(Linear) (Six-base) MAPSORT of: KIEE0878.seq  Check: 6954  from: 1  to: 921



KIEE0878.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AleI CACnn'nnGTG

Cuts at:      0     730     921 
   Size:        730     191
 
ApaLI G'TGCA_C

Cuts at:      0     463     921 
   Size:        463     458
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     682     921 
   Size:        682     239
 
BlpI GC'TnA_GC

Cuts at:      0     424     921 
   Size:        424     497
 
Bme1580I G_kGCm'C

Cuts at:      0     467     921 
   Size:        467     454
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     921 
   Size:         43     878
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     314     921 
   Size:        314     607
 
BseYI C'CCAG_C

Cuts at:      0     234     287     921 
   Size:        234      53     634

  Fragments arranged by size: 

                 634     234      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     800     921 
   Size:        192     608     121

  Fragments arranged by size: 

                 608     192     121
 
BsiHKAI G_wGCw'C

Cuts at:      0     467     921 
   Size:        467     454
 
Bsp1286I G_dGCh'C

Cuts at:      0     467     921 
   Size:        467     454
 
BspHI T'CATG_A

Cuts at:      0     479     921 
   Size:        479     442
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     640     921 
   Size:        640     281
 
BsrDI GCAATG_nn'

Cuts at:      0     128     538     921 
   Size:        128     410     383

  Fragments arranged by size: 

                 410     383     128
 
BsrGI T'GTAC_A

Cuts at:      0     857     921 
   Size:        857      64
 
BstEII G'GTnAC_C

Cuts at:      0     732     921 
   Size:        732     189
 
BtgI C'CryG_G

Cuts at:      0     341     921 
   Size:        341     580
 
EaeI y'GGCC_r

Cuts at:      0     362     608     921 
   Size:        362     246     313

  Fragments arranged by size: 

                 362     313     246
 
EarI CTCTTCn'nnn_

Cuts at:      0     298     383     921 
   Size:        298      85     538

  Fragments arranged by size: 

                 538     298      85
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     760     921 
   Size:        760     161
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     921 
   Size:         43     878
 
HincII GTy'rAC

Cuts at:      0     488     921 
   Size:        488     433
 
MscI TGG'CCA

Cuts at:      0     364     610     921 
   Size:        364     246     311

  Fragments arranged by size: 

                 364     311     246
 
MslI CAynn'nnrTG

Cuts at:      0     410     730     921 
   Size:        410     320     191

  Fragments arranged by size: 

                 410     320     191
 
NcoI C'CATG_G

Cuts at:      0     341     921 
   Size:        341     580
 
NdeI CA'TA_TG

Cuts at:      0     270     407     921 
   Size:        270     137     514

  Fragments arranged by size: 

                 514     270     137
 
NspI r_CATG'y

Cuts at:      0     120     285     713     921 
   Size:        120     165     428     208

  Fragments arranged by size: 

                 428     208     165     120
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     458     921 
   Size:        458     463
 
SapI GCTCTTCn'nnn_

Cuts at:      0     298     921 
   Size:        298     623
 
ScaI AGT'ACT

Cuts at:      0     390     921 
   Size:        390     531
 
SfcI C'TryA_G

Cuts at:      0     555     921 
   Size:        555     366
 
StuI AGG'CCT

Cuts at:      0     700     921 
   Size:        700     221
 
StyI C'CwwG_G

Cuts at:      0     341     921 
   Size:        341     580
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     450     921 
   Size:        450     471
 
TatI w'GTAC_w

Cuts at:      0     388     857     921 
   Size:        388     469      64

  Fragments arranged by size: 

                 469     388      64
 
XmnI GAAnn'nnTTC

Cuts at:      0     700     921 
   Size:        700     221
 

 Enzymes that do cut:

     AleI    ApaLI    BciVI     BlpI Bme1580I     BpmI     BsaI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI   BstEII
     BtgI     EaeI     EarI     EciI  Eco57MI   HincII     MscI     MslI
     NcoI     NdeI     NspI    PflMI     SapI     ScaI     SfcI     StuI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI