 349 387 16 35
Fragments arranged by size:
387 349 277 35 16 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 704 1071
Size: 704 367
BsrBI CCG'CTC
Cuts at: 0 508 1071
Size: 508 563
BsrDI GCAATG_nn'
Cuts at: 0 36 525 659 1071
Size: 36 489 134 412
Fragments arranged by size:
489 412 134 36
BssSI C'ACGA_G
Cuts at: 0 862 1071
Size: 862 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 672 1071
Size: 672 399
BstYI r'GATC_y
Cuts at: 0 305 1071
Size: 305 766
BtgI C'CryG_G
Cuts at: 0 413 821 1012 1071
Size: 413 408 191 59
Fragments arranged by size:
413 408 191 59
EaeI y'GGCC_r
Cuts at: 0 101 410 1027 1071
Size: 101 309 617 44
Fragments arranged by size:
617 309 101 44
EarI CTCTTCn'nnn_
Cuts at: 0 214 559 613 865 923 1071
Size: 214 345 54 252 58 148
Fragments arranged by size:
345 252 214 148 58 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 729 1071
Size: 729 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 405 1071
Size: 92 313 666
Fragments arranged by size:
666 313 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 430 1071
Size: 430 641
EcoO109I rG'GnC_Cy
Cuts at: 0 280 629 1071
Size: 280 349 442
Fragments arranged by size:
442 349 280
EcoRI G'AATT_C
Cuts at: 0 1000 1071
Size: 1000 71
HaeII r_GCGC'y
Cuts at: 0 568 1071
Size: 568 503
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 390 422 1071
Size: 390 32 649
Fragments arranged by size:
649 390 32
HindIII A'AGCT_T
Cuts at: 0 74 1071
Size: 74 997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 1071
Size: 200 871
MscI TGG'CCA
Cuts at: 0 103 1071
Size: 103 968
MslI CAynn'nnrTG
Cuts at: 0 670 1071
Size: 670 401
MspA1I CmG'CkG
Cuts at: 0 975 1071
Size: 975 96
NcoI C'CATG_G
Cuts at: 0 821 1071
Size: 821 250
PflMI CCAn_nnn'nTGG
Cuts at: 0 758 1071
Size: 758 313
PfoI T'CCnGG_A
Cuts at: 0 431 1071
Size: 431 640
PshAI GACnn'nnGTC
Cuts at: 0 481 1071
Size: 481 590
PspOMI G'GGCC_C
Cuts at: 0 280 629 1016 1071
Size: 280 349 387 55
Fragments arranged by size:
387 349 280 55
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 880 912 1071
Size: 880 32 159
Fragments arranged by size:
880 159 32
PstI C_TGCA'G
Cuts at: 0 980 1071
Size: 980 91
PvuII CAG'CTG
Cuts at: 0 975 1071
Size: 975 96
SapI GCTCTTCn'nnn_
Cuts at: 0 865 1071
Size: 865 206
ScaI AGT'ACT
Cuts at: 0 49 1071
Size: 49 1022
SfcI C'TryA_G
Cuts at: 0 51 976 1071
Size: 51 925 95
Fragments arranged by size:
925 95 51
SmlI C'TyrA_G
Cuts at: 0 902 917 1071
Size: 902 15 154
Fragments arranged by size:
902 154 15
StyI C'CwwG_G
Cuts at: 0 743 821 1071
Size: 743 78 250
Fragments arranged by size:
743 250 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 660 750 1071
Size: 208 452 90 321
Fragments arranged by size:
452 321 208 90
TatI w'GTAC_w
Cuts at: 0 47 1071
Size: 47 1024
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 1071
Size: 278 32 761
Fragments arranged by size:
761 278 32
Tth111I GACn'n_nGTC
Cuts at: 0 529 1071
Size: 529 542
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BamHI BanII
BbsI BbvCI BglI Bme1580I Bpu10I BpuEI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssSI BstXI
BstYI BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HindIII MmeI MscI MslI MspA1I
NcoI PflMI PfoI PshAI PspOMI PsrI PstI PvuII
SapI ScaI SfcI SmlI StyI TaqII TatI TstI
Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PsiI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XcmI XhoI
XmaI XmnI ZraI