Restriction map for
pF1KE0855
(Linear) (Six-base) MAPSORT of: KIEE0855.seq Check: 1917 from: 1 to: 933
KIEE0855.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AflIII A'CryG_T
Cuts at: 0 203 933
Size: 203 730
AvaI C'yCGr_G
Cuts at: 0 618 933
Size: 618 315
BamHI G'GATC_C
Cuts at: 0 677 933
Size: 677 256
BanI G'GyrC_C
Cuts at: 0 225 933
Size: 225 708
BbsI GAAGACnn'nnnn_
Cuts at: 0 75 933
Size: 75 858
BbvCI CC'TCA_GC
Cuts at: 0 573 933
Size: 573 360
BclI T'GATC_A
Cuts at: 0 137 933
Size: 137 796
BglI GCCn_nnn'nGGC
Cuts at: 0 427 933
Size: 427 506
BlpI GC'TnA_GC
Cuts at: 0 293 933
Size: 293 640
Bme1580I G_kGCm'C
Cuts at: 0 230 423 933
Size: 230 193 510
Fragments arranged by size:
510 230 193
BmrI ACTGGGnnnn_n'
Cuts at: 0 416 933
Size: 416 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 933
Size: 771 162
Bpu10I CC'TnA_GC
Cuts at: 0 385 573 923 933
Size: 385 188 350 10
Fragments arranged by size:
385 350 188 10
BsaAI yAC'GTr
Cuts at: 0 99 933
Size: 99 834
BsaBI GATnn'nnATC
Cuts at: 0 589 933
Size: 589 344
BsaHI Gr'CG_yC
Cuts at: 0 65 794 933
Size: 65 729 139
Fragments arranged by size:
729 139 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 819 849 933
Size: 819 30 84
Fragments arranged by size:
819 84 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 827 933
Size: 112 715 106
Fragments arranged by size:
715 112 106
BseYI C'CCAG_C
Cuts at: 0 111 933
Size: 111 822
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 584 933
Size: 442 142 349
Fragments arranged by size:
442 349 142
BsiHKAI G_wGCw'C
Cuts at: 0 104 325 933
Size: 104 221 608
Fragments arranged by size:
608 221 104
Bsp1286I G_dGCh'C
Cuts at: 0 104 230 325 423 933
Size: 104 126 95 98 510
Fragments arranged by size:
510 126 104 98 95
BsrBI CCG'CTC
Cuts at: 0 384 933
Size: 384 549
BsrFI r'CCGG_y
Cuts at: 0 433 933
Size: 433 500
BsrGI T'GTAC_A
Cuts at: 0 857 933
Size: 857 76
BssSI C'ACGA_G
Cuts at: 0 414 933
Size: 414 519
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 933
Size: 585 348
BstYI r'GATC_y
Cuts at: 0 515 677 933
Size: 515 162 256
Fragments arranged by size:
515 256 162
Bsu36I CC'TnA_GG
Cuts at: 0 775 933
Size: 775 158
DraIII CAC_nnn'GTG
Cuts at: 0 329 933
Size: 329 604
DrdI GACnn_nn'nnGTC
Cuts at: 0 249 933
Size: 249 684
EaeI y'GGCC_r
Cuts at: 0 368 428 812 933
Size: 368 60 384 121
Fragments arranged by size:
384 368 121 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 933
Size: 771 162
HaeII r_GCGC'y
Cuts at: 0 414 933
Size: 414 519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 933
Size: 29 904
MscI TGG'CCA
Cuts at: 0 370 430 933
Size: 370 60 503
Fragments arranged by size:
503 370 60
MslI CAynn'nnrTG
Cuts at: 0 166 202 346 397 595 933
Size: 166 36 144 51 198 338
Fragments arranged by size:
338 198 166 144 51 36
MspA1I CmG'CkG
Cuts at: 0 284 933
Size: 284 649
NspI r_CATG'y
Cuts at: 0 207 933
Size: 207 726
PasI CC'CwG_GG
Cuts at: 0 55 933
Size: 55 878
PciI A'CATG_T
Cuts at: 0 203 933
Size: 203 730
PflMI CCAn_nnn'nTGG
Cuts at: 0 76 315 933
Size: 76 239 618
Fragments arranged by size:
618 239 76
PvuII CAG'CTG
Cuts at: 0 284 933
Size: 284 649
SspI AAT'ATT
Cuts at: 0 603 933
Size: 603 330
StyI C'CwwG_G
Cuts at: 0 245 933
Size: 245 688
TatI w'GTAC_w
Cuts at: 0 170 857 933
Size: 170 687 76
Fragments arranged by size:
687 170 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 59 530 562 819 851 933
Size: 27 32 471 32 257 32 82
Fragments arranged by size:
471 257 82 32 32 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 933
Size: 552 381
Enzymes that do cut:
AflIII AvaI BamHI BanI BbsI BbvCI BclI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI
BsrGI BssSI BstXI BstYI Bsu36I DraIII DrdI EaeI
Eco57MI HaeII MmeI MscI MslI MspA1I NspI PasI
PciI PflMI PvuII SspI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BanII BbeI
BcgI BciVI BfrBI BglII BmgBI BmtI BplI BpuEI
BsaI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BssHII BstAPI BstBI BstEII BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI