(Linear) (Six-base) MAPSORT of: KIEE0867.seq  Check: 6108  from: 1  to: 927



KIEE0867.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AflIII A'CryG_T

Cuts at:      0     579     927 
   Size:        579     348
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     927 
   Size:         75     852
 
ApoI r'AATT_y

Cuts at:      0     922     927 
   Size:        922       5
 
BamHI G'GATC_C

Cuts at:      0     683     927 
   Size:        683     244
 
BanI G'GyrC_C

Cuts at:      0     295     701     927 
   Size:        295     406     226

  Fragments arranged by size: 

                 406     295     226
 
BbeI G_GCGC'C

Cuts at:      0     705     927 
   Size:        705     222
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      81     309     651     927 
   Size:         81     228     342     276

  Fragments arranged by size: 

                 342     276     228      81
 
BglII A'GATC_T

Cuts at:      0     521     927 
   Size:        521     406
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     782     927 
   Size:        782     145
 
Bpu10I CC'TnA_GC

Cuts at:      0     622     927 
   Size:        622     305
 
BsaBI GATnn'nnATC

Cuts at:      0     646     927 
   Size:        646     281
 
BsaHI Gr'CG_yC

Cuts at:      0     702     927 
   Size:        702     225
 
BsaWI w'CCGG_w

Cuts at:      0     686     927 
   Size:        686     241
 
BseYI C'CCAG_C

Cuts at:      0     453     927 
   Size:        453     474
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     747     927 
   Size:        590     157     180

  Fragments arranged by size: 

                 590     180     157
 
BspHI T'CATG_A

Cuts at:      0     407     927 
   Size:        407     520
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     730     927 
   Size:        730     197
 
BstYI r'GATC_y

Cuts at:      0     521     683     927 
   Size:        521     162     244

  Fragments arranged by size: 

                 521     244     162
 
Bsu36I CC'TnA_GG

Cuts at:      0      62     904     927 
   Size:         62     842      23

  Fragments arranged by size: 

                 842      62      23
 
BtsI GCAGTG_nn'

Cuts at:      0     469     927 
   Size:        469     458
 
DraI TTT'AAA

Cuts at:      0     857     927 
   Size:        857      70
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      64     927 
   Size:         64     863
 
EarI CTCTTCn'nnn_

Cuts at:      0     732     927 
   Size:        732     195
 
HaeII r_GCGC'y

Cuts at:      0     705     927 
   Size:        705     222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      87     119     386     418     927 
   Size:         87      32     267      32     509

  Fragments arranged by size: 

                 509     267      87      32      32
 
KasI G'GCGC_C

Cuts at:      0     701     927 
   Size:        701     226
 
MfeI C'AATT_G

Cuts at:      0     131     927 
   Size:        131     796
 
MluI A'CGCG_T

Cuts at:      0     579     927 
   Size:        579     348
 
MslI CAynn'nnrTG

Cuts at:      0     172     301     515     739     927 
   Size:        172     129     214     224     188

  Fragments arranged by size: 

                 224     214     188     172     129
 
NarI GG'CG_CC

Cuts at:      0     702     927 
   Size:        702     225
 
NspI r_CATG'y

Cuts at:      0     546     927 
   Size:        546     381
 
PstI C_TGCA'G

Cuts at:      0     552     755     927 
   Size:        552     203     172

  Fragments arranged by size: 

                 552     203     172
 
SapI GCTCTTCn'nnn_

Cuts at:      0     732     927 
   Size:        732     195
 
SfcI C'TryA_G

Cuts at:      0     548     751     870     927 
   Size:        548     203     119      57

  Fragments arranged by size: 

                 548     203     119      57
 
SfoI GGC'GCC

Cuts at:      0     703     927 
   Size:        703     224
 
StyI C'CwwG_G

Cuts at:      0     242     446     927 
   Size:        242     204     481

  Fragments arranged by size: 

                 481     242     204
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     313     927 
   Size:        313     614
 
TatI w'GTAC_w

Cuts at:      0     176     927 
   Size:        176     751
 
XbaI T'CTAG_A

Cuts at:      0     204     927 
   Size:        204     723
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     234     927 
   Size:        234     693
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI    BamHI     BanI     BbeI     BbsI    BglII
     BmrI   Bpu10I    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BspHI
    BspMI    BstYI   Bsu36I     BtsI     DraI     DrdI     EarI    HaeII
    Hin4I     KasI     MfeI     MluI     MslI     NarI     NspI     PstI
     SapI     SfcI     SfoI     StyI    TaqII     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI   DraIII     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MmeI
     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XhoI     XmaI     XmnI     ZraI