Restriction map for
pF1KE0863
(Linear) (Six-base) MAPSORT of: KIEE0863.seq Check: 2423 from: 1 to: 939
KIEE0863.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 730 939
Size: 730 209
AccI GT'mk_AC
Cuts at: 0 101 939
Size: 101 838
Acc65I G'GTAC_C
Cuts at: 0 439 939
Size: 439 500
ApaI G_GGCC'C
Cuts at: 0 76 903 939
Size: 76 827 36
Fragments arranged by size:
827 76 36
ApoI r'AATT_y
Cuts at: 0 31 939
Size: 31 908
BanI G'GyrC_C
Cuts at: 0 439 670 939
Size: 439 231 269
Fragments arranged by size:
439 269 231
BanII G_rGCy'C
Cuts at: 0 76 212 259 415 903 939
Size: 76 136 47 156 488 36
Fragments arranged by size:
488 156 136 76 47 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 303 609 705 939
Size: 303 306 96 234
Fragments arranged by size:
306 303 234 96
BclI T'GATC_A
Cuts at: 0 131 939
Size: 131 808
BglI GCCn_nnn'nGGC
Cuts at: 0 427 939
Size: 427 512
BglII A'GATC_T
Cuts at: 0 590 939
Size: 590 349
Bme1580I G_kGCm'C
Cuts at: 0 76 236 903 939
Size: 76 160 667 36
Fragments arranged by size:
667 160 76 36
BmgBI CAC'GTC
Cuts at: 0 891 939
Size: 891 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 429 939
Size: 43 386 510
Fragments arranged by size:
510 386 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 750 939
Size: 750 189
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 717 789 909 939
Size: 130 587 72 120 30
Fragments arranged by size:
587 130 120 72 30
BseYI C'CCAG_C
Cuts at: 0 4 117 571 939
Size: 4 113 454 368
Fragments arranged by size:
454 368 113 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 314 939
Size: 148 166 625
Fragments arranged by size:
625 166 148
BsiEI CG_ry'CG
Cuts at: 0 787 939
Size: 787 152
BsiHKAI G_wGCw'C
Cuts at: 0 212 939
Size: 212 727
Bsp1286I G_dGCh'C
Cuts at: 0 76 212 236 259 415 903 939
Size: 76 136 24 23 156 488 36
Fragments arranged by size:
488 156 136 76 36 24 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 730 939
Size: 730 209
BsrDI GCAATG_nn'
Cuts at: 0 689 939
Size: 689 250
BssSI C'ACGA_G
Cuts at: 0 739 939
Size: 739 200
BstEII G'GTnAC_C
Cuts at: 0 108 939
Size: 108 831
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 939
Size: 243 696
BstYI r'GATC_y
Cuts at: 0 590 939
Size: 590 349
BtgI C'CryG_G
Cuts at: 0 353 415 431 939
Size: 353 62 16 508
Fragments arranged by size:
508 353 62 16
BtsI GCAGTG_nn'
Cuts at: 0 22 939
Size: 22 917
DraIII CAC_nnn'GTG
Cuts at: 0 915 939
Size: 915 24
EaeI y'GGCC_r
Cuts at: 0 245 350 428 467 784 939
Size: 245 105 78 39 317 155
Fragments arranged by size:
317 245 155 105 78 39
EagI C'GGCC_G
Cuts at: 0 784 939
Size: 784 155
EarI CTCTTCn'nnn_
Cuts at: 0 217 757 835 913 939
Size: 217 540 78 78 26
Fragments arranged by size:
540 217 78 78 26
EcoICRI GAG'CTC
Cuts at: 0 210 939
Size: 210 729
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 939
Size: 145 794
EcoO109I rG'GnC_Cy
Cuts at: 0 63 72 73 239 899 900 939
Size: 63 9 1 166 660 1 39
Fragments arranged by size:
660 166 63 39 9 1 1
EcoRI G'AATT_C
Cuts at: 0 31 939
Size: 31 908
HaeII r_GCGC'y
Cuts at: 0 930 939
Size: 930 9
KpnI G_GTAC'C
Cuts at: 0 443 939
Size: 443 496
MscI TGG'CCA
Cuts at: 0 247 430 939
Size: 247 183 509
Fragments arranged by size:
509 247 183
MslI CAynn'nnrTG
Cuts at: 0 352 430 850 939
Size: 352 78 420 89
Fragments arranged by size:
420 352 89 78
NcoI C'CATG_G
Cuts at: 0 353 431 939
Size: 353 78 508
Fragments arranged by size:
508 353 78
PpuMI rG'GwC_Cy
Cuts at: 0 239 939
Size: 239 700
PspOMI G'GGCC_C
Cuts at: 0 72 899 939
Size: 72 827 40
Fragments arranged by size:
827 72 40
PstI C_TGCA'G
Cuts at: 0 71 939
Size: 71 868
SacI G_AGCT'C
Cuts at: 0 212 939
Size: 212 727
SapI GCTCTTCn'nnn_
Cuts at: 0 217 939
Size: 217 722
SbfI CC_TGCA'GG
Cuts at: 0 71 939
Size: 71 868
ScaI AGT'ACT
Cuts at: 0 554 939
Size: 554 385
SexAI A'CCwGG_T
Cuts at: 0 104 939
Size: 104 835
SfcI C'TryA_G
Cuts at: 0 67 939
Size: 67 872
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 427 939
Size: 427 512
SmlI C'TyrA_G
Cuts at: 0 58 408 939
Size: 58 350 531
Fragments arranged by size:
531 350 58
StyI C'CwwG_G
Cuts at: 0 353 431 939
Size: 353 78 508
Fragments arranged by size:
508 353 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 75 380 490 658 709 864 939
Size: 75 305 110 168 51 155 75
Fragments arranged by size:
305 168 155 110 75 75 51
TatI w'GTAC_w
Cuts at: 0 176 552 939
Size: 176 376 387
Fragments arranged by size:
387 376 176
Tth111I GACn'n_nGTC
Cuts at: 0 817 939
Size: 817 122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 939
Size: 681 258
Enzymes that do cut:
AarI AccI Acc65I ApaI ApoI BanI BanII BbsI
BclI BglI BglII Bme1580I BmgBI BpuEI BsaI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BssSI
BstEII BstXI BstYI BtgI BtsI DraIII EaeI EagI
EarI EcoICRI EcoNI EcoO109I EcoRI HaeII KpnI MscI
MslI NcoI PpuMI PspOMI PstI SacI SapI SbfI
ScaI SexAI SfcI SfiI SmlI StyI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BfrBI BlpI BmrI BmtI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EciI Eco57MI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI MfeI MluI MmeI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XbaI XhoI XmaI
XmnI ZraI