Restriction map for
pF1KE0883
(Linear) (Six-base) MAPSORT of: KIEE0883.seq Check: 2505 from: 1 to: 942
KIEE0883.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 942
Size: 727 215
AatII G_ACGT'C
Cuts at: 0 575 942
Size: 575 367
AccI GT'mk_AC
Cuts at: 0 223 942
Size: 223 719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 886 942
Size: 697 189 56
Fragments arranged by size:
697 189 56
AhdI GACnn_n'nnGTC
Cuts at: 0 816 942
Size: 816 126
ApaLI G'TGCA_C
Cuts at: 0 921 942
Size: 921 21
ApoI r'AATT_y
Cuts at: 0 31 942
Size: 31 911
BanI G'GyrC_C
Cuts at: 0 262 942
Size: 262 680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 747 781 942
Size: 747 34 161
Fragments arranged by size:
747 161 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 649 942
Size: 649 293
BclI T'GATC_A
Cuts at: 0 590 942
Size: 590 352
BlpI GC'TnA_GC
Cuts at: 0 57 942
Size: 57 885
Bme1580I G_kGCm'C
Cuts at: 0 925 942
Size: 925 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 830 942
Size: 830 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 942
Size: 901 41
BsaBI GATnn'nnATC
Cuts at: 0 542 942
Size: 542 400
BsaHI Gr'CG_yC
Cuts at: 0 572 942
Size: 572 370
BseYI C'CCAG_C
Cuts at: 0 296 942
Size: 296 646
BsiHKAI G_wGCw'C
Cuts at: 0 89 925 942
Size: 89 836 17
Fragments arranged by size:
836 89 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 579 942
Size: 579 363
Bsp1286I G_dGCh'C
Cuts at: 0 89 925 942
Size: 89 836 17
Fragments arranged by size:
836 89 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 942
Size: 727 215
BsrDI GCAATG_nn'
Cuts at: 0 134 942
Size: 134 808
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 942
Size: 117 825
Bsu36I CC'TnA_GG
Cuts at: 0 874 942
Size: 874 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 750 942
Size: 750 192
EarI CTCTTCn'nnn_
Cuts at: 0 307 886 942
Size: 307 579 56
Fragments arranged by size:
579 307 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 886 942
Size: 697 189 56
Fragments arranged by size:
697 189 56
EcoRI G'AATT_C
Cuts at: 0 31 942
Size: 31 911
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 840 872 942
Size: 802 32 6 32 70
Fragments arranged by size:
802 70 32 32 6
HincII GTy'rAC
Cuts at: 0 207 224 821 942
Size: 207 17 597 121
Fragments arranged by size:
597 207 121 17
MspA1I CmG'CkG
Cuts at: 0 495 725 942
Size: 495 230 217
Fragments arranged by size:
495 230 217
NdeI CA'TA_TG
Cuts at: 0 357 797 942
Size: 357 440 145
Fragments arranged by size:
440 357 145
NspI r_CATG'y
Cuts at: 0 129 942
Size: 129 813
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 942
Size: 432 510
PshAI GACnn'nnGTC
Cuts at: 0 229 942
Size: 229 713
PstI C_TGCA'G
Cuts at: 0 557 724 942
Size: 557 167 218
Fragments arranged by size:
557 218 167
PvuII CAG'CTG
Cuts at: 0 495 725 942
Size: 495 230 217
Fragments arranged by size:
495 230 217
SalI G'TCGA_C
Cuts at: 0 222 942
Size: 222 720
SapI GCTCTTCn'nnn_
Cuts at: 0 307 942
Size: 307 635
SfcI C'TryA_G
Cuts at: 0 227 553 720 942
Size: 227 326 167 222
Fragments arranged by size:
326 227 222 167
SmlI C'TyrA_G
Cuts at: 0 916 942
Size: 916 26
StyI C'CwwG_G
Cuts at: 0 116 942
Size: 116 826
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 310 942
Size: 310 632
TatI w'GTAC_w
Cuts at: 0 176 942
Size: 176 766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 151 183 942
Size: 151 32 759
Fragments arranged by size:
759 151 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 433 942
Size: 433 509
XmnI GAAnn'nnTTC
Cuts at: 0 859 942
Size: 859 83
ZraI GAC'GTC
Cuts at: 0 573 942
Size: 573 369
Enzymes that do cut:
AarI AatII AccI AcuI AhdI ApaLI ApoI BanI
BcgI BciVI BclI BlpI Bme1580I BplI BpuEI BsaBI
BsaHI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstXI
Bsu36I BtgZI EarI Eco57MI EcoRI Hin4I HincII MspA1I
NdeI NspI PflMI PshAI PstI PvuII SalI SapI
SfcI SmlI StyI TaqII TatI TstI XcmI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BfrBI BglI
BglII BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI
BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI