(Linear) (Six-base) MAPSORT of: KIEE0889.seq  Check: 3773  from: 1  to: 966



KIEE0889.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     806     966 
   Size:        806     160
 
BamHI G'GATC_C

Cuts at:      0     724     966 
   Size:        724     242
 
BanI G'GyrC_C

Cuts at:      0     544     966 
   Size:        544     422
 
BanII G_rGCy'C

Cuts at:      0       7     900     966 
   Size:          7     893      66

  Fragments arranged by size: 

                 893      66       7
 
BfrBI ATG'CAT

Cuts at:      0     290     966 
   Size:        290     676
 
Bme1580I G_kGCm'C

Cuts at:      0     549     966 
   Size:        549     417
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661     966 
   Size:        661     305
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     966 
   Size:        187     779
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538     933     966 
   Size:        538     395      33

  Fragments arranged by size: 

                 538     395      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     775     966 
   Size:        775     191
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28     472     966 
   Size:         28     444     494

  Fragments arranged by size: 

                 494     444      28
 
BseYI C'CCAG_C

Cuts at:      0     117     436     491     966 
   Size:        117     319      55     475

  Fragments arranged by size: 

                 475     319     117      55
 
BsiHKAI G_wGCw'C

Cuts at:      0     468     966 
   Size:        468     498
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     468     549     900     966 
   Size:          7     461      81     351      66

  Fragments arranged by size: 

                 461     351      81      66       7
 
BspHI T'CATG_A

Cuts at:      0     404     966 
   Size:        404     562
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     966 
   Size:        134     832
 
BstEII G'GTnAC_C

Cuts at:      0     666     966 
   Size:        666     300
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     966 
   Size:        117     849
 
BstYI r'GATC_y

Cuts at:      0     724     966 
   Size:        724     242
 
BtgI C'CryG_G

Cuts at:      0     110     966 
   Size:        110     856
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     435     966 
   Size:        435     531
 
BtsI GCAGTG_nn'

Cuts at:      0     935     966 
   Size:        935      31
 
EaeI y'GGCC_r

Cuts at:      0      58     371     966 
   Size:         58     313     595

  Fragments arranged by size: 

                 595     313      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661     806     966 
   Size:        661     145     160

  Fragments arranged by size: 

                 661     160     145
 
EcoO109I rG'GnC_Cy

Cuts at:      0     440     966 
   Size:        440     526
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     716     748     966 
   Size:        716      32     218

  Fragments arranged by size: 

                 716     218      32
 
MscI TGG'CCA

Cuts at:      0     373     966 
   Size:        373     593
 
MslI CAynn'nnrTG

Cuts at:      0     172     349     403     430     633     847     966 
   Size:        172     177      54      27     203     214     119

  Fragments arranged by size: 

                 214     203     177     172     119      54      27
 
NdeI CA'TA_TG

Cuts at:      0     357     700     966 
   Size:        357     343     266

  Fragments arranged by size: 

                 357     343     266
 
NsiI A_TGCA'T

Cuts at:      0     292     966 
   Size:        292     674
 
NspI r_CATG'y

Cuts at:      0     102     255     966 
   Size:        102     153     711

  Fragments arranged by size: 

                 711     153     102
 
PpuMI rG'GwC_Cy

Cuts at:      0     440     966 
   Size:        440     526
 
SmlI C'TyrA_G

Cuts at:      0     553     912     966 
   Size:        553     359      54

  Fragments arranged by size: 

                 553     359      54
 
StyI C'CwwG_G

Cuts at:      0     540     692     966 
   Size:        540     152     274

  Fragments arranged by size: 

                 540     274     152
 
TatI w'GTAC_w

Cuts at:      0     176     966 
   Size:        176     790
 
XmnI GAAnn'nnTTC

Cuts at:      0     859     966 
   Size:        859     107
 

 Enzymes that do cut:

     AcuI    BamHI     BanI    BanII    BfrBI Bme1580I     BpmI   Bpu10I
    BpuEI     BsaI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     EaeI  Eco57MI
 EcoO109I    Hin4I     MscI     MslI     NdeI     NsiI     NspI    PpuMI
     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI