(Linear) (Six-base) MAPSORT of: KIEE1099.seq  Check: 9285  from: 1  to: 960



KIEE1099.orf

 With 182 enzymes: SgfI * 

                        November 19, 2009 09:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     153     960 
   Size:        153     807
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     960 
   Size:        410     550
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     260     960 
   Size:        260     700
 
AlwNI CAG_nnn'CTG

Cuts at:      0     730     960 
   Size:        730     230
 
ApoI r'AATT_y

Cuts at:      0     885     960 
   Size:        885      75
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     733     960 
   Size:        733     227
 
BbvCI CC'TCA_GC

Cuts at:      0       5     960 
   Size:          5     955
 
BclI T'GATC_A

Cuts at:      0     519     960 
   Size:        519     441
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     960 
   Size:        557     403
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     960 
   Size:          5     955
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     960 
   Size:        286     674
 
BsaHI Gr'CG_yC

Cuts at:      0     738     960 
   Size:        738     222
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     910     960 
   Size:         88     822      50

  Fragments arranged by size: 

                 822      88      50
 
BsmI GAATG_Cn'

Cuts at:      0     222     527     960 
   Size:        222     305     433

  Fragments arranged by size: 

                 433     305     222
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     153     960 
   Size:        153     807
 
BsrDI GCAATG_nn'

Cuts at:      0      59     960 
   Size:         59     901
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     168     960 
   Size:        168     792
 
Bsu36I CC'TnA_GG

Cuts at:      0     893     960 
   Size:        893      67
 
DraI TTT'AAA

Cuts at:      0     834     960 
   Size:        834     126
 
EarI CTCTTCn'nnn_

Cuts at:      0     386     940     960 
   Size:        386     554      20

  Fragments arranged by size: 

                 554     386      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     557     960 
   Size:        410     147     403

  Fragments arranged by size: 

                 410     403     147
 
EcoRV GAT'ATC

Cuts at:      0     471     960 
   Size:        471     489
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     569     960 
   Size:        569     391
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     886     918     960 
   Size:        886      32      42

  Fragments arranged by size: 

                 886      42      32
 
MslI CAynn'nnrTG

Cuts at:      0     865     960 
   Size:        865      95
 
MspA1I CmG'CkG

Cuts at:      0     730     960 
   Size:        730     230
 
NspI r_CATG'y

Cuts at:      0     434     960 
   Size:        434     526
 
PvuII CAG'CTG

Cuts at:      0     730     960 
   Size:        730     230
 
ScaI AGT'ACT

Cuts at:      0     552     960 
   Size:        552     408
 
SfcI C'TryA_G

Cuts at:      0     663     960 
   Size:        663     297
 
SmlI C'TyrA_G

Cuts at:      0     265     960 
   Size:        265     695
 
SpeI A'CTAG_T

Cuts at:      0     864     960 
   Size:        864      96
 
SspI AAT'ATT

Cuts at:      0     107     960 
   Size:        107     853
 
StyI C'CwwG_G

Cuts at:      0     223     960 
   Size:        223     737
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     303     557     661     960 
   Size:        303     254     104     299

  Fragments arranged by size: 

                 303     299     254     104
 
TatI w'GTAC_w

Cuts at:      0     550     960 
   Size:        550     410
 
XmnI GAAnn'nnTTC

Cuts at:      0     299     960 
   Size:        299     661
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApoI     BbsI    BbvCI     BclI
     BpmI   Bpu10I    BpuEI    BsaHI    BseRI     BsmI    BspMI    BsrDI
   BstAPI   Bsu36I     DraI     EarI  Eco57MI    EcoRV     FalI    Hin4I
     MslI   MspA1I     NspI    PvuII     ScaI     SfcI     SmlI     SpeI
     SspI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI