Restriction map for
pF1KE0865
(Linear) (Six-base) MAPSORT of: KIEE0865.seq Check: 7922 from: 1 to: 1005
KIEE0865.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 465 787 1005
Size: 465 322 218
Fragments arranged by size:
465 322 218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1005
Size: 757 248
AleI CACnn'nnGTG
Cuts at: 0 794 1005
Size: 794 211
ApoI r'AATT_y
Cuts at: 0 8 91 125 540 1005
Size: 8 83 34 415 465
Fragments arranged by size:
465 415 83 34 8
BbvCI CC'TCA_GC
Cuts at: 0 747 752 1005
Size: 747 5 253
Fragments arranged by size:
747 253 5
BglII A'GATC_T
Cuts at: 0 59 1005
Size: 59 946
Bpu10I CC'TnA_GC
Cuts at: 0 747 752 1005
Size: 747 5 253
Fragments arranged by size:
747 253 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 262 1005
Size: 262 743
BsaBI GATnn'nnATC
Cuts at: 0 304 1005
Size: 304 701
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 659 689 1005
Size: 659 30 316
Fragments arranged by size:
659 316 30
BseYI C'CCAG_C
Cuts at: 0 102 1005
Size: 102 903
BsiHKAI G_wGCw'C
Cuts at: 0 354 1005
Size: 354 651
BsmI GAATG_Cn'
Cuts at: 0 251 719 1005
Size: 251 468 286
Fragments arranged by size:
468 286 251
Bsp1286I G_dGCh'C
Cuts at: 0 354 1005
Size: 354 651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 465 787 1005
Size: 465 322 218
Fragments arranged by size:
465 322 218
BsrDI GCAATG_nn'
Cuts at: 0 189 1005
Size: 189 816
BstEII G'GTnAC_C
Cuts at: 0 84 1005
Size: 84 921
BstYI r'GATC_y
Cuts at: 0 59 1005
Size: 59 946
Bsu36I CC'TnA_GG
Cuts at: 0 838 1005
Size: 838 167
BtgI C'CryG_G
Cuts at: 0 80 920 1005
Size: 80 840 85
Fragments arranged by size:
840 85 80
EaeI y'GGCC_r
Cuts at: 0 323 1005
Size: 323 682
EarI CTCTTCn'nnn_
Cuts at: 0 359 1005
Size: 359 646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1005
Size: 757 248
EcoRI G'AATT_C
Cuts at: 0 91 1005
Size: 91 914
HaeII r_GCGC'y
Cuts at: 0 572 1005
Size: 572 433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 888 920 1005
Size: 888 32 85
Fragments arranged by size:
888 85 32
HincII GTy'rAC
Cuts at: 0 642 1005
Size: 642 363
HpaI GTT'AAC
Cuts at: 0 642 1005
Size: 642 363
MfeI C'AATT_G
Cuts at: 0 506 1005
Size: 506 499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 975 1005
Size: 887 88 30
Fragments arranged by size:
887 88 30
MscI TGG'CCA
Cuts at: 0 325 1005
Size: 325 680
MslI CAynn'nnrTG
Cuts at: 0 232 463 794 1005
Size: 232 231 331 211
Fragments arranged by size:
331 232 231 211
MspA1I CmG'CkG
Cuts at: 0 132 496 751 1005
Size: 132 364 255 254
Fragments arranged by size:
364 255 254 132
NcoI C'CATG_G
Cuts at: 0 80 920 1005
Size: 80 840 85
Fragments arranged by size:
840 85 80
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 668 700 1005
Size: 668 32 305
Fragments arranged by size:
668 305 32
PvuII CAG'CTG
Cuts at: 0 132 496 751 1005
Size: 132 364 255 254
Fragments arranged by size:
364 255 254 132
SapI GCTCTTCn'nnn_
Cuts at: 0 359 1005
Size: 359 646
SfcI C'TryA_G
Cuts at: 0 845 1005
Size: 845 160
StuI AGG'CCT
Cuts at: 0 843 1005
Size: 843 162
StyI C'CwwG_G
Cuts at: 0 80 117 246 372 920 1005
Size: 80 37 129 126 548 85
Fragments arranged by size:
548 129 126 85 80 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 394 1005
Size: 394 611
TatI w'GTAC_w
Cuts at: 0 236 675 1005
Size: 236 439 330
Fragments arranged by size:
439 330 236
Enzymes that do cut:
AarI AcuI AleI ApoI BbvCI BglII Bpu10I BsaI
BsaBI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BstEII BstYI Bsu36I BtgI EaeI EarI Eco57MI EcoRI
HaeII Hin4I HincII HpaI MfeI MmeI MscI MslI
MspA1I NcoI PsrI PvuII SapI SfcI StuI StyI
TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI BsaHI
BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HindIII KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI