Restriction map for
pF1KE0860
(Linear) (Six-base) MAPSORT of: KIEE0860.seq Check: 9953 from: 1 to: 927
KIEE0860.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 0 101 518 927
Size: 101 417 409
Fragments arranged by size:
417 409 101
AleI CACnn'nnGTG
Cuts at: 0 22 927
Size: 22 905
ApoI r'AATT_y
Cuts at: 0 31 927
Size: 31 896
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 927
Size: 709 218
BbvCI CC'TCA_GC
Cuts at: 0 684 689 927
Size: 684 5 238
Fragments arranged by size:
684 238 5
Bme1580I G_kGCm'C
Cuts at: 0 426 626 927
Size: 426 200 301
Fragments arranged by size:
426 301 200
BmrI ACTGGGnnnn_n'
Cuts at: 0 538 927
Size: 538 389
Bpu10I CC'TnA_GC
Cuts at: 0 187 684 689 927
Size: 187 497 5 238
Fragments arranged by size:
497 238 187 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 927
Size: 760 167
BsaBI GATnn'nnATC
Cuts at: 0 640 927
Size: 640 287
BseYI C'CCAG_C
Cuts at: 0 425 927
Size: 425 502
BsmI GAATG_Cn'
Cuts at: 0 245 927
Size: 245 682
BsmBI CGTCTCn'nnnn_
Cuts at: 0 202 927
Size: 202 725
Bsp1286I G_dGCh'C
Cuts at: 0 426 626 927
Size: 426 200 301
Fragments arranged by size:
426 301 200
BspHI T'CATG_A
Cuts at: 0 404 548 927
Size: 404 144 379
Fragments arranged by size:
404 379 144
BsrDI GCAATG_nn'
Cuts at: 0 356 764 927
Size: 356 408 163
Fragments arranged by size:
408 356 163
BsrGI T'GTAC_A
Cuts at: 0 215 927
Size: 215 712
BstAPI GCAn_nnn'nTGC
Cuts at: 0 289 927
Size: 289 638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 511 546 927
Size: 511 35 381
Fragments arranged by size:
511 381 35
EaeI y'GGCC_r
Cuts at: 0 320 371 927
Size: 320 51 556
Fragments arranged by size:
556 320 51
EarI CTCTTCn'nnn_
Cuts at: 0 669 927
Size: 669 258
EcoO109I rG'GnC_Cy
Cuts at: 0 440 927
Size: 440 487
EcoRI G'AATT_C
Cuts at: 0 31 927
Size: 31 896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 723 927
Size: 723 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 927
Size: 383 32 512
Fragments arranged by size:
512 383 32
MscI TGG'CCA
Cuts at: 0 322 373 927
Size: 322 51 554
Fragments arranged by size:
554 322 51
MslI CAynn'nnrTG
Cuts at: 0 22 133 172 927
Size: 22 111 39 755
Fragments arranged by size:
755 111 39 22
MspA1I CmG'CkG
Cuts at: 0 429 688 927
Size: 429 259 239
Fragments arranged by size:
429 259 239
NspI r_CATG'y
Cuts at: 0 195 927
Size: 195 732
PflMI CCAn_nnn'nTGG
Cuts at: 0 505 927
Size: 505 422
PpuMI rG'GwC_Cy
Cuts at: 0 440 927
Size: 440 487
PstI C_TGCA'G
Cuts at: 0 382 927
Size: 382 545
PvuII CAG'CTG
Cuts at: 0 429 688 927
Size: 429 259 239
Fragments arranged by size:
429 259 239
SfcI C'TryA_G
Cuts at: 0 378 927
Size: 378 549
SmlI C'TyrA_G
Cuts at: 0 775 927
Size: 775 152
SphI G_CATG'C
Cuts at: 0 195 927
Size: 195 732
SspI AAT'ATT
Cuts at: 0 921 927
Size: 921 6
StuI AGG'CCT
Cuts at: 0 632 927
Size: 632 295
StyI C'CwwG_G
Cuts at: 0 278 927
Size: 278 649
TatI w'GTAC_w
Cuts at: 0 215 927
Size: 215 712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 433 927
Size: 433 494
XmnI GAAnn'nnTTC
Cuts at: 0 921 927
Size: 921 6
Enzymes that do cut:
AccI AleI ApoI BbsI BbvCI Bme1580I BmrI Bpu10I
BpuEI BsaBI BseYI BsmI BsmBI Bsp1286I BspHI BsrDI
BsrGI BstAPI CspCI EaeI EarI EcoO109I EcoRI FalI
Hin4I MscI MslI MspA1I NspI PflMI PpuMI PstI
PvuII SfcI SmlI SphI SspI StuI StyI TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpmI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI ZraI