Restriction map for
pF1KE0864
(Linear) (Six-base) MAPSORT of: KIEE0864.seq Check: 1190 from: 1 to: 957
KIEE0864.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 742 957
Size: 742 215
AccI GT'mk_AC
Cuts at: 0 845 957
Size: 845 112
AflIII A'CryG_T
Cuts at: 0 668 957
Size: 668 289
AleI CACnn'nnGTG
Cuts at: 0 235 957
Size: 235 722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 457 646 957
Size: 457 189 311
Fragments arranged by size:
457 311 189
AlwNI CAG_nnn'CTG
Cuts at: 0 73 444 946 957
Size: 73 371 502 11
Fragments arranged by size:
502 371 73 11
ApaI G_GGCC'C
Cuts at: 0 276 309 456 957
Size: 276 33 147 501
Fragments arranged by size:
501 276 147 33
ApaLI G'TGCA_C
Cuts at: 0 159 432 595 957
Size: 159 273 163 362
Fragments arranged by size:
362 273 163 159
ApoI r'AATT_y
Cuts at: 0 76 957
Size: 76 881
BanII G_rGCy'C
Cuts at: 0 276 309 456 957
Size: 276 33 147 501
Fragments arranged by size:
501 276 147 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 12 957
Size: 12 945
Bme1580I G_kGCm'C
Cuts at: 0 163 276 309 436 456 599 957
Size: 163 113 33 127 20 143 358
Fragments arranged by size:
358 163 143 127 113 33 20
Bpu10I CC'TnA_GC
Cuts at: 0 691 957
Size: 691 266
BsaAI yAC'GTr
Cuts at: 0 669 957
Size: 669 288
BsaHI Gr'CG_yC
Cuts at: 0 950 957
Size: 950 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 100 933 957
Size: 97 3 833 24
Fragments arranged by size:
833 97 24 3
BseYI C'CCAG_C
Cuts at: 0 444 957
Size: 444 513
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 957
Size: 385 572
BsiHKAI G_wGCw'C
Cuts at: 0 163 436 599 915 957
Size: 163 273 163 316 42
Fragments arranged by size:
316 273 163 163 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 666 957
Size: 666 291
Bsp1286I G_dGCh'C
Cuts at: 0 163 276 309 436 456 599 915 957
Size: 163 113 33 127 20 143 316 42
Fragments arranged by size:
316 163 143 127 113 42 33 20
BspHI T'CATG_A
Cuts at: 0 416 957
Size: 416 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 742 800 957
Size: 742 58 157
Fragments arranged by size:
742 157 58
BssSI C'ACGA_G
Cuts at: 0 628 784 957
Size: 628 156 173
Fragments arranged by size:
628 173 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 291 957
Size: 267 24 666
Fragments arranged by size:
666 267 24
Bsu36I CC'TnA_GG
Cuts at: 0 889 957
Size: 889 68
BtgI C'CryG_G
Cuts at: 0 236 752 957
Size: 236 516 205
Fragments arranged by size:
516 236 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 566 957
Size: 566 391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 27 62 957
Size: 27 35 895
Fragments arranged by size:
895 35 27
EaeI y'GGCC_r
Cuts at: 0 519 957
Size: 519 438
EarI CTCTTCn'nnn_
Cuts at: 0 97 106 475 957
Size: 97 9 369 482
Fragments arranged by size:
482 369 97 9
EcoO109I rG'GnC_Cy
Cuts at: 0 272 305 452 453 957
Size: 272 33 147 1 504
Fragments arranged by size:
504 272 147 33 1
EcoRI G'AATT_C
Cuts at: 0 76 957
Size: 76 881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 284 957
Size: 227 57 673
Fragments arranged by size:
673 227 57
MslI CAynn'nnrTG
Cuts at: 0 151 184 235 439 592 628 957
Size: 151 33 51 204 153 36 329
Fragments arranged by size:
329 204 153 151 51 36 33
MspA1I CmG'CkG
Cuts at: 0 399 957
Size: 399 558
PasI CC'CwG_GG
Cuts at: 0 456 957
Size: 456 501
PflMI CCAn_nnn'nTGG
Cuts at: 0 149 957
Size: 149 808
PfoI T'CCnGG_A
Cuts at: 0 215 957
Size: 215 742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 684 716 957
Size: 34 32 618 32 241
Fragments arranged by size:
618 241 34 32 32
PspOMI G'GGCC_C
Cuts at: 0 272 305 452 957
Size: 272 33 147 505
Fragments arranged by size:
505 272 147 33
PstI C_TGCA'G
Cuts at: 0 485 767 781 957
Size: 485 282 14 176
Fragments arranged by size:
485 282 176 14
SfcI C'TryA_G
Cuts at: 0 481 763 777 957
Size: 481 282 14 180
Fragments arranged by size:
481 282 180 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 436 791 957
Size: 141 295 355 166
Fragments arranged by size:
355 295 166 141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 957
Size: 150 807
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI BanII BbsI Bme1580I Bpu10I BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BssSI
BstXI Bsu36I BtgI BtgZI CspCI EaeI EarI EcoO109I
EcoRI MmeI MslI MspA1I PasI PflMI PfoI PpiI
PspOMI PstI SfcI TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstYI BstZ17I BtsI ClaI DraI DraIII DrdI EagI
EciI EcoICRI Eco57MI EcoNI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI