(Linear) (Six-base) MAPSORT of: KIEE0852.seq  Check: 2034  from: 1  to: 894



KIEE0852.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     721     894 
   Size:        721     173
 
AfeI AGC'GCT

Cuts at:      0     720     894 
   Size:        720     174
 
AleI CACnn'nnGTG

Cuts at:      0      16     894 
   Size:         16     878
 
ApoI r'AATT_y

Cuts at:      0      25     894 
   Size:         25     869
 
AvrII C'CTAG_G

Cuts at:      0     312     894 
   Size:        312     582
 
BlpI GC'TnA_GC

Cuts at:      0     681     894 
   Size:        681     213
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     779     894 
   Size:        779     115
 
Bpu10I CC'TnA_GC

Cuts at:      0     573     894 
   Size:        573     321
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      78     135     894 
   Size:         78      57     759

  Fragments arranged by size: 

                 759      78      57
 
BseYI C'CCAG_C

Cuts at:      0     290     894 
   Size:        290     604
 
BspHI T'CATG_A

Cuts at:      0     398     894 
   Size:        398     496
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     721     894 
   Size:        721     173
 
BsrBI CCG'CTC

Cuts at:      0     680     894 
   Size:        680     214
 
BstEII G'GTnAC_C

Cuts at:      0     828     894 
   Size:        828      66
 
Bsu36I CC'TnA_GG

Cuts at:      0     862     894 
   Size:        862      32
 
BtgI C'CryG_G

Cuts at:      0     428     894 
   Size:        428     466
 
BtsI GCAGTG_nn'

Cuts at:      0     548     728     894 
   Size:        548     180     166

  Fragments arranged by size: 

                 548     180     166
 
DraIII CAC_nnn'GTG

Cuts at:      0     411     894 
   Size:        411     483
 
EaeI y'GGCC_r

Cuts at:      0     425     894 
   Size:        425     469
 
EarI CTCTTCn'nnn_

Cuts at:      0     219     894 
   Size:        219     675
 
EcoO109I rG'GnC_Cy

Cuts at:      0      57     434     894 
   Size:         57     377     460

  Fragments arranged by size: 

                 460     377      57
 
EcoRI G'AATT_C

Cuts at:      0      25     894 
   Size:         25     869
 
HaeII r_GCGC'y

Cuts at:      0     722     894 
   Size:        722     172
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     377     409     456     488     865     894 
   Size:        377      32      47      32     377      29

  Fragments arranged by size: 

                 377     377      47      32      32      29
 
MscI TGG'CCA

Cuts at:      0     427     894 
   Size:        427     467
 
MslI CAynn'nnrTG

Cuts at:      0      16     166     343     894 
   Size:         16     150     177     551

  Fragments arranged by size: 

                 551     177     150      16
 
MspA1I CmG'CkG

Cuts at:      0     699     894 
   Size:        699     195
 
NdeI CA'TA_TG

Cuts at:      0     370     894 
   Size:        370     524
 
PpuMI rG'GwC_Cy

Cuts at:      0     434     894 
   Size:        434     460
 
PstI C_TGCA'G

Cuts at:      0     543     718     894 
   Size:        543     175     176

  Fragments arranged by size: 

                 543     176     175
 
SfcI C'TryA_G

Cuts at:      0     539     714     855     894 
   Size:        539     175     141      39

  Fragments arranged by size: 

                 539     175     141      39
 
StyI C'CwwG_G

Cuts at:      0     312     894 
   Size:        312     582
 
TatI w'GTAC_w

Cuts at:      0     319     894 
   Size:        319     575
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     201     894 
   Size:        201     693
 

 Enzymes that do cut:

     AarI     AfeI     AleI     ApoI    AvrII     BlpI     BmrI   Bpu10I
    BseRI    BseYI    BspHI    BspMI    BsrBI   BstEII   Bsu36I     BtgI
     BtsI   DraIII     EaeI     EarI EcoO109I    EcoRI    HaeII    Hin4I
     MscI     MslI   MspA1I     NdeI    PpuMI     PstI     SfcI     StyI
     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI