Restriction map for
pF1KE0871
(Linear) (Six-base) MAPSORT of: KIEE0871.seq Check: 8743 from: 1 to: 972
KIEE0871.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 0 17 972
Size: 17 955
AflII C'TTAA_G
Cuts at: 0 21 972
Size: 21 951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 195 443 972
Size: 195 248 529
Fragments arranged by size:
529 248 195
ApoI r'AATT_y
Cuts at: 0 222 956 966 972
Size: 222 734 10 6
Fragments arranged by size:
734 222 10 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 406 439 972
Size: 406 33 533
Fragments arranged by size:
533 406 33
BamHI G'GATC_C
Cuts at: 0 824 972
Size: 824 148
BanII G_rGCy'C
Cuts at: 0 640 972
Size: 640 332
BciVI GTATCCnnnnn_n'
Cuts at: 0 487 972
Size: 487 485
BclI T'GATC_A
Cuts at: 0 552 972
Size: 552 420
BglII A'GATC_T
Cuts at: 0 239 972
Size: 239 733
Bme1580I G_kGCm'C
Cuts at: 0 600 972
Size: 600 372
BmrI ACTGGGnnnn_n'
Cuts at: 0 358 972
Size: 358 614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 972
Size: 310 662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 972
Size: 256 716
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 271 301 972
Size: 271 30 671
Fragments arranged by size:
671 271 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 866 972
Size: 866 106
BseYI C'CCAG_C
Cuts at: 0 147 216 600 972
Size: 147 69 384 372
Fragments arranged by size:
384 372 147 69
BsiHKAI G_wGCw'C
Cuts at: 0 640 972
Size: 640 332
Bsp1286I G_dGCh'C
Cuts at: 0 600 640 972
Size: 600 40 332
Fragments arranged by size:
600 332 40
BspHI T'CATG_A
Cuts at: 0 434 972
Size: 434 538
BsrGI T'GTAC_A
Cuts at: 0 665 972
Size: 665 307
BstYI r'GATC_y
Cuts at: 0 239 824 845 972
Size: 239 585 21 127
Fragments arranged by size:
585 239 127 21
BtsI GCAGTG_nn'
Cuts at: 0 179 415 972
Size: 179 236 557
Fragments arranged by size:
557 236 179
ClaI AT'CG_AT
Cuts at: 0 393 972
Size: 393 579
DraIII CAC_nnn'GTG
Cuts at: 0 54 972
Size: 54 918
EaeI y'GGCC_r
Cuts at: 0 401 972
Size: 401 571
EarI CTCTTCn'nnn_
Cuts at: 0 115 908 972
Size: 115 793 64
Fragments arranged by size:
793 115 64
EcoICRI GAG'CTC
Cuts at: 0 638 972
Size: 638 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 972
Size: 310 662
EcoO109I rG'GnC_Cy
Cuts at: 0 711 972
Size: 711 261
EcoRI G'AATT_C
Cuts at: 0 956 972
Size: 956 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 668 700 885 917 945 972
Size: 149 32 487 32 185 32 28 27
Fragments arranged by size:
487 185 149 32 32 32 28 27
HincII GTy'rAC
Cuts at: 0 279 972
Size: 279 693
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 972
Size: 709 263
MscI TGG'CCA
Cuts at: 0 403 972
Size: 403 569
MslI CAynn'nnrTG
Cuts at: 0 202 661 799 972
Size: 202 459 138 173
Fragments arranged by size:
459 202 173 138
MspA1I CmG'CkG
Cuts at: 0 700 724 972
Size: 700 24 248
Fragments arranged by size:
700 248 24
NdeI CA'TA_TG
Cuts at: 0 819 972
Size: 819 153
NspI r_CATG'y
Cuts at: 0 273 972
Size: 273 699
PpuMI rG'GwC_Cy
Cuts at: 0 711 972
Size: 711 261
PstI C_TGCA'G
Cuts at: 0 422 723 972
Size: 422 301 249
Fragments arranged by size:
422 301 249
PvuII CAG'CTG
Cuts at: 0 700 724 972
Size: 700 24 248
Fragments arranged by size:
700 248 24
SacI G_AGCT'C
Cuts at: 0 640 972
Size: 640 332
SfcI C'TryA_G
Cuts at: 0 418 719 972
Size: 418 301 253
Fragments arranged by size:
418 301 253
SmlI C'TyrA_G
Cuts at: 0 21 235 972
Size: 21 214 737
Fragments arranged by size:
737 214 21
SpeI A'CTAG_T
Cuts at: 0 658 972
Size: 658 314
StyI C'CwwG_G
Cuts at: 0 296 489 972
Size: 296 193 483
Fragments arranged by size:
483 296 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 305 428 972
Size: 305 123 544
Fragments arranged by size:
544 305 123
TatI w'GTAC_w
Cuts at: 0 206 665 972
Size: 206 459 307
Fragments arranged by size:
459 307 206
Enzymes that do cut:
AccI AflII AlfI ApoI BaeI BamHI BanII BciVI
BclI BglII Bme1580I BmrI BpmI BpuEI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspHI BsrGI BstYI BtsI ClaI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I
HincII MmeI MscI MslI MspA1I NdeI NspI PpuMI
PstI PvuII SacI SfcI SmlI SpeI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BanI BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
CspCI DraI DrdI EagI EciI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI