Restriction map for
pF1KE0893
(Linear) (Six-base) MAPSORT of: KIEE0893.seq Check: 1983 from: 1 to: 1041
KIEE0893.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AflII C'TTAA_G
Cuts at: 0 19 1041
Size: 19 1022
AleI CACnn'nnGTG
Cuts at: 0 949 1041
Size: 949 92
ApoI r'AATT_y
Cuts at: 0 360 1031 1041
Size: 360 671 10
Fragments arranged by size:
671 360 10
BglI GCCn_nnn'nGGC
Cuts at: 0 463 1041
Size: 463 578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 1041
Size: 675 366
Bpu10I CC'TnA_GC
Cuts at: 0 173 720 1041
Size: 173 547 321
Fragments arranged by size:
547 321 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 709 1041
Size: 709 332
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 62 92 547 577 673 703 1041
Size: 62 30 455 30 96 30 338
Fragments arranged by size:
455 338 96 62 30 30 30
BseYI C'CCAG_C
Cuts at: 0 653 1041
Size: 653 388
BsiEI CG_ry'CG
Cuts at: 0 474 1041
Size: 474 567
BsiHKAI G_wGCw'C
Cuts at: 0 242 788 1041
Size: 242 546 253
Fragments arranged by size:
546 253 242
Bsp1286I G_dGCh'C
Cuts at: 0 242 788 1041
Size: 242 546 253
Fragments arranged by size:
546 253 242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 458 773 1041
Size: 224 234 315 268
Fragments arranged by size:
315 268 234 224
BsrDI GCAATG_nn'
Cuts at: 0 528 1041
Size: 528 513
BsrFI r'CCGG_y
Cuts at: 0 473 1041
Size: 473 568
BsrGI T'GTAC_A
Cuts at: 0 941 1041
Size: 941 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 534 1041
Size: 534 507
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 699 1041
Size: 108 591 342
Fragments arranged by size:
591 342 108
Bsu36I CC'TnA_GG
Cuts at: 0 138 985 1041
Size: 138 847 56
Fragments arranged by size:
847 138 56
BtgI C'CryG_G
Cuts at: 0 523 1041
Size: 523 518
BtsI GCAGTG_nn'
Cuts at: 0 70 845 1041
Size: 70 775 196
Fragments arranged by size:
775 196 70
EaeI y'GGCC_r
Cuts at: 0 251 464 482 1041
Size: 251 213 18 559
Fragments arranged by size:
559 251 213 18
EarI CTCTTCn'nnn_
Cuts at: 0 168 997 1041
Size: 168 829 44
Fragments arranged by size:
829 168 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 1041
Size: 675 366
EcoO109I rG'GnC_Cy
Cuts at: 0 893 1041
Size: 893 148
EcoRV GAT'ATC
Cuts at: 0 321 1041
Size: 321 720
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 1041
Size: 673 32 336
Fragments arranged by size:
673 336 32
HindIII A'AGCT_T
Cuts at: 0 22 1041
Size: 22 1019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1041
Size: 191 850
MscI TGG'CCA
Cuts at: 0 466 484 1041
Size: 466 18 557
Fragments arranged by size:
557 466 18
MslI CAynn'nnrTG
Cuts at: 0 283 506 937 949 1041
Size: 283 223 431 12 92
Fragments arranged by size:
431 283 223 92 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 812 1041
Size: 702 110 229
Fragments arranged by size:
702 229 110
PfoI T'CCnGG_A
Cuts at: 0 91 1041
Size: 91 950
PstI C_TGCA'G
Cuts at: 0 182 804 852 1041
Size: 182 622 48 189
Fragments arranged by size:
622 189 182 48
SfcI C'TryA_G
Cuts at: 0 123 178 800 848 978 1041
Size: 123 55 622 48 130 63
Fragments arranged by size:
622 130 123 63 55 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 463 1041
Size: 463 578
SmlI C'TyrA_G
Cuts at: 0 19 1041
Size: 19 1022
StuI AGG'CCT
Cuts at: 0 820 1041
Size: 820 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 972 1041
Size: 667 305 69
Fragments arranged by size:
667 305 69
TatI w'GTAC_w
Cuts at: 0 287 413 905 941 1041
Size: 287 126 492 36 100
Fragments arranged by size:
492 287 126 100 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 559 591 1041
Size: 62 32 465 32 450
Fragments arranged by size:
465 450 62 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 474 699 909 1041
Size: 474 225 210 132
Fragments arranged by size:
474 225 210 132
XmnI GAAnn'nnTTC
Cuts at: 0 820 970 1041
Size: 820 150 71
Fragments arranged by size:
820 150 71
Enzymes that do cut:
AflII AleI ApoI BglI BpmI Bpu10I BsaI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI
BstAPI BstXI Bsu36I BtgI BtsI EaeI EarI Eco57MI
EcoO109I EcoRV Hin4I HindIII MmeI MscI MslI PflMI
PfoI PstI SfcI SfiI SmlI StuI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaWI BseRI BsgI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstYI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI Tth111I XbaI XhoI XmaI
ZraI