Restriction map for
pF1KE1095
(Linear) (Six-base) MAPSORT of: KIEE1095.seq Check: 4203 from: 1 to: 1080
KIEE1095.orf
With 182 enzymes: SgfI *
October 26, 2009 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 129 1080
Size: 129 951
Acc65I G'GTAC_C
Cuts at: 0 640 1080
Size: 640 440
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 717 1080
Size: 376 341 363
Fragments arranged by size:
376 363 341
AflIII A'CryG_T
Cuts at: 0 1046 1080
Size: 1046 34
AleI CACnn'nnGTG
Cuts at: 0 494 616 1080
Size: 494 122 464
Fragments arranged by size:
494 464 122
AlwNI CAG_nnn'CTG
Cuts at: 0 613 1080
Size: 613 467
AvaI C'yCGr_G
Cuts at: 0 254 419 483 1080
Size: 254 165 64 597
Fragments arranged by size:
597 254 165 64
BanI G'GyrC_C
Cuts at: 0 538 640 1080
Size: 538 102 440
Fragments arranged by size:
538 440 102
BanII G_rGCy'C
Cuts at: 0 107 440 1080
Size: 107 333 640
Fragments arranged by size:
640 333 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 999 1080
Size: 999 81
BbvCI CC'TCA_GC
Cuts at: 0 624 1080
Size: 624 456
BciVI GTATCCnnnnn_n'
Cuts at: 0 795 1080
Size: 795 285
BglII A'GATC_T
Cuts at: 0 227 1080
Size: 227 853
BmgBI CAC'GTC
Cuts at: 0 1047 1080
Size: 1047 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 481 1080
Size: 310 171 599
Fragments arranged by size:
599 310 171
Bpu10I CC'TnA_GC
Cuts at: 0 624 1080
Size: 624 456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1080
Size: 781 299
BsaAI yAC'GTr
Cuts at: 0 870 1080
Size: 870 210
BsaBI GATnn'nnATC
Cuts at: 0 346 1080
Size: 346 734
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 805 835 1080
Size: 730 30 45 30 245
Fragments arranged by size:
730 245 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 35 735 1080
Size: 35 700 345
Fragments arranged by size:
700 345 35
BseYI C'CCAG_C
Cuts at: 0 454 1080
Size: 454 626
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1080
Size: 154 926
BsiEI CG_ry'CG
Cuts at: 0 939 1080
Size: 939 141
BsiHKAI G_wGCw'C
Cuts at: 0 529 1080
Size: 529 551
Bsp1286I G_dGCh'C
Cuts at: 0 107 440 529 1080
Size: 107 333 89 551
Fragments arranged by size:
551 333 107 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 1080
Size: 129 951
BsrBI CCG'CTC
Cuts at: 0 176 821 1080
Size: 176 645 259
Fragments arranged by size:
645 259 176
BsrDI GCAATG_nn'
Cuts at: 0 968 1080
Size: 968 112
BssSI C'ACGA_G
Cuts at: 0 133 1080
Size: 133 947
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 1080
Size: 186 894
BstYI r'GATC_y
Cuts at: 0 227 1080
Size: 227 853
BtgI C'CryG_G
Cuts at: 0 71 1080
Size: 71 1009
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 376 481 717 1080
Size: 310 66 105 236 363
Fragments arranged by size:
363 310 236 105 66
EcoO109I rG'GnC_Cy
Cuts at: 0 850 989 1080
Size: 850 139 91
Fragments arranged by size:
850 139 91
FspI TGC'GCA
Cuts at: 0 88 325 1080
Size: 88 237 755
Fragments arranged by size:
755 237 88
FspAI rTGC'GCAy
Cuts at: 0 88 325 1080
Size: 88 237 755
Fragments arranged by size:
755 237 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 652 684 1080
Size: 652 32 396
Fragments arranged by size:
652 396 32
HincII GTy'rAC
Cuts at: 0 960 1080
Size: 960 120
KpnI G_GTAC'C
Cuts at: 0 644 1080
Size: 644 436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1080
Size: 203 877
MslI CAynn'nnrTG
Cuts at: 0 370 494 575 616 1001 1080
Size: 370 124 81 41 385 79
Fragments arranged by size:
385 370 124 81 79 41
NcoI C'CATG_G
Cuts at: 0 71 1080
Size: 71 1009
NspI r_CATG'y
Cuts at: 0 330 1080
Size: 330 750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 946 978 1080
Size: 946 32 102
Fragments arranged by size:
946 102 32
PpuMI rG'GwC_Cy
Cuts at: 0 989 1080
Size: 989 91
SfcI C'TryA_G
Cuts at: 0 614 1080
Size: 614 466
SmaI CCC'GGG
Cuts at: 0 256 421 485 1080
Size: 256 165 64 595
Fragments arranged by size:
595 256 165 64
SmlI C'TyrA_G
Cuts at: 0 796 1080
Size: 796 284
SphI G_CATG'C
Cuts at: 0 330 1080
Size: 330 750
StyI C'CwwG_G
Cuts at: 0 71 191 272 431 671 1080
Size: 71 120 81 159 240 409
Fragments arranged by size:
409 240 159 120 81 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 1080
Size: 925 155
TatI w'GTAC_w
Cuts at: 0 374 1080
Size: 374 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 730 762 865 897 1080
Size: 730 32 103 32 183
Fragments arranged by size:
730 183 103 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 678 1080
Size: 279 399 402
Fragments arranged by size:
402 399 279
XmaI C'CCGG_G
Cuts at: 0 254 419 483 1080
Size: 254 165 64 597
Fragments arranged by size:
597 254 165 64
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlwNI AvaI BanI
BanII BbsI BbvCI BciVI BglII BmgBI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssSI BstXI BstYI
BtgI Eco57MI EcoO109I FspI FspAI Hin4I HincII KpnI
MmeI MslI NcoI NspI PpiI PpuMI SfcI SmaI
SmlI SphI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BglI BlpI
Bme1580I BmrI BmtI BplI BsaI BsaHI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI HaeII HindIII HpaI
KasI MfeI MluI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmnI ZraI