Restriction map for
pF1KE0884
(Linear) (Six-base) MAPSORT of: KIEE0884.seq Check: 153 from: 1 to: 936
KIEE0884.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 0 773 936
Size: 773 163
AleI CACnn'nnGTG
Cuts at: 0 115 172 936
Size: 115 57 764
Fragments arranged by size:
764 115 57
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 241 273 549 581 936
Size: 241 32 276 32 355
Fragments arranged by size:
355 276 241 32 32
AseI AT'TA_AT
Cuts at: 0 581 936
Size: 581 355
BamHI G'GATC_C
Cuts at: 0 74 936
Size: 74 862
BanII G_rGCy'C
Cuts at: 0 412 897 936
Size: 412 485 39
Fragments arranged by size:
485 412 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 909 936
Size: 909 27
BbvCI CC'TCA_GC
Cuts at: 0 553 936
Size: 553 383
BclI T'GATC_A
Cuts at: 0 641 936
Size: 641 295
BfrBI ATG'CAT
Cuts at: 0 888 936
Size: 888 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 936
Size: 691 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 936
Size: 238 698
Bpu10I CC'TnA_GC
Cuts at: 0 62 413 553 936
Size: 62 351 140 383
Fragments arranged by size:
383 351 140 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 318 936
Size: 318 618
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 464 494 936
Size: 464 30 442
Fragments arranged by size:
464 442 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 936
Size: 472 464
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 758 936
Size: 148 610 178
Fragments arranged by size:
610 178 148
BsiHKAI G_wGCw'C
Cuts at: 0 131 936
Size: 131 805
Bsp1286I G_dGCh'C
Cuts at: 0 131 412 897 936
Size: 131 281 485 39
Fragments arranged by size:
485 281 131 39
BspHI T'CATG_A
Cuts at: 0 485 936
Size: 485 451
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 243 936
Size: 105 138 693
Fragments arranged by size:
693 138 105
BstYI r'GATC_y
Cuts at: 0 74 936
Size: 74 862
BstZ17I GTA'TAC
Cuts at: 0 774 936
Size: 774 162
Bsu36I CC'TnA_GG
Cuts at: 0 871 936
Size: 871 65
DraIII CAC_nnn'GTG
Cuts at: 0 591 936
Size: 591 345
EaeI y'GGCC_r
Cuts at: 0 140 188 371 936
Size: 140 48 183 565
Fragments arranged by size:
565 183 140 48
EarI CTCTTCn'nnn_
Cuts at: 0 217 754 936
Size: 217 537 182
Fragments arranged by size:
537 217 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 936
Size: 238 698
EcoO109I rG'GnC_Cy
Cuts at: 0 447 936
Size: 447 489
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 626 658 936
Size: 626 32 278
Fragments arranged by size:
626 278 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 936
Size: 593 343
MscI TGG'CCA
Cuts at: 0 142 190 373 936
Size: 142 48 183 563
Fragments arranged by size:
563 183 142 48
MslI CAynn'nnrTG
Cuts at: 0 115 172 403 734 823 936
Size: 115 57 231 331 89 113
Fragments arranged by size:
331 231 115 113 89 57
MspA1I CmG'CkG
Cuts at: 0 72 936
Size: 72 864
NdeI CA'TA_TG
Cuts at: 0 357 936
Size: 357 579
NsiI A_TGCA'T
Cuts at: 0 890 936
Size: 890 46
NspI r_CATG'y
Cuts at: 0 566 888 936
Size: 566 322 48
Fragments arranged by size:
566 322 48
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 9 41 936
Size: 9 32 895
Fragments arranged by size:
895 32 9
PpuMI rG'GwC_Cy
Cuts at: 0 447 936
Size: 447 489
SexAI A'CCwGG_T
Cuts at: 0 104 936
Size: 104 832
SfcI C'TryA_G
Cuts at: 0 864 936
Size: 864 72
StyI C'CwwG_G
Cuts at: 0 317 936
Size: 317 619
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 724 858 936
Size: 724 134 78
Fragments arranged by size:
724 134 78
TatI w'GTAC_w
Cuts at: 0 176 936
Size: 176 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 503 535 936
Size: 503 32 401
Fragments arranged by size:
503 401 32
XmnI GAAnn'nnTTC
Cuts at: 0 856 936
Size: 856 80
Enzymes that do cut:
AccI AleI AloI AseI BamHI BanII BbsI BbvCI
BclI BfrBI BplI BpmI Bpu10I BsaI BsaXI BseRI
BsgI BsiHKAI Bsp1286I BspHI BstXI BstYI BstZ17I Bsu36I
DraIII EaeI EarI Eco57MI EcoO109I Hin4I MmeI MscI
MslI MspA1I NdeI NsiI NspI PpiI PpuMI SexAI
SfcI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI
AsiSI AvaI AvrII BaeI BanI BbeI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpuEI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI