(Linear) (Six-base) MAPSORT of: KIEE0902.seq  Check: 7612  from: 1  to: 453



KIEE0902.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AleI CACnn'nnGTG

Cuts at:      0      22     453 
   Size:         22     431
 
ApaI G_GGCC'C

Cuts at:      0     301     453 
   Size:        301     152
 
ApoI r'AATT_y

Cuts at:      0     123     453 
   Size:        123     330
 
AvaI C'yCGr_G

Cuts at:      0     289     453 
   Size:        289     164
 
AvrII C'CTAG_G

Cuts at:      0      57     453 
   Size:         57     396
 
BanI G'GyrC_C

Cuts at:      0     237     283     453 
   Size:        237      46     170

  Fragments arranged by size: 

                 237     170      46
 
BanII G_rGCy'C

Cuts at:      0       7     301     420     453 
   Size:          7     294     119      33

  Fragments arranged by size: 

                 294     119      33       7
 
BbvCI CC'TCA_GC

Cuts at:      0     343     453 
   Size:        343     110
 
Bme1580I G_kGCm'C

Cuts at:      0     176     288     301     453 
   Size:        176     112      13     152

  Fragments arranged by size: 

                 176     152     112      13
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     439     453 
   Size:        439      14
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     304     453 
   Size:        304     149
 
Bpu10I CC'TnA_GC

Cuts at:      0     343     453 
   Size:        343     110
 
BsaWI w'CCGG_w

Cuts at:      0     446     453 
   Size:        446       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     300     453 
   Size:        300     153
 
BseYI C'CCAG_C

Cuts at:      0     264     453 
   Size:        264     189
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     188     453 
   Size:          7     181     265

  Fragments arranged by size: 

                 265     181       7
 
BsmI GAATG_Cn'

Cuts at:      0      46     453 
   Size:         46     407
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     176     188     288     301     420     453 
   Size:          7     169      12     100      13     119      33

  Fragments arranged by size: 

                 169     119     100      33      13      12       7
 
BsrBI CCG'CTC

Cuts at:      0     425     453 
   Size:        425      28
 
BsrGI T'GTAC_A

Cuts at:      0      83     453 
   Size:         83     370
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     277     453 
   Size:        277     176
 
BstYI r'GATC_y

Cuts at:      0     379     453 
   Size:        379      74
 
Bsu36I CC'TnA_GG

Cuts at:      0     233     453 
   Size:        233     220
 
BtsI GCAGTG_nn'

Cuts at:      0     356     453 
   Size:        356      97
 
ClaI AT'CG_AT

Cuts at:      0     318     453 
   Size:        318     135
 
EcoICRI GAG'CTC

Cuts at:      0       5     453 
   Size:          5     448
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     232     453 
   Size:        232     221
 
EcoO109I rG'GnC_Cy

Cuts at:      0     297     298     453 
   Size:        297       1     155

  Fragments arranged by size: 

                 297     155       1
 
HaeII r_GCGC'y

Cuts at:      0     352     453 
   Size:        352     101
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     311     343     453 
   Size:        311      32     110

  Fragments arranged by size: 

                 311     110      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     453 
   Size:        122     331
 
MslI CAynn'nnrTG

Cuts at:      0      22     453 
   Size:         22     431
 
MspA1I CmG'CkG

Cuts at:      0     386     453 
   Size:        386      67
 
PasI CC'CwG_GG

Cuts at:      0     302     453 
   Size:        302     151
 
PspOMI G'GGCC_C

Cuts at:      0     297     453 
   Size:        297     156
 
PvuII CAG'CTG

Cuts at:      0     386     453 
   Size:        386      67
 
SacI G_AGCT'C

Cuts at:      0       7     453 
   Size:          7     446
 
StyI C'CwwG_G

Cuts at:      0      57     453 
   Size:         57     396
 
TatI w'GTAC_w

Cuts at:      0      83     453 
   Size:         83     370
 

 Enzymes that do cut:

     AleI     ApaI     ApoI     AvaI    AvrII     BanI    BanII    BbvCI
 Bme1580I     BmrI     BplI   Bpu10I    BsaWI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BsrBI    BsrGI    BstXI    BstYI   Bsu36I     BtsI
     ClaI  EcoICRI    EcoNI EcoO109I    HaeII    Hin4I     MmeI     MslI
   MspA1I     PasI   PspOMI    PvuII     SacI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI