(Linear) (Six-base) MAPSORT of: KIEE0894.seq  Check: 9113  from: 1  to: 927



KIEE0894.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     927 
   Size:        151     776
 
AleI CACnn'nnGTG

Cuts at:      0     422     742     927 
   Size:        422     320     185

  Fragments arranged by size: 

                 422     320     185
 
AlwNI CAG_nnn'CTG

Cuts at:      0     900     927 
   Size:        900      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     277     927 
   Size:        277     650
 
BbvCI CC'TCA_GC

Cuts at:      0     544     927 
   Size:        544     383
 
Bpu10I CC'TnA_GC

Cuts at:      0     544     927 
   Size:        544     383
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     664     927 
   Size:        210     454     263

  Fragments arranged by size: 

                 454     263     210
 
BsaAI yAC'GTr

Cuts at:      0     816     927 
   Size:        816     111
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      45     927 
   Size:         45     882
 
BseYI C'CCAG_C

Cuts at:      0     286     927 
   Size:        286     641
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460     616     927 
   Size:        460     156     311

  Fragments arranged by size: 

                 460     311     156
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     502     927 
   Size:        502     425
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     137     927 
   Size:        137     790
 
BsrDI GCAATG_nn'

Cuts at:      0     641     927 
   Size:        641     286
 
BstEII G'GTnAC_C

Cuts at:      0     111     927 
   Size:        111     816
 
BstYI r'GATC_y

Cuts at:      0     922     927 
   Size:        922       5
 
Bsu36I CC'TnA_GG

Cuts at:      0      65     901     927 
   Size:         65     836      26

  Fragments arranged by size: 

                 836      65      26
 
BtgI C'CryG_G

Cuts at:      0     740     927 
   Size:        740     187
 
BtsI GCAGTG_nn'

Cuts at:      0      30     927 
   Size:         30     897
 
EaeI y'GGCC_r

Cuts at:      0     143     374     927 
   Size:        143     231     553

  Fragments arranged by size: 

                 553     231     143
 
EarI CTCTTCn'nnn_

Cuts at:      0      97     576     927 
   Size:         97     479     351

  Fragments arranged by size: 

                 479     351      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     927 
   Size:        151     776
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     892     927 
   Size:        892      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     897     927 
   Size:        897      30
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      99     927 
   Size:         99     828
 
HindIII A'AGCT_T

Cuts at:      0     907     927 
   Size:        907      20
 
MfeI C'AATT_G

Cuts at:      0     824     927 
   Size:        824     103
 
MscI TGG'CCA

Cuts at:      0     145     927 
   Size:        145     782
 
MslI CAynn'nnrTG

Cuts at:      0     406     422     742     927 
   Size:        406      16     320     185

  Fragments arranged by size: 

                 406     320     185      16
 
MspA1I CmG'CkG

Cuts at:      0     338     927 
   Size:        338     589
 
PmlI CAC'GTG

Cuts at:      0     816     927 
   Size:        816     111
 
PvuII CAG'CTG

Cuts at:      0     338     927 
   Size:        338     589
 
SapI GCTCTTCn'nnn_

Cuts at:      0     576     927 
   Size:        576     351
 
SexAI A'CCwGG_T

Cuts at:      0     107     927 
   Size:        107     820
 
SfcI C'TryA_G

Cuts at:      0     671     867     927 
   Size:        671     196      60

  Fragments arranged by size: 

                 671     196      60
 
SmlI C'TyrA_G

Cuts at:      0     189     679     927 
   Size:        189     490     248

  Fragments arranged by size: 

                 490     248     189
 
StyI C'CwwG_G

Cuts at:      0     320     927 
   Size:        320     607
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     278     927 
   Size:        278     649
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     BbsI    BbvCI   Bpu10I    BpuEI    BsaAI
    BseRI    BseYI     BsgI    BsmBI    BspMI    BsrDI   BstEII    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
     FalI  HindIII     MfeI     MscI     MslI   MspA1I     PmlI    PvuII
     SapI    SexAI     SfcI     SmlI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI