Restriction map for
pF1KE1063
(Linear) (Six-base) MAPSORT of: KIEE1063.seq Check: 7568 from: 1 to: 1743
KIEE1063.orf
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 226 1743
Size: 226 1517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 364 765 1164 1500 1584 1743
Size: 156 208 401 399 336 84 159
Fragments arranged by size:
401 399 336 208 159 156 84
AflIII A'CryG_T
Cuts at: 0 35 1743
Size: 35 1708
AleI CACnn'nnGTG
Cuts at: 0 1058 1743
Size: 1058 685
AlwNI CAG_nnn'CTG
Cuts at: 0 72 1670 1743
Size: 72 1598 73
Fragments arranged by size:
1598 73 72
ApoI r'AATT_y
Cuts at: 0 304 1049 1214 1466 1550 1743
Size: 304 745 165 252 84 193
Fragments arranged by size:
745 304 252 193 165 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 1264 1743
Size: 1264 479
BfrBI ATG'CAT
Cuts at: 0 1386 1743
Size: 1386 357
BmgBI CAC'GTC
Cuts at: 0 36 1743
Size: 36 1707
BmrI ACTGGGnnnn_n'
Cuts at: 0 665 1103 1743
Size: 665 438 640
Fragments arranged by size:
665 640 438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1743
Size: 76 1667
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1743
Size: 133 1610
BsaI GGTCTCn'nnnn_
Cuts at: 0 1476 1743
Size: 1476 267
BsaWI w'CCGG_w
Cuts at: 0 739 1743
Size: 739 1004
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 410 440 1743
Size: 410 30 1303
Fragments arranged by size:
1303 410 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 1743
Size: 73 1670
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1095 1743
Size: 254 841 648
Fragments arranged by size:
841 648 254
BsmI GAATG_Cn'
Cuts at: 0 322 763 847 1330 1388 1498 1666 1681
Size: 322 441 84 483 58 110 168 15
Cuts at: 1681 1743
Size: 62
Fragments arranged by size:
483 441 322 168 110 84 62 58
15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 226 579 1743
Size: 226 353 1164
Fragments arranged by size:
1164 353 226
BsrGI T'GTAC_A
Cuts at: 0 720 1371 1743
Size: 720 651 372
Fragments arranged by size:
720 651 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 257 1743
Size: 257 1486
BtgI C'CryG_G
Cuts at: 0 137 1743
Size: 137 1606
BtsI GCAGTG_nn'
Cuts at: 0 852 1083 1743
Size: 852 231 660
Fragments arranged by size:
852 660 231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1401 1436 1743
Size: 1401 35 307
Fragments arranged by size:
1401 307 35
DraI TTT'AAA
Cuts at: 0 470 1743
Size: 470 1273
DraIII CAC_nnn'GTG
Cuts at: 0 132 1743
Size: 132 1611
EaeI y'GGCC_r
Cuts at: 0 2 1743
Size: 2 1741
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 156 364 765 1164 1500 1584 1743
Size: 76 80 208 401 399 336 84 159
Fragments arranged by size:
401 399 336 208 159 84 80 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1518 1743
Size: 1518 225
EcoO109I rG'GnC_Cy
Cuts at: 0 215 1743
Size: 215 1528
EcoRI G'AATT_C
Cuts at: 0 1049 1214 1466 1550 1743
Size: 1049 165 252 84 193
Fragments arranged by size:
1049 252 193 165 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 876 908 945 977 1443 1475 1743
Size: 876 32 37 32 466 32 268
Fragments arranged by size:
876 466 268 37 32 32 32
HincII GTy'rAC
Cuts at: 0 267 794 878 1361 1529 1697 1743
Size: 267 527 84 483 168 168 46
Fragments arranged by size:
527 483 267 168 168 84 46
HindIII A'AGCT_T
Cuts at: 0 1176 1743
Size: 1176 567
MscI TGG'CCA
Cuts at: 0 4 1743
Size: 4 1739
MslI CAynn'nnrTG
Cuts at: 0 924 1058 1743
Size: 924 134 685
Fragments arranged by size:
924 685 134
NsiI A_TGCA'T
Cuts at: 0 1388 1743
Size: 1388 355
PfoI T'CCnGG_A
Cuts at: 0 196 1743
Size: 196 1547
PpuMI rG'GwC_Cy
Cuts at: 0 215 1743
Size: 215 1528
PshAI GACnn'nnGTC
Cuts at: 0 935 1743
Size: 935 808
PsiI TTA'TAA
Cuts at: 0 551 1007 1743
Size: 551 456 736
Fragments arranged by size:
736 551 456
PstI C_TGCA'G
Cuts at: 0 576 1743
Size: 576 1167
SanDI GG'GwC_CC
Cuts at: 0 215 1743
Size: 215 1528
SexAI A'CCwGG_T
Cuts at: 0 128 183 1743
Size: 128 55 1560
Fragments arranged by size:
1560 128 55
SfcI C'TryA_G
Cuts at: 0 96 120 572 1743
Size: 96 24 452 1171
Fragments arranged by size:
1171 452 96 24
SmlI C'TyrA_G
Cuts at: 0 112 1743
Size: 112 1631
SspI AAT'ATT
Cuts at: 0 436 1743
Size: 436 1307
StuI AGG'CCT
Cuts at: 0 1597 1743
Size: 1597 146
TatI w'GTAC_w
Cuts at: 0 676 720 1021 1371 1743
Size: 676 44 301 350 372
Fragments arranged by size:
676 372 350 301 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 1743
Size: 118 32 1593
Fragments arranged by size:
1593 118 32
XmnI GAAnn'nnTTC
Cuts at: 0 1177 1261 1597 1681 1743
Size: 1177 84 336 84 62
Fragments arranged by size:
1177 336 84 84 62
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApoI BbsI BfrBI
BmgBI BmrI BpmI BpuEI BsaI BsaWI BsaXI BseRI
BsgI BsmI BspMI BsrGI BstXI BtgI BtsI CspCI
DraI DraIII EaeI Eco57MI EcoNI EcoO109I EcoRI Hin4I
HincII HindIII MscI MslI NsiI PfoI PpuMI PshAI
PsiI PstI SanDI SexAI SfcI SmlI SspI StuI
TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BseYI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI DrdI EagI EarI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI