Restriction map for
pF1KE0888
(Linear) (Six-base) MAPSORT of: KIEE0888.seq Check: 9043 from: 1 to: 1065
KIEE0888.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 487 1065
Size: 487 578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 1065
Size: 574 491
AleI CACnn'nnGTG
Cuts at: 0 739 1065
Size: 739 326
ApoI r'AATT_y
Cuts at: 0 31 1065
Size: 31 1034
AvaI C'yCGr_G
Cuts at: 0 692 1065
Size: 692 373
AvrII C'CTAG_G
Cuts at: 0 937 1065
Size: 937 128
BanI G'GyrC_C
Cuts at: 0 619 1065
Size: 619 446
BclI T'GATC_A
Cuts at: 0 449 1065
Size: 449 616
BfrBI ATG'CAT
Cuts at: 0 1008 1065
Size: 1008 57
Bme1580I G_kGCm'C
Cuts at: 0 622 1065
Size: 622 443
BmtI G_CTAG'C
Cuts at: 0 247 1065
Size: 247 818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 445 1046 1065
Size: 25 420 601 19
Fragments arranged by size:
601 420 25 19
BsaBI GATnn'nnATC
Cuts at: 0 962 1065
Size: 962 103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 789 1043 1065
Size: 67 722 254 22
Fragments arranged by size:
722 254 67 22
BseYI C'CCAG_C
Cuts at: 0 432 793 1045 1065
Size: 432 361 252 20
Fragments arranged by size:
432 361 252 20
BsiEI CG_ry'CG
Cuts at: 0 363 1065
Size: 363 702
BsiHKAI G_wGCw'C
Cuts at: 0 412 1065
Size: 412 653
Bsp1286I G_dGCh'C
Cuts at: 0 412 622 1065
Size: 412 210 443
Fragments arranged by size:
443 412 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 487 1065
Size: 487 578
BsrFI r'CCGG_y
Cuts at: 0 508 1065
Size: 508 557
BstEII G'GTnAC_C
Cuts at: 0 108 1065
Size: 108 957
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 588 1065
Size: 105 483 477
Fragments arranged by size:
483 477 105
BstYI r'GATC_y
Cuts at: 0 680 1065
Size: 680 385
BtgI C'CryG_G
Cuts at: 0 326 1065
Size: 326 739
EaeI y'GGCC_r
Cuts at: 0 140 353 371 923 1065
Size: 140 213 18 552 142
Fragments arranged by size:
552 213 142 140 18
EarI CTCTTCn'nnn_
Cuts at: 0 85 193 1065
Size: 85 108 872
Fragments arranged by size:
872 108 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 445 574 1046 1065
Size: 25 420 129 472 19
Fragments arranged by size:
472 420 129 25 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 936 1065
Size: 936 129
EcoRI G'AATT_C
Cuts at: 0 31 1065
Size: 31 1034
HincII GTy'rAC
Cuts at: 0 207 1065
Size: 207 858
MscI TGG'CCA
Cuts at: 0 142 355 373 1065
Size: 142 213 18 692
Fragments arranged by size:
692 213 142 18
MslI CAynn'nnrTG
Cuts at: 0 172 298 613 739 847 1003 1065
Size: 172 126 315 126 108 156 62
Fragments arranged by size:
315 172 156 126 126 108 62
NaeI GCC'GGC
Cuts at: 0 510 1065
Size: 510 555
NcoI C'CATG_G
Cuts at: 0 326 1065
Size: 326 739
NgoMIV G'CCGG_C
Cuts at: 0 508 1065
Size: 508 557
NheI G'CTAG_C
Cuts at: 0 243 1065
Size: 243 822
NsiI A_TGCA'T
Cuts at: 0 1010 1065
Size: 1010 55
PasI CC'CwG_GG
Cuts at: 0 900 1065
Size: 900 165
PfoI T'CCnGG_A
Cuts at: 0 972 1065
Size: 972 93
SexAI A'CCwGG_T
Cuts at: 0 104 1065
Size: 104 961
SfcI C'TryA_G
Cuts at: 0 867 1065
Size: 867 198
StyI C'CwwG_G
Cuts at: 0 326 937 1065
Size: 326 611 128
Fragments arranged by size:
611 326 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 310 1065
Size: 186 124 755
Fragments arranged by size:
755 186 124
TatI w'GTAC_w
Cuts at: 0 176 773 1065
Size: 176 597 292
Fragments arranged by size:
597 292 176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 1065
Size: 279 786
XmnI GAAnn'nnTTC
Cuts at: 0 709 1065
Size: 709 356
Enzymes that do cut:
AarI AcuI AleI ApoI AvaI AvrII BanI BclI
BfrBI Bme1580I BmtI BpmI BsaBI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BstEII BstXI BstYI BtgI
EaeI EarI Eco57MI EcoNI EcoRI HincII MscI MslI
NaeI NcoI NgoMIV NheI NsiI PasI PfoI SexAI
SfcI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NarI
NdeI NotI NruI NspI PacI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI