Restriction map for
pF1KE0861
(Linear) (Six-base) MAPSORT of: KIEE0861.seq Check: 1999 from: 1 to: 828
KIEE0861.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AclI AA'CG_TT
Cuts at: 0 301 828
Size: 301 527
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 794 826 828
Size: 794 32 2
Fragments arranged by size:
794 32 2
AseI AT'TA_AT
Cuts at: 0 566 828
Size: 566 262
BbsI GAAGACnn'nnnn_
Cuts at: 0 630 709 828
Size: 630 79 119
Fragments arranged by size:
630 119 79
BbvCI CC'TCA_GC
Cuts at: 0 684 689 828
Size: 684 5 139
Fragments arranged by size:
684 139 5
BmtI G_CTAG'C
Cuts at: 0 203 828
Size: 203 625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 231 828
Size: 36 195 597
Fragments arranged by size:
597 195 36
Bpu10I CC'TnA_GC
Cuts at: 0 187 684 689 828
Size: 187 497 5 139
Fragments arranged by size:
497 187 139 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 658 828
Size: 408 250 170
Fragments arranged by size:
408 250 170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 794 824 828
Size: 794 30 4
Fragments arranged by size:
794 30 4
BseYI C'CCAG_C
Cuts at: 0 266 828
Size: 266 562
BspHI T'CATG_A
Cuts at: 0 134 548 828
Size: 134 414 280
Fragments arranged by size:
414 280 134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 274 828
Size: 274 554
BsrDI GCAATG_nn'
Cuts at: 0 129 764 828
Size: 129 635 64
Fragments arranged by size:
635 129 64
BsrGI T'GTAC_A
Cuts at: 0 101 215 828
Size: 101 114 613
Fragments arranged by size:
613 114 101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 633 828
Size: 633 195
BtgI C'CryG_G
Cuts at: 0 461 626 828
Size: 461 165 202
Fragments arranged by size:
461 202 165
DraIII CAC_nnn'GTG
Cuts at: 0 513 828
Size: 513 315
EaeI y'GGCC_r
Cuts at: 0 320 371 458 828
Size: 320 51 87 370
Fragments arranged by size:
370 320 87 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 231 828
Size: 36 195 597
Fragments arranged by size:
597 195 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 19 828
Size: 19 809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 723 828
Size: 723 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 828
Size: 383 32 413
Fragments arranged by size:
413 383 32
MfeI C'AATT_G
Cuts at: 0 330 828
Size: 330 498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 828
Size: 676 152
MscI TGG'CCA
Cuts at: 0 322 373 460 828
Size: 322 51 87 368
Fragments arranged by size:
368 322 87 51
MslI CAynn'nnrTG
Cuts at: 0 133 172 349 828
Size: 133 39 177 479
Fragments arranged by size:
479 177 133 39
MspA1I CmG'CkG
Cuts at: 0 429 469 688 828
Size: 429 40 219 140
Fragments arranged by size:
429 219 140 40
NcoI C'CATG_G
Cuts at: 0 461 626 828
Size: 461 165 202
Fragments arranged by size:
461 202 165
NheI G'CTAG_C
Cuts at: 0 199 828
Size: 199 629
NspI r_CATG'y
Cuts at: 0 195 828
Size: 195 633
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 828
Size: 369 459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 794 826 828
Size: 794 32 2
Fragments arranged by size:
794 32 2
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 208 240 828
Size: 208 32 588
Fragments arranged by size:
588 208 32
PvuII CAG'CTG
Cuts at: 0 429 469 688 828
Size: 429 40 219 140
Fragments arranged by size:
429 219 140 40
SfcI C'TryA_G
Cuts at: 0 797 828
Size: 797 31
SmlI C'TyrA_G
Cuts at: 0 387 673 828
Size: 387 286 155
Fragments arranged by size:
387 286 155
SphI G_CATG'C
Cuts at: 0 195 828
Size: 195 633
StyI C'CwwG_G
Cuts at: 0 317 461 626 828
Size: 317 144 165 202
Fragments arranged by size:
317 202 165 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 310 828
Size: 310 518
TatI w'GTAC_w
Cuts at: 0 101 176 215 828
Size: 101 75 39 613
Fragments arranged by size:
613 101 75 39
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 433 828
Size: 433 395
Enzymes that do cut:
AclI AloI AseI BbsI BbvCI BmtI BpmI Bpu10I
BpuEI BsaXI BseYI BspHI BspMI BsrDI BsrGI BstXI
BtgI DraIII EaeI Eco57MI EcoNI FalI Hin4I MfeI
MmeI MscI MslI MspA1I NcoI NheI NspI PflMI
PpiI PsrI PvuII SfcI SmlI SphI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EarI EciI EcoICRI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI