Restriction map for
pF1KE0970
(Linear) (Six-base) MAPSORT of: KIEE0970.seq Check: 3174 from: 1 to: 1569
KIEE0970.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 0 851 1569
Size: 851 718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 364 1413 1569
Size: 190 174 1049 156
Fragments arranged by size:
1049 190 174 156
AfeI AGC'GCT
Cuts at: 0 1391 1569
Size: 1391 178
AflIII A'CryG_T
Cuts at: 0 1367 1569
Size: 1367 202
AlwNI CAG_nnn'CTG
Cuts at: 0 297 1056 1101 1284 1569
Size: 297 759 45 183 285
Fragments arranged by size:
759 297 285 183 45
ApaLI G'TGCA_C
Cuts at: 0 220 913 1569
Size: 220 693 656
Fragments arranged by size:
693 656 220
ApoI r'AATT_y
Cuts at: 0 1021 1569
Size: 1021 548
BanI G'GyrC_C
Cuts at: 0 271 1336 1569
Size: 271 1065 233
Fragments arranged by size:
1065 271 233
BanII G_rGCy'C
Cuts at: 0 254 306 552 559 1493 1569
Size: 254 52 246 7 934 76
Fragments arranged by size:
934 254 246 76 52 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1303 1569
Size: 1303 266
BglI GCCn_nnn'nGGC
Cuts at: 0 10 475 872 1569
Size: 10 465 397 697
Fragments arranged by size:
697 465 397 10
BglII A'GATC_T
Cuts at: 0 211 408 857 1442 1569
Size: 211 197 449 585 127
Fragments arranged by size:
585 449 211 197 127
BlpI GC'TnA_GC
Cuts at: 0 1397 1569
Size: 1397 172
Bme1580I G_kGCm'C
Cuts at: 0 224 274 917 1339 1569
Size: 224 50 643 422 230
Fragments arranged by size:
643 422 230 224 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 765 1111 1569
Size: 765 346 458
Fragments arranged by size:
765 458 346
BmtI G_CTAG'C
Cuts at: 0 1396 1569
Size: 1396 173
Bpu10I CC'TnA_GC
Cuts at: 0 718 1569
Size: 718 851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 1569
Size: 1 1568
BsaAI yAC'GTr
Cuts at: 0 1269 1569
Size: 1269 300
BsaHI Gr'CG_yC
Cuts at: 0 240 848 1569
Size: 240 608 721
Fragments arranged by size:
721 608 240
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 340 1249 1569
Size: 208 132 909 320
Fragments arranged by size:
909 320 208 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1569
Size: 337 1232
BsiEI CG_ry'CG
Cuts at: 0 1153 1569
Size: 1153 416
BsiHKAI G_wGCw'C
Cuts at: 0 224 254 559 587 917 1403 1493 1569
Size: 224 30 305 28 330 486 90 76
Fragments arranged by size:
486 330 305 224 90 76 30 28
BsmI GAATG_Cn'
Cuts at: 0 699 1569
Size: 699 870
BsmBI CGTCTCn'nnnn_
Cuts at: 0 247 1569
Size: 247 1322
Bsp1286I G_dGCh'C
Cuts at: 0 224 254 274 306 552 559 587 917
Size: 224 30 20 32 246 7 28 330
Cuts at: 917 1339 1403 1493 1569
Size: 422 64 90 76
Fragments arranged by size:
422 330 246 224 90 76 64 32
30 28 20 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 665 798 1316 1569
Size: 665 133 518 253
Fragments arranged by size:
665 518 253 133
BsrBI CCG'CTC
Cuts at: 0 774 1569
Size: 774 795
BsrDI GCAATG_nn'
Cuts at: 0 942 1569
Size: 942 627
BstYI r'GATC_y
Cuts at: 0 211 386 408 422 857 1113 1442 1569
Size: 211 175 22 14 435 256 329 127
Fragments arranged by size:
435 329 256 211 175 127 22 14
BtgI C'CryG_G
Cuts at: 0 767 993 1569
Size: 767 226 576
Fragments arranged by size:
767 576 226
BtsI GCAGTG_nn'
Cuts at: 0 500 915 1193 1569
Size: 500 415 278 376
Fragments arranged by size:
500 415 376 278
EaeI y'GGCC_r
Cuts at: 0 2 518 1229 1569
Size: 2 516 711 340
Fragments arranged by size:
711 516 340 2
EarI CTCTTCn'nnn_
Cuts at: 0 166 1069 1569
Size: 166 903 500
Fragments arranged by size:
903 500 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 379 525 988 1015 1569
Size: 379 146 463 27 554
Fragments arranged by size:
554 463 379 146 27
EcoICRI GAG'CTC
Cuts at: 0 252 557 1491 1569
Size: 252 305 934 78
Fragments arranged by size:
934 305 252 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 364 1413 1569
Size: 190 174 1049 156
Fragments arranged by size:
1049 190 174 156
EcoNI CCTnn'n_nnAGG
Cuts at: 0 478 577 1569
Size: 478 99 992
Fragments arranged by size:
992 478 99
EcoO109I rG'GnC_Cy
Cuts at: 0 741 759 777 1436 1569
Size: 741 18 18 659 133
Fragments arranged by size:
741 659 133 18 18
EcoRI G'AATT_C
Cuts at: 0 1021 1569
Size: 1021 548
HaeII r_GCGC'y
Cuts at: 0 107 1352 1393 1569
Size: 107 1245 41 176
Fragments arranged by size:
1245 176 107 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 1239 1271 1569
Size: 691 32 516 32 298
Fragments arranged by size:
691 516 298 32 32
HincII GTy'rAC
Cuts at: 0 1167 1569
Size: 1167 402
HindIII A'AGCT_T
Cuts at: 0 19 1569
Size: 19 1550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1569
Size: 968 601
MscI TGG'CCA
Cuts at: 0 4 1569
Size: 4 1565
MslI CAynn'nnrTG
Cuts at: 0 283 1569
Size: 283 1286
MspA1I CmG'CkG
Cuts at: 0 207 606 1569
Size: 207 399 963
Fragments arranged by size:
963 399 207
NcoI C'CATG_G
Cuts at: 0 993 1569
Size: 993 576
NheI G'CTAG_C
Cuts at: 0 1392 1569
Size: 1392 177
NspI r_CATG'y
Cuts at: 0 381 1140 1371 1569
Size: 381 759 231 198
Fragments arranged by size:
759 381 231 198
PasI CC'CwG_GG
Cuts at: 0 1332 1569
Size: 1332 237
PciI A'CATG_T
Cuts at: 0 1367 1569
Size: 1367 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 1101 1569
Size: 297 804 468
Fragments arranged by size:
804 468 297
PmlI CAC'GTG
Cuts at: 0 1269 1569
Size: 1269 300
PpuMI rG'GwC_Cy
Cuts at: 0 741 759 777 1436 1569
Size: 741 18 18 659 133
Fragments arranged by size:
741 659 133 18 18
PshAI GACnn'nnGTC
Cuts at: 0 139 1569
Size: 139 1430
PvuI CG_AT'CG
Cuts at: 0 1153 1569
Size: 1153 416
PvuII CAG'CTG
Cuts at: 0 207 606 1569
Size: 207 399 963
Fragments arranged by size:
963 399 207
SacI G_AGCT'C
Cuts at: 0 254 559 1493 1569
Size: 254 305 934 76
Fragments arranged by size:
934 305 254 76
ScaI AGT'ACT
Cuts at: 0 82 199 1569
Size: 82 117 1370
Fragments arranged by size:
1370 117 82
SfcI C'TryA_G
Cuts at: 0 69 1569
Size: 69 1500
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 475 1569
Size: 475 1094
SmlI C'TyrA_G
Cuts at: 0 16 1569
Size: 16 1553
SphI G_CATG'C
Cuts at: 0 381 1140 1569
Size: 381 759 429
Fragments arranged by size:
759 429 381
StuI AGG'CCT
Cuts at: 0 1459 1569
Size: 1459 110
StyI C'CwwG_G
Cuts at: 0 993 1569
Size: 993 576
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 551 1152 1569
Size: 551 601 417
Fragments arranged by size:
601 551 417
TatI w'GTAC_w
Cuts at: 0 80 197 329 939 1088 1569
Size: 80 117 132 610 149 481
Fragments arranged by size:
610 481 149 132 117 80
Tth111I GACn'n_nGTC
Cuts at: 0 1162 1528 1569
Size: 1162 366 41
Fragments arranged by size:
1162 366 41
ZraI GAC'GTC
Cuts at: 0 849 1569
Size: 849 720
Enzymes that do cut:
AatII AcuI AfeI AflIII AlwNI ApaLI ApoI BanI
BanII BbsI BglI BglII BlpI Bme1580I BmrI BmtI
Bpu10I BpuEI BsaAI BsaHI BseRI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BstYI BtgI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NcoI NheI NspI PasI PciI PflMI PmlI
PpuMI PshAI PvuI PvuII SacI ScaI SfcI SfiI
SmlI SphI StuI StyI TaqII TatI Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BmgBI BplI BpmI BsaI BsaBI BsaWI BsaXI BseYI
BsiWI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EcoRV FalI FseI FspI
FspAI HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PfoI PmeI
PpiI PsiI PspOMI PspXI PsrI PstI RsrII SacII
SalI SanDI SapI SbfI SexAI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI XbaI
XcmI XhoI XmaI XmnI