Restriction map for
pF1KE0877
(Linear) (Six-base) MAPSORT of: KIEE0877.seq Check: 8193 from: 1 to: 957
KIEE0877.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 170 957
Size: 170 787
AccI GT'mk_AC
Cuts at: 0 873 957
Size: 873 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 957
Size: 799 158
AgeI A'CCGG_T
Cuts at: 0 362 957
Size: 362 595
AhdI GACnn_n'nnGTC
Cuts at: 0 455 957
Size: 455 502
AleI CACnn'nnGTG
Cuts at: 0 298 957
Size: 298 659
ApaLI G'TGCA_C
Cuts at: 0 720 957
Size: 720 237
ApoI r'AATT_y
Cuts at: 0 539 814 957
Size: 539 275 143
Fragments arranged by size:
539 275 143
BanII G_rGCy'C
Cuts at: 0 325 957
Size: 325 632
BbvCI CC'TCA_GC
Cuts at: 0 187 957
Size: 187 770
BciVI GTATCCnnnnn_n'
Cuts at: 0 682 957
Size: 682 275
BclI T'GATC_A
Cuts at: 0 107 602 957
Size: 107 495 355
Fragments arranged by size:
495 355 107
BfrBI ATG'CAT
Cuts at: 0 655 957
Size: 655 302
Bme1580I G_kGCm'C
Cuts at: 0 724 957
Size: 724 233
Bpu10I CC'TnA_GC
Cuts at: 0 187 957
Size: 187 770
BsaBI GATnn'nnATC
Cuts at: 0 139 957
Size: 139 818
BsaWI w'CCGG_w
Cuts at: 0 362 957
Size: 362 595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 405 435 602 632 957
Size: 405 30 167 30 325
Fragments arranged by size:
405 325 167 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 957
Size: 210 747
BseYI C'CCAG_C
Cuts at: 0 117 957
Size: 117 840
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 445 604 957
Size: 148 297 159 353
Fragments arranged by size:
353 297 159 148
BsiHKAI G_wGCw'C
Cuts at: 0 341 724 957
Size: 341 383 233
Fragments arranged by size:
383 341 233
Bsp1286I G_dGCh'C
Cuts at: 0 325 341 724 957
Size: 325 16 383 233
Fragments arranged by size:
383 325 233 16
BspHI T'CATG_A
Cuts at: 0 404 957
Size: 404 553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 957
Size: 170 787
BsrFI r'CCGG_y
Cuts at: 0 362 957
Size: 362 595
BtgI C'CryG_G
Cuts at: 0 317 467 957
Size: 317 150 490
Fragments arranged by size:
490 317 150
BtsI GCAGTG_nn'
Cuts at: 0 461 957
Size: 461 496
DraIII CAC_nnn'GTG
Cuts at: 0 332 957
Size: 332 625
EaeI y'GGCC_r
Cuts at: 0 371 545 957
Size: 371 174 412
Fragments arranged by size:
412 371 174
EarI CTCTTCn'nnn_
Cuts at: 0 187 228 957
Size: 187 41 729
Fragments arranged by size:
729 187 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 957
Size: 799 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 441 473 906 938 957
Size: 383 32 26 32 433 32 19
Fragments arranged by size:
433 383 32 32 32 26 19
MscI TGG'CCA
Cuts at: 0 373 547 957
Size: 373 174 410
Fragments arranged by size:
410 373 174
MslI CAynn'nnrTG
Cuts at: 0 172 298 466 598 628 957
Size: 172 126 168 132 30 329
Fragments arranged by size:
329 172 168 132 126 30
MspA1I CmG'CkG
Cuts at: 0 436 790 957
Size: 436 354 167
Fragments arranged by size:
436 354 167
NcoI C'CATG_G
Cuts at: 0 317 467 957
Size: 317 150 490
Fragments arranged by size:
490 317 150
NdeI CA'TA_TG
Cuts at: 0 357 957
Size: 357 600
NsiI A_TGCA'T
Cuts at: 0 657 957
Size: 657 300
PflMI CCAn_nnn'nTGG
Cuts at: 0 735 957
Size: 735 222
PfoI T'CCnGG_A
Cuts at: 0 442 957
Size: 442 515
PvuII CAG'CTG
Cuts at: 0 436 957
Size: 436 521
SfcI C'TryA_G
Cuts at: 0 791 867 957
Size: 791 76 90
Fragments arranged by size:
791 90 76
StyI C'CwwG_G
Cuts at: 0 317 467 957
Size: 317 150 490
Fragments arranged by size:
490 317 150
TatI w'GTAC_w
Cuts at: 0 609 957
Size: 609 348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 405 437 957
Size: 405 32 520
Fragments arranged by size:
520 405 32
XbaI T'CTAG_A
Cuts at: 0 150 957
Size: 150 807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 957
Size: 555 402
Enzymes that do cut:
AarI AccI AcuI AgeI AhdI AleI ApaLI ApoI
BanII BbvCI BciVI BclI BfrBI Bme1580I Bpu10I BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrFI BtgI BtsI DraIII EaeI EarI Eco57MI
Hin4I MscI MslI MspA1I NcoI NdeI NsiI PflMI
PfoI PvuII SfcI StyI TatI TstI XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AlfI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XhoI XmaI XmnI ZraI