(Linear) (Six-base) MAPSORT of: KIEE0969.seq  Check: 9890  from: 1  to: 1581



KIEE0969.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:      0     999    1581 
   Size:        999     582
 
Acc65I G'GTAC_C

Cuts at:      0     449    1581 
   Size:        449    1132
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     941    1325    1431    1460    1541    1581 
   Size:        263     678     384     106      29      81      40

  Fragments arranged by size: 

                 678     384     263     106      81      40      29
 
AflII C'TTAA_G

Cuts at:      0     306    1581 
   Size:        306    1275
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1505    1581 
   Size:       1505      76
 
ApoI r'AATT_y

Cuts at:      0    1214    1413    1581 
   Size:       1214     199     168

  Fragments arranged by size: 

                1214     199     168
 
BamHI G'GATC_C

Cuts at:      0     544    1581 
   Size:        544    1037
 
BanI G'GyrC_C

Cuts at:      0     449    1470    1581 
   Size:        449    1021     111

  Fragments arranged by size: 

                1021     449     111
 
BfrBI ATG'CAT

Cuts at:      0    1205    1581 
   Size:       1205     376
 
BlpI GC'TnA_GC

Cuts at:      0     148    1581 
   Size:        148    1433
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    1162    1581 
   Size:        355     807     419

  Fragments arranged by size: 

                 807     419     355
 
Bpu10I CC'TnA_GC

Cuts at:      0      94    1084    1581 
   Size:         94     990     497

  Fragments arranged by size: 

                 990     497      94
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     362     715    1581 
   Size:        362     353     866

  Fragments arranged by size: 

                 866     362     353
 
BseYI C'CCAG_C

Cuts at:      0     128     769    1581 
   Size:        128     641     812

  Fragments arranged by size: 

                 812     641     128
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     211    1581 
   Size:         82     129    1370

  Fragments arranged by size: 

                1370     129      82
 
BsiHKAI G_wGCw'C

Cuts at:      0     100    1581 
   Size:        100    1481
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     727    1581 
   Size:        727     854
 
Bsp1286I G_dGCh'C

Cuts at:      0     100    1581 
   Size:        100    1481
 
BspHI T'CATG_A

Cuts at:      0     713    1581 
   Size:        713     868
 
BsrFI r'CCGG_y

Cuts at:      0     706    1581 
   Size:        706     875
 
BsrGI T'GTAC_A

Cuts at:      0    1496    1581 
   Size:       1496      85
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1292    1581 
   Size:       1292     289
 
BstYI r'GATC_y

Cuts at:      0     251     544     798    1581 
   Size:        251     293     254     783

  Fragments arranged by size: 

                 783     293     254     251
 
BstZ17I GTA'TAC

Cuts at:      0    1000    1581 
   Size:       1000     581
 
BtgI C'CryG_G

Cuts at:      0     142    1581 
   Size:        142    1439
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     118    1581 
   Size:        118    1463
 
BtsI GCAGTG_nn'

Cuts at:      0     211    1581 
   Size:        211    1370
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     239     917     932    1581 
   Size:        101     138     678      15     649

  Fragments arranged by size: 

                 678     649     138     101      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     355     941    1162    1325    1431    1460    1541 
   Size:        263      92     586     221     163     106      29      81

Cuts at:   1541    1581 
   Size:         40

  Fragments arranged by size: 

                 586     263     221     163     106      92      81      40
                  29
 
EcoRV GAT'ATC

Cuts at:      0     730    1581 
   Size:        730     851
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1099    1330    1581 
   Size:       1099     231     251

  Fragments arranged by size: 

                1099     251     231
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1333    1365    1581 
   Size:       1333      32     216

  Fragments arranged by size: 

                1333     216      32
 
HindIII A'AGCT_T

Cuts at:      0    1081    1581 
   Size:       1081     500
 
KpnI G_GTAC'C

Cuts at:      0     453    1581 
   Size:        453    1128
 
MfeI C'AATT_G

Cuts at:      0     154    1581 
   Size:        154    1427
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     200    1581 
   Size:        200    1381
 
MslI CAynn'nnrTG

Cuts at:      0     860    1581 
   Size:        860     721
 
MspA1I CmG'CkG

Cuts at:      0     769    1005    1502    1581 
   Size:        769     236     497      79

  Fragments arranged by size: 

                 769     497     236      79
 
NsiI A_TGCA'T

Cuts at:      0    1207    1581 
   Size:       1207     374
 
NspI r_CATG'y

Cuts at:      0    1205    1581 
   Size:       1205     376
 
PshAI GACnn'nnGTC

Cuts at:      0     498    1581 
   Size:        498    1083
 
PsiI TTA'TAA

Cuts at:      0     820    1581 
   Size:        820     761
 
PvuII CAG'CTG

Cuts at:      0     769    1502    1581 
   Size:        769     733      79

  Fragments arranged by size: 

                 769     733      79
 
SapI GCTCTTCn'nnn_

Cuts at:      0     101    1581 
   Size:        101    1480
 
SmlI C'TyrA_G

Cuts at:      0     306    1581 
   Size:        306    1275
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     142    1581 
   Size:        142    1439
 
TatI w'GTAC_w

Cuts at:      0    1325    1496    1581 
   Size:       1325     171      85

  Fragments arranged by size: 

                1325     171      85
 
XmnI GAAnn'nnTTC

Cuts at:      0     110    1310    1581 
   Size:        110    1200     271

  Fragments arranged by size: 

                1200     271     110
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII    AlwNI     ApoI    BamHI     BanI
    BfrBI     BlpI     BpmI   Bpu10I     BsaI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BsrFI    BsrGI    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     EarI  Eco57MI    EcoRV     FalI    Hin4I
  HindIII     KpnI     MfeI     MmeI     MslI   MspA1I     NsiI     NspI
    PshAI     PsiI    PvuII     SapI     SmlI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI
    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI   BstEII   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI