Restriction map for
pF1KE0881
(Linear) (Six-base) MAPSORT of: KIEE0881.seq Check: 5465 from: 1 to: 927
KIEE0881.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AatII G_ACGT'C
Cuts at: 0 575 927
Size: 575 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 891 927
Size: 574 317 36
Fragments arranged by size:
574 317 36
AvrII C'CTAG_G
Cuts at: 0 600 927
Size: 600 327
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 663 927
Size: 69 594 264
Fragments arranged by size:
594 264 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 433 927
Size: 433 494
BfrBI ATG'CAT
Cuts at: 0 635 927
Size: 635 292
BsaHI Gr'CG_yC
Cuts at: 0 572 927
Size: 572 355
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 912 927
Size: 912 15
BseYI C'CCAG_C
Cuts at: 0 436 840 927
Size: 436 404 87
Fragments arranged by size:
436 404 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 927
Size: 696 231
BsiEI CG_ry'CG
Cuts at: 0 363 927
Size: 363 564
BsiHKAI G_wGCw'C
Cuts at: 0 468 927
Size: 468 459
Bsp1286I G_dGCh'C
Cuts at: 0 468 927
Size: 468 459
BspHI T'CATG_A
Cuts at: 0 308 927
Size: 308 619
BsrDI GCAATG_nn'
Cuts at: 0 356 927
Size: 356 571
Bsu36I CC'TnA_GG
Cuts at: 0 236 927
Size: 236 691
BtgI C'CryG_G
Cuts at: 0 412 927
Size: 412 515
BtsI GCAGTG_nn'
Cuts at: 0 815 839 927
Size: 815 24 88
Fragments arranged by size:
815 88 24
EaeI y'GGCC_r
Cuts at: 0 140 245 320 371 927
Size: 140 105 75 51 556
Fragments arranged by size:
556 140 105 75 51
EarI CTCTTCn'nnn_
Cuts at: 0 910 927
Size: 910 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 766 927
Size: 766 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 891 927
Size: 574 317 36
Fragments arranged by size:
574 317 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 927
Size: 108 32 787
Fragments arranged by size:
787 108 32
HincII GTy'rAC
Cuts at: 0 207 927
Size: 207 720
MscI TGG'CCA
Cuts at: 0 142 247 322 373 927
Size: 142 105 75 51 554
Fragments arranged by size:
554 142 105 75 51
MslI CAynn'nnrTG
Cuts at: 0 581 630 927
Size: 581 49 297
Fragments arranged by size:
581 297 49
MspA1I CmG'CkG
Cuts at: 0 380 927
Size: 380 547
NsiI A_TGCA'T
Cuts at: 0 637 927
Size: 637 290
NspI r_CATG'y
Cuts at: 0 771 927
Size: 771 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 927
Size: 258 669
PvuI CG_AT'CG
Cuts at: 0 363 927
Size: 363 564
PvuII CAG'CTG
Cuts at: 0 380 927
Size: 380 547
SfcI C'TryA_G
Cuts at: 0 861 895 927
Size: 861 34 32
Fragments arranged by size:
861 34 32
SpeI A'CTAG_T
Cuts at: 0 52 927
Size: 52 875
StyI C'CwwG_G
Cuts at: 0 200 317 323 341 600 927
Size: 200 117 6 18 259 327
Fragments arranged by size:
327 259 200 117 18 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 927
Size: 435 492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 255 927
Size: 198 57 672
Fragments arranged by size:
672 198 57
XmnI GAAnn'nnTTC
Cuts at: 0 904 927
Size: 904 23
ZraI GAC'GTC
Cuts at: 0 573 927
Size: 573 354
Enzymes that do cut:
AatII AcuI AvrII BbsI BciVI BfrBI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrDI Bsu36I
BtgI BtsI EaeI EarI EciI Eco57MI Hin4I HincII
MscI MslI MspA1I NsiI NspI PflMI PvuI PvuII
SfcI SpeI StyI TaqII XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI