Restriction map for
pF1KE1041
(Linear) (Six-base) MAPSORT of: KIEE1041.seq Check: 1749 from: 1 to: 1050
KIEE1041.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 91 302 452 814 947 1050
Size: 73 18 211 150 362 133 103
Fragments arranged by size:
362 211 150 133 103 73 18
AhdI GACnn_n'nnGTC
Cuts at: 0 750 1050
Size: 750 300
AleI CACnn'nnGTG
Cuts at: 0 815 1050
Size: 815 235
AlwNI CAG_nnn'CTG
Cuts at: 0 355 1050
Size: 355 695
ApaI G_GGCC'C
Cuts at: 0 313 1050
Size: 313 737
ApoI r'AATT_y
Cuts at: 0 760 1050
Size: 760 290
BanII G_rGCy'C
Cuts at: 0 313 1050
Size: 313 737
BbsI GAAGACnn'nnnn_
Cuts at: 0 862 1050
Size: 862 188
Bme1580I G_kGCm'C
Cuts at: 0 79 313 1050
Size: 79 234 737
Fragments arranged by size:
737 234 79
BmgBI CAC'GTC
Cuts at: 0 601 1050
Size: 601 449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 932 971 1050
Size: 458 474 39 79
Fragments arranged by size:
474 458 79 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 1050
Size: 495 555
BsaAI yAC'GTr
Cuts at: 0 728 1050
Size: 728 322
BseYI C'CCAG_C
Cuts at: 0 605 984 1050
Size: 605 379 66
Fragments arranged by size:
605 379 66
BsmI GAATG_Cn'
Cuts at: 0 508 1050
Size: 508 542
Bsp1286I G_dGCh'C
Cuts at: 0 79 313 1050
Size: 79 234 737
Fragments arranged by size:
737 234 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 1050
Size: 189 861
BsrDI GCAATG_nn'
Cuts at: 0 185 600 795 1050
Size: 185 415 195 255
Fragments arranged by size:
415 255 195 185
BsrGI T'GTAC_A
Cuts at: 0 549 1050
Size: 549 501
BstAPI GCAn_nnn'nTGC
Cuts at: 0 404 1050
Size: 404 646
BstBI TT'CG_AA
Cuts at: 0 585 1050
Size: 585 465
BstEII G'GTnAC_C
Cuts at: 0 1039 1050
Size: 1039 11
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 582 617 1050
Size: 582 35 433
Fragments arranged by size:
582 433 35
EaeI y'GGCC_r
Cuts at: 0 31 1050
Size: 31 1019
EarI CTCTTCn'nnn_
Cuts at: 0 911 1050
Size: 911 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 91 302 452 458 814 932 947
Size: 73 18 211 150 6 356 118 15
Cuts at: 947 971 1050
Size: 24 79
Fragments arranged by size:
356 211 150 118 79 73 24 18
15 6
EcoO109I rG'GnC_Cy
Cuts at: 0 309 1050
Size: 309 741
EcoRI G'AATT_C
Cuts at: 0 760 1050
Size: 760 290
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 656 688 736 768 1050
Size: 656 32 48 32 282
Fragments arranged by size:
656 282 48 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 1050
Size: 642 408
MscI TGG'CCA
Cuts at: 0 33 1050
Size: 33 1017
MslI CAynn'nnrTG
Cuts at: 0 815 1050
Size: 815 235
MspA1I CmG'CkG
Cuts at: 0 815 1050
Size: 815 235
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 1050
Size: 355 695
PfoI T'CCnGG_A
Cuts at: 0 354 1050
Size: 354 696
PsiI TTA'TAA
Cuts at: 0 455 1050
Size: 455 595
PspOMI G'GGCC_C
Cuts at: 0 309 1050
Size: 309 741
PstI C_TGCA'G
Cuts at: 0 233 1050
Size: 233 817
PvuII CAG'CTG
Cuts at: 0 815 1050
Size: 815 235
SapI GCTCTTCn'nnn_
Cuts at: 0 911 1050
Size: 911 139
SexAI A'CCwGG_T
Cuts at: 0 483 1050
Size: 483 567
SfcI C'TryA_G
Cuts at: 0 229 1050
Size: 229 821
SmlI C'TyrA_G
Cuts at: 0 510 1050
Size: 510 540
SspI AAT'ATT
Cuts at: 0 7 1050
Size: 7 1043
TatI w'GTAC_w
Cuts at: 0 194 549 1050
Size: 194 355 501
Fragments arranged by size:
501 355 194
Enzymes that do cut:
AcuI AhdI AleI AlwNI ApaI ApoI BanII BbsI
Bme1580I BmgBI BpmI BpuEI BsaAI BseYI BsmI Bsp1286I
BspMI BsrDI BsrGI BstAPI BstBI BstEII CspCI EaeI
EarI Eco57MI EcoO109I EcoRI Hin4I MmeI MscI MslI
MspA1I PflMI PfoI PsiI PspOMI PstI PvuII SapI
SexAI SfcI SmlI SspI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI