(Linear) (Six-base) MAPSORT of: KIEE1004.seq  Check: 7360  from: 1  to: 486



KIEE1004.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:28  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      83     115     486 
   Size:         83      32     371

  Fragments arranged by size: 

                 371      83      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      68     133     486 
   Size:         68      65     353

  Fragments arranged by size: 

                 353      68      65
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      78     486 
   Size:         78     408
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     486 
   Size:         50     436
 
Bpu10I CC'TnA_GC

Cuts at:      0      23     486 
   Size:         23     463
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     447     486 
   Size:        447      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      26     486 
   Size:         26     460
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     304     486 
   Size:        304     182
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     486 
   Size:        158     328
 
BsiHKAI G_wGCw'C

Cuts at:      0     314     344     486 
   Size:        314      30     142

  Fragments arranged by size: 

                 314     142      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     314     344     486 
   Size:        314      30     142

  Fragments arranged by size: 

                 314     142      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     309     415     486 
   Size:        309     106      71

  Fragments arranged by size: 

                 309     106      71
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     101     387     486 
   Size:        101     286      99

  Fragments arranged by size: 

                 286     101      99
 
BtsI GCAGTG_nn'

Cuts at:      0     384     486 
   Size:        384     102
 
DraIII CAC_nnn'GTG

Cuts at:      0     348     486 
   Size:        348     138
 
EaeI y'GGCC_r

Cuts at:      0     203     486 
   Size:        203     283
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     144     301     486 
   Size:        144     157     185

  Fragments arranged by size: 

                 185     157     144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     486 
   Size:         50     436
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     235     267     383     415     486 
   Size:        235      32     116      32      71

  Fragments arranged by size: 

                 235     116      71      32      32
 
HincII GTy'rAC

Cuts at:      0     228     486 
   Size:        228     258
 
MspA1I CmG'CkG

Cuts at:      0     258     282     486 
   Size:        258      24     204

  Fragments arranged by size: 

                 258     204      24
 
PstI C_TGCA'G

Cuts at:      0     268     486 
   Size:        268     218
 
ScaI AGT'ACT

Cuts at:      0     250     486 
   Size:        250     236
 
SexAI A'CCwGG_T

Cuts at:      0     221     383     486 
   Size:        221     162     103

  Fragments arranged by size: 

                 221     162     103
 
SfcI C'TryA_G

Cuts at:      0      60     264     486 
   Size:         60     204     222

  Fragments arranged by size: 

                 222     204      60
 
SmlI C'TyrA_G

Cuts at:      0     462     486 
   Size:        462      24
 
TatI w'GTAC_w

Cuts at:      0     248     486 
   Size:        248     238
 
Tth111I GACn'n_nGTC

Cuts at:      0     385     486 
   Size:        385     101
 

 Enzymes that do cut:

     AloI    AlwNI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BseRI
     BsgI  BsiHKAI Bsp1286I   BstAPI    BtgZI     BtsI   DraIII     EaeI
     EciI  Eco57MI    Hin4I   HincII   MspA1I     PstI     ScaI    SexAI
     SfcI     SmlI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI     DrdI     EagI     EarI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI