Restriction map for
pF1KE0862
(Linear) (Six-base) MAPSORT of: KIEE0862.seq Check: 4645 from: 1 to: 939
KIEE0862.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 939
Size: 564 375
AflII C'TTAA_G
Cuts at: 0 777 939
Size: 777 162
AhdI GACnn_n'nnGTC
Cuts at: 0 255 939
Size: 255 684
AleI CACnn'nnGTG
Cuts at: 0 145 939
Size: 145 794
AlwNI CAG_nnn'CTG
Cuts at: 0 75 939
Size: 75 864
ApoI r'AATT_y
Cuts at: 0 886 939
Size: 886 53
BanI G'GyrC_C
Cuts at: 0 118 939
Size: 118 821
BanII G_rGCy'C
Cuts at: 0 162 939
Size: 162 777
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 939
Size: 274 665
Bme1580I G_kGCm'C
Cuts at: 0 121 939
Size: 121 818
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 315 498 939
Size: 88 227 183 441
Fragments arranged by size:
441 227 183 88
BsiHKAI G_wGCw'C
Cuts at: 0 404 939
Size: 404 535
Bsp1286I G_dGCh'C
Cuts at: 0 121 162 404 939
Size: 121 41 242 535
Fragments arranged by size:
535 242 121 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 939
Size: 260 679
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 939
Size: 75 864
BstEII G'GTnAC_C
Cuts at: 0 471 939
Size: 471 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 243 939
Size: 150 93 696
Fragments arranged by size:
696 150 93
BtgI C'CryG_G
Cuts at: 0 350 939
Size: 350 589
BtsI GCAGTG_nn'
Cuts at: 0 385 939
Size: 385 554
EaeI y'GGCC_r
Cuts at: 0 59 371 915 939
Size: 59 312 544 24
Fragments arranged by size:
544 312 59 24
EarI CTCTTCn'nnn_
Cuts at: 0 319 939
Size: 319 620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 939
Size: 564 375
EcoRI G'AATT_C
Cuts at: 0 886 939
Size: 886 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 241 273 855 887 939
Size: 20 32 189 32 582 32 52
Fragments arranged by size:
582 189 52 32 32 32 20
HincII GTy'rAC
Cuts at: 0 249 939
Size: 249 690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 675 939
Size: 536 139 264
Fragments arranged by size:
536 264 139
MscI TGG'CCA
Cuts at: 0 61 373 939
Size: 61 312 566
Fragments arranged by size:
566 312 61
MslI CAynn'nnrTG
Cuts at: 0 145 298 598 939
Size: 145 153 300 341
Fragments arranged by size:
341 300 153 145
MspA1I CmG'CkG
Cuts at: 0 75 588 939
Size: 75 513 351
Fragments arranged by size:
513 351 75
NcoI C'CATG_G
Cuts at: 0 350 939
Size: 350 589
PstI C_TGCA'G
Cuts at: 0 71 939
Size: 71 868
PvuII CAG'CTG
Cuts at: 0 75 588 939
Size: 75 513 351
Fragments arranged by size:
513 351 75
SfcI C'TryA_G
Cuts at: 0 67 753 939
Size: 67 686 186
Fragments arranged by size:
686 186 67
SmlI C'TyrA_G
Cuts at: 0 777 939
Size: 777 162
StyI C'CwwG_G
Cuts at: 0 350 617 939
Size: 350 267 322
Fragments arranged by size:
350 322 267
TatI w'GTAC_w
Cuts at: 0 176 939
Size: 176 763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 433 939
Size: 401 32 506
Fragments arranged by size:
506 401 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 279 939
Size: 147 132 660
Fragments arranged by size:
660 147 132
Enzymes that do cut:
AcuI AflII AhdI AleI AlwNI ApoI BanI BanII
BbsI Bme1580I BseRI BsiHKAI Bsp1286I BspMI BstAPI BstEII
BstXI BtgI BtsI EaeI EarI Eco57MI EcoRI Hin4I
HincII MmeI MscI MslI MspA1I NcoI PstI PvuII
SfcI SmlI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XhoI XmaI XmnI ZraI